Chrysanthemum - Protection of Plant Varieties & Farmers

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III. Conduct of Tests:1. The minimum duration of the test shall normally be one independent growing season withreference to the ecosystem of the variety submitted for DUS test. However, in case thematerial entered does not meet the DUS criteria for any one or more than one character, thenthe test shall be extended for the next year.2. Tests shall normally be conducted at one place. If any essential characteristics of the varietyare not expressed for visual observations at this place, the variety shall be considered forfurther examination at another appropriate test site or under special test protocol on expressedrequest of the applicant.3. The field tests shall be carried out under conditions favouring normal growth and expressionof all test characteristics. The size of the plots shall be such that plants or parts of plants couldbe removed for measurement and observation without prejudicing the other observations onthe standing plants until the end of the growing period.4. In particular, growth regulators should not be used.5. Varieties should be disbudded, if bred for such use, but where necessary, in the case of dualpurpose varieties, distinctiveness should also be checked on non-disbudded plants.IV. Methods and observations1. The characteristics described in the Table of characteristics shall be used for the testing ofvarieties for their DUS (section VII).2. Unless otherwise indicated, the optimum stage of development for the assessment of thecharacteristics is the time of full flowering.3. All observations on vegetative organs shall be recorded unless otherwise indicated on themiddle third of the stem. Colours of vegetative organs shall be observed on plants exposed tomaximum light.4. All observations on floral parts shall be made on terminal flower heads. The colour of the rayflorets shall, unless otherwise indicated be recorded at stage 8 on the scale of flower headdevelopment (Cockshull & Hughes, 1967 & 1972).5. Unless otherwise indicated, all observations on single plants shall be made on 20 plants orparts taken from each of 20 plants and any other observations made on all plants in the test.42
6. Each test shall include a total of at least 50 plants in two replications. For assessment ofDistinctiveness and Stability, all observations shall be made on 40 plants out of 50 plants. Outof the 40 plants, 20 plants shall be disbudded / pinched and the other 20 plants withoutdisbudding/pinching.7. For the assessment of Uniformity of vegetatively propagated varieties, a population standardof 1% and an acceptance probability of at least 95 % shall be applied. In the case of a samplesize of 20 plants, 1 off-type is allowed. In practice, it is not usual to perform tests of Stabilitythat produce results as certain as those of the testing of Distinctiveness and uniformity.However, experience has demonstrated that for many types of variety, when a variety hasbeen shown to be uniform, it can also be considered to be stable. Where appropriate or incases of doubt, stability may be tested either by growing a further generation or by testing anew plant stock to ensure that it exhibits the same characteristics as those shown by theprevious material supplied.8. For the assessment of colour characteristics, the latest Royal Horticultural Society (RHS)colour chart shall be used. Because daylight varies, colour determinations made against colourchart shall be made either in a suitable cabinet providing artificial daylight or in the middle ofthe day in a room without direct sunlight. The special distribution of illuminant for artificialdaylight should conform with the CIE Standard of Preferred daylight D 6500 and should fallwithin the tolerance set out in the British Standard 950, Part 1.The DUS test centre mustacquire them to ensure uniform observation. These determinations shall be made with theplant part placed against a white background.9. Additional tests protocols for special purpose shall be established by the PPV&FR Authority.10. Standard cultural practices to be adopted and specified as may be relevant to the location ofthe DUS test centres for open and greenhouse conditions. If however, any material entered forthe DUS test has any specific requirement for expression of characters, the same will bespecified by the applicant. The DUS test centres shall finalize the standard cultural practiceswith the approval of the Authority.V. Grouping of Varieties1. The candidate varieties for DUS testing shall be divided into groups to facilitate theassessment of Distinctiveness. Characteristics which are known from experience not to vary43
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Chrysanthemum - Protection of Plant Varieties & Farmers

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