BMS C2 MicroscopeOperation InstructionThis manual is written for BMS C2 BIOLOGICAL. For safety and for exerting the bestperformance, making you familiar with the instrument entirely, it is strongly recommended thatyou read this manual carefully before using the microscope. For the further reference, please placethis manual in a position where nearby the worktable and fetched easily.
Follow the Instruction Below1. PurposeThis microscope is only applied for microscopy observation, not for others.2. Never disassembleDisassembly may cause electrical shocks and/or severe damage to the instrument. Neverdisassemble any part than that mentioned on this manual. If you notice any malfunction,contact your nearest representative. Fungicide is used in the eyepiece tube, eyepiecesand objectives. If you should disassemble them and touch the parts inside, you may get arash with the fungicide3. Heat at the Light SourceThe light bulb and the lamp housing will be very hot after long hour working. Never touchthem by hands until they cool down, otherwise your hands will be hurt.4. Power supplyPay attention to the specifications of power supply, which marked on the microscope base.The power supply must comply with the specifications; otherwise the microscope will bedamaged.5. Changing the Light bulbBefore replacing the light bulb with new one, first turn off the light switch on themicroscope base, then take off the power plug and wait until the bulb cools down. Onlyhalogen bulb 6V/20W is workable on this microscope, never use other bulbs.6. Storage1) Before moving the microscope, turn off the light switch and take off the power plug.2) Usually, following environment is required: lndoor temperature: 0 degree ~40 degreeMax relative humidity: 85%3) After use, cover the microscope with the dust-cover provided, and store it in a dry andclean place free from moisture to prevent rust.7. Handle GentlyMicroscope is a precision instrument. Handle it gently. Strong shocks and forcibleoperations will cause severe damage to the instrument.
AssemblyNoteTake care on the following points when installing the microscope.• Be careful not to get your fingers caught under the microscope.• Place the microscope in the following environment.Room temperature: 0℃ to 40℃Relative humidity: max.85%High temperature and humidity will cause molds, dew formation and damage.• Avoid placing the microscope under the direct sunlight or the room light. Brightcircumstances deteriorate the specimen image.• Avoid placing the microscope in dusty surroundings. When the microscope is not inuse, cover it up with a vinyl cover.
• Place the microscope flat on the place free of vibration. LampNote• Lamp and vents are very hot during and right after the illumination. Be careful notto get yourself burnt.• Use the Halogen lamp bulb only. If the bulb burn out, contact your representativefor the replacement.• Do not touch the glass part of the bulb by bare hands. Wear gloves or keep theprotecting cover on the bulb during installation. Wipe off any fingerprints or stainsusing clean cloth moistened with pure alcohol since those burned into the bulbsurface will decrease brightness and cause lamp breakage.• Pull up the lamp cover clamp knob and remove the cover. Turn out theheat-resisting plate and securely insert the lamp's contact pins into the socketholes. Return the plate and the cover to their original place Eyepiece tubeMount the eyepiece tube onto the arm, and tighten the clamp screw. EyepiecesInsert the eyepieces into the eyepiece sleeves, and tighten the clamp screw. ObjectivesLower the stage to its full limit, then screw the objectives into the revolving nosepiece. Daylight FilterPress the filter into the bottom of the auxiliary lens, and then insert the auxiliary lens intothe bottom of the condenser.The phase-contrast observation GIF filter can be placed directly on the field lens unitwhenever necessary. CondenserMount the condenser on the condenser holder with the aperture control lever facingforward. Secure with the clamp screw Power CordTurn OFF the power switch before connecting the power cord.(Turn the Brightness control dial toward the rear of the microscope till the limit.)Insert gently the cable-plug into the socket built-in the microscope base, then insert thepower plug into the socket of power supply.
MICROSCOPY1. Lamp illuminationTurn the brightness control dial forward to achieve the desiredbrightness.2. Specimen MountingMounting with the cover glass facing upwards.5. Eyepiece Diopter AdjustmentFirstly, observe at right tube with right eye, adjust coarse-finefocusing knob to image clearly.Secondly, observe at left tube with left eye, adjust the dioptercontrol 1 to image clearly.6. Objective SelectionRotate the revolving nosepiece to the desired objectivemagnification, assuring it click-stops into position.3. Focusing with 10X ObjectivesChoose the objective 4X or 10X. Turn the coarse focusingknob to get the objective focused on the slide and then turnthe fine focus adjustment knob to get the image sharp andclear.7. Aperture Diaphragm Adjustment(This diaphragm is designed for numerical apertureadjustment, not brightness.)4.Interpupillary Distance AdjustmentAdjust the interpupillary distance of binocular until theright-left field of view can be composed one.General, a good image of sufficient contrast can be achievedwith the aperture diaphragm stopped down to approximately70-80% of the objective’s numerical aperture. To observe the
diaphragm image, remove the eyepiece and look down theopen tube at the exit pupil of the objective.9. Oil-immersion Observation8. Field Diaphragm Adjustment(For the field lens unit with Field Diaphragm)When using the 100X objectives, it is an oil-immersion typerequiring the application of immersion oil between its lens tipand the coverglass.If air bubbles enter the oil layer, a poor image will result. Toremove the air bubbles, rotate the nosepiece several times, orapply an additional drop of oil.After completing oil-immersion observation, be sure to cleanthe front lens of the objective and any other parts coated withoil.Stop down the field diaphragm until the illuminated areaclosely circumscribes the eyepiece viewfield.The field diaphragm is used to control the specimen’silluminated area relative to the microscope viewfield. If it isopened to a larger aperture than necessary, extraneous lightwill enter the viewfield, reducing image quality and contrast.
Troubleshooting TablesIf difficulties should be encountered in the course of operation, and no major instrument malfunctions caninitially be detected, please recheck the symptoms, referring to the tables provided below, before contactingyour service representative.1. OpticalSymptoms Causes CountermeasuresRevolve to click-stop positionRevolving nosepiece not in click-stop(swing the objective correctly intoposition(objective not centered optical path)Darkness at thethe optical path)periphery, or uneven Field diaphragm not centeredCenteringviewfield brightness. Field diaphragm closed too farOpen properlyDirt or dust on the lens (condenser,objective, eyepiece, slide)CleaningDirt or dust in theviewfieldPoor image quality(low resolution orcontrast)Image dark on one sideDirt or dust on the lens (condenser,objective, eyepiece, slide)Dirt or dust on the slide.Condenser position too lowNo coverglass attached to the slideCleaningCleaningCorrect positioningAttach coverglassCoverglass too thick or thin Use coverglass of specifiedthickness(0.17mm)Slide upside-down.Turn over the slideImmersion oil on dry system objective(especially 40X)CleaningDirt or dust on the lens (condenser,objective, eyepiece, slide)No immersion oil used on immersion systemobjectiveAir bubbles in immersion oilCondenser aperture and field diaphragmopened too farDirt or dust on the entrance lens.Condenser aperture closed too farCondenser position too lowRevolving nosepiece not in click-stoppositionFloating specimenImage shifts during Specimen rises from stage surfacefocus Revolving nosepiece not in click-stoppositionCleaningUse immersion oilRemove bubblesClose properlyCleaningOpen properlyRaise to the position where thediaphragm image is in focusRevolve to click-stop positionFasten securelyPlace it stableRevolve to click-stop positionImage tinged yellow Daylight filter not used Use daylight filter
Symptoms Causes CountermeasuresInsufficient illumination Condenser aperture too smallReadjust aperturebrightnessCondenser position too lowCorrect positioningDirt or dust on the lens (condenser,objective, eyepiece, slide)Cleaning2. MechanicalSymptoms Causes CountermeasuresSpecimen image Mechanical stage not securely fastened Tighten all fastenersmovement unsmoothImage not focusablewith high powerobjectivesHigh power objectivecontacts slide whenchanged-over from lowpowerSpecimen imagemovement unsmoothBinocular image notintegratedExcessive eye fatigueSlide upside-downCoverglass too thickSlide upside-downCoverglass too thickMechanical stage not securely fastenedInterpupillary distance not correctly adjustedIncorrect diopter adjustmentInadequate brightness or illuminationTurn slide overUse coverglass of specifiedthickness(0.17mm)Turn slide overUse coverglass of specifiedthickness(0.17mm)Tighten all fastenersAdjustmentCorrect adjustmentAdjust brightness with controldial3. ElectricalSymptoms Causes CountermeasuresNo electrical powerCheck power cord connectionLamp bulb not insertedInsert correctlyLamp does not lightReplacementwhen switched ONIf the same symptom occurs afterLamp failurereplacing the lamp, contact yourdealerUnspecified lamp bulb usedReplace with specified lamp bulbInsufficient illuminationIncrease brightness with controlbrightnessVoltage too lowdialUnstable lampbrightnessLamp bulb about to failReplacementLamp bulb not correctly inserted into socketCheck for positive connection
Care and Maintenance1. Cleaning Lenses• Dust is best removed with a soft brush or gauze.• More persistent dirt, such as fingerprints, grease and oil, may be removed with soft cotton, lens tissue, orgauze lightly moistened with absolute alcohol (ethyl alcohol or methyl alcohol).• To clean immersion oil off the oil-immersion type objective, use lens tissue, soft cotton or gauze lightlymoistened with petroleum benzine only.If petroleum benzene is not available, use methyl alcohol. In this case, you need to wipe three or four timesbecause the detergency of the methyl alcohol is somewhat weak.• Do not use petroleum benzine to clean the entrance lens at the bottom of the eyepiece tube or prismsurfaces inside the eyepiece tube.• Absolute alcohol and petroleum benzine are quite inflammable. Take great care when handling them andwhen setting the power switch on and off. Be very careful with fire2. Cleaning Painted or Plastic Surfaces• Avoid use of any organic solvents (such as alcohol, ether, thinner, etc.) to clean the painted or plasticsurfaces of the instrument. We recommend the use of silicon cloth. More persistent did may be cleaned withmild detergent solution.• Printed plastic surfaces should be cleaned only by soft cloth moistened with water.3. When Not in Use• When the microscope is not in use, cover it up with a vinyl cover, and store it in a dry place not subject tomold.• We especially recommend that the objectives and eyepieces be kept in a container (such as adesiccators )with desiccant in it4. Periodical Inspection• To maintain the performance of the microscope, periodical inspection is recommended. (Contact yournearest representative for details.)