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Enzymes and Enzyme Kinetics - CMBE

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C) V MAX ′ = 12 × 10 -4 mol/cm 2 s (S BULK = 7 mmol/cm 3 )Da = =V MAX′______k L S BULKK______ MS BULK=_____________(12 × 10 -4 )= 3.986(4.3 × 10 -5 )(7)=____(2)(7)= 0.2857 = Da + – 1 = 3.272_________ x = (-1 ± √1 + 4/2 ) = 0.0851S SURF = xS BULK = (0.0851)(7.0) = 0.60 mol/cm 3V TRUE =V_______ MAX ′x + x=________________(12 × 10 -4 )(0.0851)(0.2857 + 0.0851)= 2.75 × 10 -4 mol/cm 2 s________ x ( + 1) E = = + x________________(0.0851)(1.2857)(0.2857 + 0.0851)= 0.30

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