RanselWhy measure Glutathione peroxidase (GPx) and selenium status?Selenium is an essential trace element, involved in the aetiology of several diseases. At normal concentrations, selenium mayhave a protective effect against several diseases, including cancer. However, this protection is lost at low concentrations andselenium is highly toxic at high concentrations. Therefore, it is important that selenium levels should be monitored, so that theymay be kept within the normal range. Ransel measures glutathione peroxidase which has a direct correlation with seleniumlevels.Pharma• Determination of the therapeuticefficacy and antioxidant potential ofdrugs.RanselApplicationsHow does RANSEL work?Ransel shows excellent correlationwith the original method of GPxassessment developed by Pagliaand Valentine 1 . The method is basedon the reaction below:2GSH + ROOHGPxGSSG + ROH + H 2OGSSG + NADPH + H + glutathione reductase 2GSH + NADP +(ROOH = cumene hydroperoxide)Clinical & veterinary• Patients or animals suffering fromdiseases related to GPx or seleniumdeficiency.• Identification of those with increasedrisk of selenium deficiency - riskfactors include increasing age, poornutritional status, smoking, alcoholism,stress, renal failure, crohn’s disease,cystic fibrosis, autoimmune diseaseand chemotherapy.• Identification of GPx and seleniumdeficiency in patients allows dietarysupplementation of selenium andantioxidants.The concentration of GPx isassessed from the decrease inabsorption at 340 nm due to theoxidation of NADPH to NADP +GPx - RBC (U/g Hb)relationship betweenGPx activity and plasmaselenium concentration40302010DeficiencyNormalPLATEAUToxic60 80 10 10 200Plasma Selenium (ug/l)1. Paglia, D.E. and Valentine, W.N., J. Lab. Clin. Med., 1967; 70: 158.Sports• Identifying and correctly treatingsports professionals at risk of seleniumdeficiency.Did you know?Free radicals can come fromenvironmental pollution,radiation, cigarette smoke,chemicals, and herbicides.
Glutathione ReductaseGlutathione reductase is required for the regeneration of reduced glutathione which is important for normal cellular metabolism.This enzyme is often discussed in association with glutathione peroxidase, which requires reduced glutathione for activation.Why measure Glutathione Reductase?Glutathione reductase is responsible for maintaining levels of reduced glutathione which has many important functions in thecell. Glutathione plays a role in protein folding and the maintenance of reduced pools of vitamin C and E. Reduced levels of thisenzyme have been described in several diseases.Glutathione ReductaseApplicationsSports• Assessing enzyme deficiency statesand cell metabolism.• Assessment of nutrition(riboflavin status).How does the glutathionereductase assay work?Glutathione reductase catalyses thereduction of glutathione (GSSG) inthe presence of NADPH, which isoxidised to NADP + .GSSG + NADPH + H + glutathione reductase 2GSH + NADP +GSH = Reduced glutathioneGlutathione Facts:• Did you know glutathione is themost powerful, prevalent antioxidantin your body?• Powerful regenerator of immunecellsClinical & veterinary• Assessing enzyme deficiency statesand cell metabolism.• Detection of hepatic and malignantdisease.• Detection of genetically determineddeficiency states.• As a research tool, to help identifydiseases in which free radicals areinvolved.• The most important agent inkeeping the body detoxified andhealthy.• Vital in the synthesis of DNA andmaintenance of DNA.• Vital in the synthesis of protein andamino acids.• Strong antiviral effects.examples of applications using randox antioxidant products• Vinson, J.A., et al. (2001) Meganatural ((R)) gold grapeseed extract: in vitro antioxidant and in vivo humansupplementation studies. J.Med. Food. 4: 17-26. (TAS)• Coyado Bispo, (2008), Measuring the Antioxidant Potential of an Açaí Extract Cosmetics & Toiletries magazineVol. 123 (TAS) 123:47-50• Simonetti, P., et al. (1997) Polyphenol content and total antioxidant potential of selected Italian wines. J. Agric. food.chem, 45: 1152-1155. (TAS)• Pelle, E., et al. (2002) A test for antioxidant activity in cosmetic formulations. J. Cosmet. Sci., 53: 237-240. (TAS)• Walsh, D.M., et al. (1993) Vitamin E and selenium deficiencies increase indices of lipid peroxidation in muscle tissueof ruminant calves. Int. J. of Vitam. Nutr. Res 63. 188-194. (RANSEL)• Sitarska et al. (1997) Antioxidant system in horses and attempts for its modulation. Medycyna Wet., 53(10). (TAS,RANSOD, RANSEL)
“Revolutionising healthcare through continuously improving diagnostic solutions”Randox Laboratories Ltd., 55 Diamond Road, Crumlin, Co. Antrim, BT29 4QY, United Kingdomt: +44 (0) 28 9442 2413 f: +44 (0) 28 9445 2912 e: firstname.lastname@example.org www.randox.comRandox Laboratories Limited is a company registered within Northern Ireland with company number N.I. 15738. VAT Registered Number: GB 353 030 400Product variability may vary from country to country. Please contact your local Randox representative for information.May 2009LT086