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BD LSR Fortessa Standard Operation Protocol --- Turn on and turn ...

BD LSR Fortessa Standard Operation Protocol --- Turn on and turn ...

BD LSR Fortessa Standard Operation Protocol --- Turn on and turn ...

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<str<strong>on</strong>g>BD</str<strong>on</strong>g> <str<strong>on</strong>g>LSR</str<strong>on</strong>g> <str<strong>on</strong>g>Fortessa</str<strong>on</strong>g> <str<strong>on</strong>g>St<strong>and</strong>ard</str<strong>on</strong>g> <str<strong>on</strong>g>Operati<strong>on</strong></str<strong>on</strong>g> <str<strong>on</strong>g>Protocol</str<strong>on</strong>g>--- Refill the sheath c<strong>on</strong>tainer• Verify that the flow cytometer is in st<strong>and</strong>by mode. Press the STNDBYbutt<strong>on</strong> <strong>on</strong> the c<strong>on</strong>trol panel if necessary.• Click “Cytometer” > “st<strong>and</strong>by” in Diva Software• Disc<strong>on</strong>nect the air line (green) <strong>and</strong> fluid line (Blue) from the sheathc<strong>on</strong>tainer.• Depressurize the sheath c<strong>on</strong>tainer by pulling up <strong>on</strong> the vent valve.• Remove the sheath c<strong>on</strong>tainer lid. Unscrew the clamp knob <strong>and</strong> pushdown to loosen, if necessary. Tilt the cap to the side to remove it fromthe tank.• Add sheath fluid to the sheath c<strong>on</strong>tainer (please use the blue bottle).• Replace the sheath c<strong>on</strong>tainer lid. Close the sheath lid <strong>and</strong> tighten theclamp knob to finger-tight.• Rec<strong>on</strong>nect the air line (green) <strong>and</strong> fluid line (Blue).Please pay attenti<strong>on</strong> to fluid line (Blue) to make sure it is not twisted orstretched.• Rec<strong>on</strong>nect Diva software with workstati<strong>on</strong> by click “Cytometer” >“C<strong>on</strong>nect”.• Remove air bubbles from the sheath filter.

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