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Slides - Situs

Slides - Situs

asymmetricreconstructionmost novel partsymmetry searchimposedsymmetry

Summary of steps in IHRSR procedure• Box stretches of filaments from EM• cut into overlapping segments of fixed length• optimal length dictated by disorder, Signal/Noise• extent of overlap generated by axial rise per subunit• Do initial alignment to center segments – avoid interpolation• Generate an initial reference – featureless cylinder works!• Make guess about approximate symmetry• Can use information from power spectrum, AP SR, 2 1 screw…• Azimuthal increment (number of reference projections) determined bydiameter of filament, resolution desired• Check convergence, check AP NQ statistics, check agreement betweeneenpower spectrum and Fourier Transform of reconstruction

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