WORKING PARTY REPORTFELASA recommendations for the healthmonitoring of breeding colonies andexperimental units of cats, dogs and pigsReport of the Federation of European Laboratory Animal ScienceAssociations (FELASA)Working Group on Animal HealthFELASAWorking Group on Animal Health: C. Rehbinder (Scand-LAS), Convener;P. Baneux (SFEA); D. Forbes (LASA); H. van Herck (NVP); W. Nicklas (GV-SOLAS);z. Rugaya (Balt-LASA); G.Winkler (SGV)FELASA, BCM Box 2989, LondonWC1N3XX, UKContentsIntroduction1 General considerations2 Inspection of the colony3 Monitoring procedures4 Health monitoring report5 Cat6 Dog7 PigReferencesFELASA-approvedhealth monitoring report forms12233567910Introd uctionThe health of an animal is always at riskfrom a variety of infections. Infections inanimals, whether clinically manifest orsubclinical may, when the animals are usedin biomedical research, produce effects thatchange the outcome of the experimentsundertaken. Depending upon the specificinfection a variety of biological parametersmay be affected such as behaviour, growthrate, relative organ weights, immune response,tumour development etc. Subclinicalinfections can also lead to contamination ofNote: Reprints of this Report are available free of charge(while stocks last) from the Secretary, FELASA, BCM Box2989, London WC1N 3XX, UKbiological materials, tissue cultures, celllines,transplantable tumours and biologicalproducts. All infections, apparent or inapparent,are likely to increase biologicalvariability. In addition, some animal infectionsare transmissible to man.For all these reasons, an animal healthmonitoring programme is important, decreasingthe risk of zoonotic infection andadding to the reliability and reproducibility ofresearch data. These recommendations propose·suchprogrammes for pigs, dogs and cats,specifically bred and used for biomedical'research, with the intention of harmonizingprocedures and achieving similar standards oftesting within the FELASAmember countries.Another goal of these recommendationsis to ensure that health monitoringLaboratory Animals (1998) 32,1-17
FELASAWorkingGroup on Animal Health3Table 1Health monitoring of laboratory cats, dogs and pigs: sample size and frequencySample sizeTesting/animalSampling frequency Age No. of animals Viruses Bacteria ParasitesEvery 3 months Weanlings ~2 +* +t2-7 months* ~4 + + +~8 months* ~4 + + +*If not available. increase the number of samples from the other age group(s)tlf not available at the time of scheduled testing. test for parasites later when available3. Monitoring procedures3.1 Laboratory investigationsAll samples obtained in connection withroutine health monitoring are to be takenfrom live animals. However, additionalsamples may be obtained from dead oreuthanized animals. Samples (bacteriology,serology, parasitology) are preferably monitoredindividually (see Table 1).3.2 The scope of the screening programmeA minimum of 10 animals, randomlyselected, should be sampled at least everythree months or according to the respectivenational disease control programmes andimport/export regulations.Infectious diseases that do not need to bemonitored are those included in an official,national governmental screening programme(but with the results included in the healthmonitoring report), diseases officially declaredabsent in that region and diseases forwhich the animals are vaccinated.Some agents are to be monitored onrequest or• when associated with lesions• when associated with clinical signs ofdisease• when there is evidence of perturbation ofphysiological or experimental parametersand/or breeding performance.4. Health monitoring reportThe main purpose of the health monitoringof experimental units is to supply investigatorswith data on variables that mightinfluence the outcome of an experiment.These data are part of the experimental workand have to be considered during the interpretationof the experimental results by theinvestigator and by the readers of a publication.Results of health monitoring should,therefore, be included in scientific publications.While FELASAcannot accept responsibilityfor tests or their implications,breeders or users of laboratory animals whoare reporting the health monitoring of theiranimals may use the words 'in accordancewith FELASArecommendations' but onlywhere that is in fact the case. The reportshould also include, when related to colonywidemeasures, a note of the occasional orregular use of antibiotics and other microbiologicallyactive substances.4.1 General information on each reportThe title of the report should be FELASA-Approved Health Monitoring Report.This wording can only be used if themethods, frequency, sample size, species-listof organisms monitored and reported are infull accordance with the recommendationspublished by FELASA.The design of thereport could be changed, but only if itincorporates the data requested in therecommendations. At the top of each reportshould be: date of the report, date animalstested, the species and breed, the identificationof the colony or unit, the date when thecolony was established and month and yearwhen it was last rederived or restocked.Description of the strain/stock screened isas follows: name of the species, followed bythe current accepted nomenclature.4.2 Lay-out of the report with respect tomicroorganisms monitored and the colonystatusExcept for general information (see section4.1) the report is divided into five columns,
4FELASAWorkingGroup on Animal Healththe first listing the microorganisms monitored,the second recording the historicalstatus of the colony (section 4.4L the thirdgiving the results of the current screen(section 4.5) the fourth recording the laboratorycarrying out the test and the fifth columnshowing the method used (section 4.3). Allsamples should be monitored individually.Species names of microorganisms should beused in preference to the more general genericnames. The suggested test methods are givenas illustrations of current available techniques.In general the most appropriate andupdated methods should be used.4.3 Listing of microorganisms, methodsand names of monitoring laboratoriesThe organisms detailed in these recommendationsshould be listed alphabetically intheir appropriate sections in the order: ·lstsection: viruses; 2nd section: bacteria, mycoplasma,and fungi; 3rd section: parasites.Current accepted abbreviations for microorganismsmay be used in the report. The fullor abbreviated name of the laboratory carryingout the test must be recorded for eachorganism/agent, but where it is abbreviatedthe full name must be given at the bottom ofthe report.Where both a method and laboratory nameare to be recorded, they should be in theorder: microorganism, laboratory, method(Rehbinder et al. 1996).4.4 Historical status of the colonyAgainst each organism must be recorded:Pos if the organism has ever been detected(i.e. positive).Neg if the organism has never been detectedin previous screens (i.e. negative).NE if the organism has not been includedin the health monitoring programme(i.e. not examined).4.5 Current health monitoring resultsEach organism must be recorded:Pos/testedNegNEif the organism has been detectedin the current screen ofanimals (number of animalspositive out of numbers tested).if the organism has not beendetected in the current screen ofanimals.if the organism has not beenexamined for in the currentscreen of animals.The results of special investigations ofunusual or unexpected occurrences should bereported separately.4.6 Additional informationAny additional information should be givenon a separate sheet accompanying the mainreport and not on the FELASA-ApprovedHealth Monitoring Report itself. If anTable 2 Monitoring of viral infections (cat)List of viral infections to be serologically monitored:VirusFeline calicivirusFeline immunodeficiency virus (FIV)Feline infectious peritonitis virus (coronavirus) (FIP)Feline parvovirusFeline rhinotracheitis virusSuitableNTELISA,WesternELISA, peRELISANTtest methodsblotList of viral infections to be monitored by other methods:AntigenSuitabletest methodsFeline intestinal coronavirusFeline leukaemia virus (FeLV)RotavirusDetection of antigen in faeces by ELISA; EM orlatex-agglutinationDetection of antigen in serum by ELISADetection of antigen in faeces by ELISA; EM orlatex-agglutinationELlSA=enzyme-linked immunosorbent assay; EM=electron microscopy; IFA=immunofluorescence assay; NT=neutralizationtest; PCR=polymerase chain reaction
FELASAWorkingGroup on Animal Health5Table 3 Monitoring of bacterial infections (cat)List of bacterial and fungal infections to be monitored compulsorily:Agent/AntigenSuitabletest methodBartonella spp.Bordetella bronchisepticaCampy/obacter spp.Chlamydia psittaciMicrosporum spp.Pasteu rellaceaeSalmonella spp. .Staphylococcus spp. (when associated with lesions)Streptococci beta-haemolytic serogroup GTrichophyton spp.Yersinia enterocoliticaBacterial infection to be monitored on request:AgentCultureCultureCultureSerologyCultureCultureCultureCultureCultureCultureCultureSuitabletest methodHe/icobacterspp.CultureTable 4 Monitoring of parasites (cat) Table 5 Monitoring of viral infections (dog)Compulsorylist of parasites to be monitored:All arthropodsAll helminthsEperythrozoon fe/isHaemobartonella felisIsospora spp.Sarcocystis spp.Toxoplasma gondiiExamples of parasites to be monitoredGiardia spp.Ollu/anus tricuspis (necropsy)*on request:*Histopathological evaluation of gastric mucosa whenavailable due to death or from euthanasia or other causesinfection is discovered outside of the routinemonitoring schedule, users should be informedimmediately.5. CatViral infections (Table 2)Equivocal or unexpected positive serologicaltest results must be confirmed by an alternativetest method and/or repeated investigation.Bacterial and fungal infectionsCulturing is the method of choice unlessotherwise stated. Bacteriological investigationsmust always include the use of nonselective,as well as selective, media.List of viral infections to be serologically monitoredwhen present in the country:Virus Suitable test methodsCanine adenovirus type 1(HCC)Canine distemper virusCanine parainfluenza virusCanine parvovirus (CPV)List of viral infections to be monitored on request byother methods:Antigen Suitable test methodsIntestinal coronavirus whenassociated with diseaseRotavirus, when associatedwith diseaseCF, NTELISA, NT, IFAELISA, HIELISA, HIDetection of antigen infaeces by ELISA; EM orlatex-agglutinationDetection of antigen infaeces by ELISA; EM orlatex-agglutinationCF=complement fixation test; ELlSA=enzyme linked immunosorbentassay; EM=electron,microscopy; HI=haemagglutinationinhibition test; IFA=immunofluorescenceassay; NT=neutralization testSerological methods exist for the detection ofantibodies to various pathogens.Samplesto be investigatedSamples from the following sites must becultured: tonsillary region (swabl, skin/hair(combed sample), faeces (fresh faecal materialcollected by a suitable method) (Table 3).
6 FELASAWorking Group on Animal HealthTable 6Monitoring of bacterial infections (dog)Compulsory list of bacterial infections to be monitored:Agent/Antigen Suitable methodBordetella bronchisepticaBorrelia spp.Brucella canisLeptospira spp.Salmonella spp.Streptococci beta-haemolytic, serogroup GCultureSerologyCultureSerologyCultureCultureBacterial and fungal infections to be monitored on request or when associatedwith lesions or clinical signs:Agent/Antigen Suitable test methodCampy/obacter spp.Ehrlichia spp.Escherichia coliMicrosporum spp.PasteurellaceaeStaphylococcus spp.Trichophyton spp.Yersinia enterocoliticaCultureSerology,CultureCultureCultureCultureCultureCulturePCRParasitologyRoutine methodology.Faecal flotation.Microscopic examination of wet mounts.Microscopic examination for Otodectescynotis.Blood smears stained with May-Griinwald-Giemsa for the screening of Haemobar- .tonella felis.Serum samples examined for the presence ofantibodies to Toxoplasma gondii.The organisms in Table 4 must be includedin the final report of results, with a declarationof whether they have been detected ornot (numbers of animals positive), or notexamined (Table 4).6. DogViral infections (Table 5)Bacterial and fungal infectionsCulturing is the method of choice unlessotherwise stated. Bacteriologicalinvestigations must always include the useTable 7Monitoring of parasites (dog)Compulsory list of parasites to be monitored:All arthropods: (Demodex sp., dermal scrapings only when associated with lesions,Sarcoptes scabei, serology and/or dermal scrapings)All heminthsCoccidiaeGiardiaspp.Haemobartonella canis: blood smearsExamples of parasites to be monitored on request:Angiostrongy/usvasorumBabesia spp.: serology, blood smearDipetalonema reconditum: blood smearDirofilaria immitis: blood smearFilaroides spp. *leishmania spp.: serologyPneumonyssus caninum: serology or direct examination at necropsy* Histopathological evaluation for FiJaroides spp. in lung tissue when available due to death orfrom euthanasia for other causes
FELASAWorkingGroup on Animal Health7Table 8Monitoring of viral infections (pig)List of viral infections to be serologically monitored, when present in the country (see 1.1):Virus Suitable test methodAfrican swine feverAujeszky disease virus (pseudorabies)Classical swine fever (hog cholera)Encephalomyocarditis virusHaemagglutinating encephalomyelitisPorcine cytomegalovirus (inclusion body rhinitis)Porcine influenza (H1N1), (H3N2)Porcine parvovirusPorcine reproductive and respiratory syndrome (PRRS)SMEDITeschen/Talfan disease virusTransmissible gastroenteritis (TGE)ELISAELISAELISAELISA, PCRHA, NT, ELISANTELISA, HIELISA, HIELISANTIFA,NTELISAList of viral infections to be monitored by other methods:Antigen Suitable test methodPorcine epidemic diarrhoea (when associated withdisease)Porcine rotavirusDetection of antigen in faeces byELISA; EM or latex-agglutinationDetection of antigen in faeces byELISA; EM or latex-agglutinationExamples of viral infections to be monitored on request and when present in the country:Antigen Suitable test methodFoot and mouth disease virus (FMD)Porcine respiratory coronavirusSwine vesicular disease virus (SVDV)Vesicular exanthema virus (VEV)Vesicular stomatitis virus of swine (VSVS)ELISAELISAELISANTNTELlSA=enzyme linked immunosorbent assay; EM=electron microscopy; HA=haemagglutinationtest; HI=haemagglutination inhibition test; IFA=immunofluorescence assay; NT=neutralizationtest; PCR=polymerase chain reactionof non-selective, as well as selective, media.Serological methods exist for the detection ofantibodies to various pathogens e.g. Leptospiraspp., Borrelia spp. and Ehrlichia canis.Other validated methods may be used.Samplesto be investigatedSamples from the following sites must becultured: tonsillary region (swabl, skin/hair(combed samplel, faeces (fresh materialcollected by a suitable methodl (Table 6).ParasitologyFaecal flotation and sedimentation.Microscopic examination of wet mounts.Microscopic examination for Otodectescynotis.Blood smears stained with May-Griinewald-Giemsa for the screening of Haemobartonellacanis (Table 7).Special attention should be given to ectoparasitessuch as fleas, lice, ticks and mites.Inspection should be performed at an appropriatetime after any us.e of an ectoparasiticide.7. PigViral infections (Table 8)Equivocal or unexpected positive serologicaltest results must be confirmed by an alternativetest method and/or repeated investigation.Bacterial, mycoplasmal and fungalinfectionsCulturing is the method of choice unlessotherwise stated. Bacteriologicalinvestigations must always include the use
8FELASAWorkingGroup on Animal HealthTable 9Monitoring of bacterial infections (pig)List of bacterial and mycoplasmal infections to be monitored compulsorily:Agent/Antigen Suitable test methodActinobacillus pleuropneumoniaeBordetella bronchisepticaErysipelothrix rhusiopathiaeEubacterium (Corynebacterium) suisHaemophi/us parasuisLeptospira spp.Mycoplasma hyopneumoniaePasteurella multocida (toxin producing)Salmonella spp.Staphylococcus hyicusStreptococci beta-haemolyticStreptococcus suisYersinia enterocoliticaSerologyCultureCulture,CultureCulture,SerologyCulture,serologyserologyserologyCulture, serology, demonstration of toxinby ELISACultureCulture when associated with skin lesionsCulture, designation of Lancefield groupif possibleCultureCultureExamples of bacterial and fungal infections to be monitored on request:Agent/Antigen Suitable test methodActinomyces pyogenesBrucella suisClostridium perfringensEscherichia coli when associated with enteric diseaseMicrosporum spp.Serpulina hyodysenteriaeTrichophyton spp.CultureCultureCultureCulture, designation of serotype ifpossibleCultureCulture and serologyCultureof non-selective, as well as selective, media.Serological methods exist for the detection ofantibodies to various pathogens e.g. Actinobacilluspleuropneumoniae, Haemophilusparasuis, Leptospira spp., Mycoplasmahyopneumonia and others.Samplesto be investigatedSamples from the following sites must becultured: nose (swabl, faeces (fresh faecalmaterial collected by a suitable method)(Table 9).ParasitologyRoutine methodology including faecal flotation.Serology for Toxoplasma gondii andTrichinella spiralis. Individual blood/serumsamples.No anthelmintic or ectoparasite treatmentshould have been undertaken within 10weeks before sampling.Table 10Monitoring of parasites (pig)Compulsory list of parasites to be monitored:All helminthsEimeria spp.Isosporaspp.Sarcoptes sp. (other arthropods when associated with lesions)Examples of parasites to be monitored on request:Cryptosporidium parvumEperythrozoon suisToxoplasma gondiiTrichinella(Ziehl-Neelsen staining, IFA)(serology HA)(serology)(serology)
FELASAWorkingGroup on Animal Health9Sampling time for parasitological examinationshould be immediately before retreatmentwith a parasiticide or when consistentwith the sanitary policy (Table 10).This document was compiled using thecombined expertise of the Working Groupand information contained in the followingkey references:Acha PN (1987) Zoonoses and Communicable DiseasesCommon to Man and Animals, 2nd edn.Washington, DC: Pan American Health OrganizationAppel MJ (1987) Virus Infections of Carnivores.Amsterdam: Elsevier Science Publishers BVBornstein S (1995) Sarcoptes scabiei Infections of theDomestic Dog, Red Fox and Pig. Clinical andSerodiagnostic Studies. Swedish University ofAgricultural Sciences, Sveriges Lantbruksuniversitet,UppsalaChandler EA, Gaskell q, Gaskell RM (1994) FelineMedicine and Therapeutics. Oxford: BlackwellScientific PublicationsEttinger S1, Feldman EC (19951 Textbook of VeterinaryInternal Medicine Diseases of the Dog andCat, 4th edn. Philadelphia: WB SaundersGaskell RM, Bennett M, Tenna B, Willoughby K(1996) Feline and Canine Infectious Diseases.Oxford: Blackwell ScienceGeorgi JR, Georgi ME (1992) Canine Clinical Parasitology.Philadelphia: Lea & FebigerGrant Dr (19911 Skin Diseases in the Dog and Cat,2nd edn. Oxford: Blackwell Scientific Library ofVeterinary PracticeGreene CE (19901 Infectious Diseases of the Dog andCat. Philadelphia: WB SaundersHamm TE Jr, ed. (1985) Complications of Viral andMycoplasmal Infection in Rodents to ToxicologyResearch and Testing. London: McGraw-HillKraft V, Deeny AA, Blanchet HM, Boot R, HansenAK, Hem A, van Herck H, Kunstyr I, Milite G,Needham JR, Nicklas W, Perrot A, Rehbinder C,Richard Y, De Vroey G (1994) Report of the FELASAWorking Group on Animal Health. Recommendationsfor the health monitoring of mouse, rat,hamster, guineapig and rabbit breeding colonies.Laboratory Animals 28, 1-12LABA/LASA Guidelines for the care of laboratoryanimals in transit (1992j Laboratory Animal BreedersAssociation of Great Britain Limited andLaboratory Animal Science Association. LaboratoryAnimals 27, 93-107Leman AD, Straw BE, Mengeling WC, D'Allaire S,Taylor DJ (1996) Diseases of Swine. Ames, Iowa:Iowa State University PressPensaert MB (1989) Virus Infections of Porcines.Amsterdam: Elsevier Science Publishers BVQuinn P1, Carter ME, Markey B, Carter GR (19941Clinical Veterinary Microbiology. London: Mosby-Wolfe PublishingRehbinder C, Hansen AK, eds (1993) The importanceof health monitoring in laboratory animals. ScandinavianTournaI of Laboratory Animal Science20(11 Special issue on health monitoringRehbinder C, Baneux P, Forbes D, van Herck H,Nicklas W, Rugaya Z, Winkler G (1996) FELASArecommendations for the health monitoring ofmouse, rat, hamster, gerbil, guineapig and rabbitexperimental units. Laboratory Animals 30, 193-208Sherding RG (19891 The Cat Diseases and ClinicalManagement, Vol 2. New York: Churchill LivingstoneSimpson JW, Else RW (19911 Digestive Disease in theDog and Cat. Oxford: Blackwell Scientific Libraryof Veterinary PracticeSpiegel A, Erichson S, Solleveld HA (1980) AnimalQuality and Models in Biomedical Research. 7thICLAS Symposium Utrecht (1979). Stuttgart, NewYork: Gustav FischerWallgren P (19931 Infections and Immune Functionsof Swine in Fattening Herds. Swedish University ofAgricultural Sciences. Sveriges lantbruksuniversitet,UppsalaWalton JR (1993) A Handbook of Pig Diseases, 3rdrev. edn. UK: Liverpool University PressWorking Committee for the biological characterizationof laboratory animals GV/SOLAS (1985)Guidelines for specification of animals and husbandrymethods when reporting the results ofanimal experiments. Laboratory Animals 19, 106-8
10FELASAWorkingGroup on Animal HealthFELASA-APPROVED HEALTH MONITORINGREPORTName and address of the breeder:Date of issue:Unit No:Currenttest date:Species: CatBreed:HISTORICALresultspos/testedCURRENT TESTresultspos/tested LABORATORY METHODVIRAL INFECTIONSFeline calicivirusFeline immunodeficiencyvirus (FIV)Feline infectious peritonitis virus(coronavirus, FIP)Feline intestinalFeline leukaemiacoronavirusvirus (FeLV)Feline parvovirusFeline rhinotracheitisvirusRotavirusVIRAL INFECTIONS TO BE MONITORED ON REQUEST
FELASAWorkingGroup on Animal Health11FELASA-APPROVED HEALTH MONITORING REPORTName and address of the breeder:Date of issue:Unit No:Currenttest date:Species: CatBreed:HISTORICALresultspos/testedCURRENT TESTresultspos/tested LABORATORY METHODBACTERIAL AND FUNGAL INFECTIONSBartonellaspp.BordetellabronchisepticaCampy/obacterspp.Chlamydiapsittac;Microsporumspp.PasteurellaceaeSalmonellaspp.Staphylococcus spp. (whenassociated with lesions)Streptococci betahaemolyticserogroup GTrichophyton spp.Yersinia enterocoliticaBACTERIAL AND FUNGAL INFECTIONSTO BE MONITORED ON REQUESTHelicobacterspp.
12FELASAWorkingGroup on Animal HealthFELASA-APPROVED HEALTH MONITORING REPORTName and address of the breeder:Date of issue:Unit No:Currenttest date:Species: CatBreed:HISTORICALresultspos/testedCURRENT TESTresultspos/tested LABORATORY METHODPARASITIC INFECTIONSAll arthropodsAll heminthsEperythrozoon felisHaemobartonella felisIsospora spp.SarcocystisToxoplasmagondi;PARASITIC INFECTIONS TO BE MONITORED ON REQUESTPATHOLOGICAL LESIONSOBSERVEDOrgan:Organ:Organ:Organ:Organ:Organ:Lesions:Lesions:Lesions:Lesions:Lesions:Lesions:ABBREVIATIONS FOR LABORATORIESStandard operating procedures can be obtained from --------------------
FELASAWorkingGroup on Animal Health13FELASA-APPROVED HEALTH MONITORINGREPORTName and address of the breeder:Date of issue:Unit No:Currenttest date:Species: DogBreed:HISTORICALresultspos/testedCURRENT TESTresultspos/tested LABORATORY METHODVIRAL INFECTIONSCanine adenovirusCanine distempertype 1 (HCC)virusCanine parainfluenzavirusCanine parvovirus(CPV)VIRAL INFECTIONSTO BE MONITORED ON REQUESTBACTERIAL AND FUNGAL INFECTIONSBordetellabronchisepticaBorreliaspp.Brucella canisLeptospiraSalmonellaspp.spp.Streptococciserogroupbeta-haemolytic,GBACTERIAL AND FUNGAL INFECTIONSTO BE MONITORED ON REQUEST
14FELASAWorkingGroup on Animal HealthFELASA-APPROVED HEALTH MONITORING REPORTName and address of the breeder:Date of issue:Unit No:Currenttest date:Species: DogBreed:HISTORICALresultspos/testedCURRENT TESTresultspas/tested LABORATORY METHODPARASITIC INFECTIONSAll arthropods(Demodex sp. only whenassociated with lesions)All helminthsCoccidiaeGiardia spp.HaemobartonellacanisPARASITIC INFECTIONS TO BE MONITORED ON REQUESTPATHOLOGICAL LESIONS OBSERVEDOrgan: ----- Lesions: ---------------------------Organ: ----- Lesions: ---------------------------Organ: ----- Lesions: ---------------------------Organ: ----- Lesions: ---------------------------ABBREVIATIONS FOR LABORATORIESStandard operating procedures can be obtained from
FELASAWorkingGroup on Animal Health1SFELASA-APPROVED HEALTH MONITORINGREPORTName and address of the breeder:Date of issue:Unit No:Currenttest date:Species: PigBreed:HISTORICALresultspos/testedCURRENT TESTresultspos/tested LABORATORY METHODVIRAL INFECTIONSAfricanswine feverAujeszky disease virus (pseudorabies) ------Classical swine feverEncephalomyocarditis(hog cholera)virusHaemagglutinatingencephalomyelitisPorcine cytomegalovirus(inclusion body rhinitis)Porcine epidemic diarrhoea (whenassociated with disease)Porcine influenza(H1N1, H3N2)Porcine parvovirusPorcine respiratorycoronavirusPorcine reproductive and respiratory ------syndrome (PRRS)Porcine rotavirusSMEDITeschen/TalfanvirusTransmissible gastroenteritis (TGE)VIRAL INFECTIONSTO BE MONITORED ON REQUESTAND WHEN PRESENTIN THE COUNTRY
16FElASA WorkingGroup on Animal HealthFELASA-APPROVED HEALTH MONITORING REPORTName and address of the breeder:Date of issue:Unit No:Currenttest date:Species: PigBreed:HISTORICALresultspos/testedCURRENT TESTresultspos/tested lABORATORY METHODBACTERIAL, MYCOPLASMALINFECTIONSActinobacilluspleuropneumoniaeBordetel/abronchisepticaErysipelothrixrhusiopathiaeEubacterium (Corynebacterium suis)HaemophilusparasuisleptospiraMycoplasmaspp.hyopneumoniaePasteurella mu/tocida(toxin producing)Salmonellaspp.StaphylococcushyicusStreptococciStreptococcusbeta-haemolyticsuisYersinia enterocofiticaBACTERIAL, MYCOPLASMALAND FUNGAL INFECTIONS TO BE MONITORED ON REQUEST
FELASAWorkingGroup on Animal Health17FELASA-APPROVED HEALTH MONITORING REPORTName and address of the breeder:Date of issue:Unit No:Currenttest date:Species: PigBreed:HISTORICALresultspas/testedLATEST TESTresultspos/tested LABORATORY METHODPARASITIC INFECTIONSAll helminthsEimeria spp.Isospora spp.Sarcoptes (other arthropods whenassociated with disease)PARASITIC INFECTIONS TO BE MONITORED ON REQUESTPATHOLOGICAL LESIONSOBSERVEDOrgan:Organ:Organ:Organ:Organ:Lesions:Lesions:Lesions:Lesions:Lesions:ABBREVIATIONS FOR LABORATORIESStandard operating procedures can be obtained from