10th International Magnesium Symposium Schedule of Events

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9 Molecular biology of magnesium transport proteins Matin Piskacek*, Jochen Stadler*, Martin Kolisek*, Gabor Zsurka*, Julian Weghuber*, Monika Schweigel**, Rudolf J. Schweyen*. * Vienna Biocenter, Institute of Microbiology and Genetics, University of Vienna, Dr. Bohrgasse, A-1030 Vienna, Austria; ** Free University Berlin, Institute of Veterinary-Physiology, Oertzenweg 19b, D-14163 Berlin, Germany. rudolf.schweyen@univie.ac.at We have recently characterized a mitochondrial protein (named Mrs2p) as constituting the major mitochondrial Mg2+ transport protein in the yeast Saccharomyces cerevisiae. Combined physiology and molecular studies suggest that it forms a channel in the inner mitochondrial membrane mediating high capacity Mg2+ influx, driven by the inside negative membrane potential. This protein is a distant homologue of the bacterial CorA Mg2+ transport protein and it has well conserved homologues in all eukaryotes. The human genome encodes a single Mrs2 homologue. Work is in progress to characterize its role in Mg2+ influx into mitochondrial DNA by using cell cultures overexpressing hsMrs2p and cell cultures with knock-down of its mRNA.
10 Mg2+ transport proteins of the yeast plasma membrane and cytoplasmic homeostasis factors Jochen Stadler, Anton Graschopf, Stefan Köstler, Rudolf J, Schweyen. Max Perutz Laboratories, Department of Microbiology and Genetics / University of Vienna, Campus Vienna Biocenter Dr. Bohrgasse 9, A-1030 Vienna, Austria. rudolf.schweyen@univie.ac.at The major Mg2+ influx into yeast cells is mediated by Alr1p, an integral protein of the plasma membrane. Alr1p is a distant relative of the bacterial CorAp and mitochondrial Mrs2p Mg2+ transport proteins. Like these proteins, Alr1p appears to form oligomeric complexes in the plasma membrane, which probably constitute Mg2+ channels. Regulation of Mg2+ fluxes into the cell is mediated by transcriptional control of the ALR1 gene and, more importantly, by Alr1p endocytosis and degradation in the vacuole. A novel factor, Cos16p, has been identified as being an integral protein of the endoplasmic reticulum (ER) and essential for tolerance of yeast cells to elevated extracellular Mg2+ concentrations. Unlike Alr1p, which occurs in lower eukaryotes only, Cos16p is well conserved from yeast to man. Its overexpression or absence in yeast cells causes a decrease or increase, respectively of total cellular Mg2+ concentrations. We speculate on the involvement of Cos16p in the Mg2+ exchange between the endoplasmic reticulum and the cytoplasm. As revealed from whole genome expression profiling, Mg2+ starvation of yeast cells elicits a highly significant upshift of gene activities by the calcineurin pathway, similar to Ca2+ overload. Work is in progress to learn which regulatory mechanisms are involved in this response.
Page 1 and 2: INTERNATIONAL SOCIETY FOR THE DEVEL
Page 3 and 4: Schedule of Events
Page 5 and 6: Session 3 GENETICS Chair: Dr Richar
Page 7 and 8: WEDNESDAY, SEPTEMBER 10 TH Session
Page 9 and 10: THURSDAY, SEPTEMBER 11 TH Session 1
Page 11 and 12: Magnesium in asthma attack G. Sur,
Page 13 and 14: 2 Mental benefits of estrogen repla
Page 15 and 16: 4 Effects of magnesium on blood-bra
Page 17 and 18: 6 Effect of magnesium on neural act
Page 19: 8 Magnesium transport genes in yeas
Page 23 and 24: 12 Interest of stable isotopes use
Page 25 and 26: 14 Depletion of intracellular Mg2+
Page 27 and 28: 16 Magnesium and the inflammatory r
Page 29 and 30: 18 The effect of magnesium deficien
Page 31 and 32: 20 Magnesium attenuates post-trauma
Page 33 and 34: 22 Effects of Oral Magnesium Therap
Page 35 and 36: 24 Optimal administration dosage of
Page 37 and 38: 26 Intracellular magnesium assay co
Page 39 and 40: 28 Long-Term Outcome of Intravenous
Page 41 and 42: 30 Case for subcutaneous magnesium
Page 43 and 44: 32 Functional effects of magnesium
Page 45 and 46: 34 Cardiovascular and skeletal bene
Page 47 and 48: 36 Bone mineral density, serum albu
Page 49 and 50: 38 Magnesium in sports Frank C. Moo
Page 51 and 52: 40 A functional biological marker i
Page 53 and 54: 42 Balance of Mg positively correla
Page 55 and 56: 44 Magnesium and cancer in clinical
Page 57 and 58: 46 Magnesium, insulin resistance an
Page 59 and 60: 48 Post-cholecystectomy syndrome an
Page 61 and 62: 50 A substance P antagonist increas
Page 63 and 64: 52 Propofol attenuates the neuropro
Page 65 and 66: 54 Determination of extracellular m
Page 67 and 68: 56 Contribution of the Na+-Mg2+ exc
Page 69 and 70: 58 Modulation of tyrosine kinases a
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60 Effects of reduced magnesium ava
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62 Serum magnesium levels and depen
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64 About the misdiagnostics of magn
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66 Experimentally induced prolonged
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68 Lyme disease and magnesium defic
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70 Magnesium in animal nutrition Ka
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10th International Magnesium Symposium Schedule of Events

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