First Report of Gray Mold on Crepe Myrtle (Lagerstroemia indica ) Caused by Botrytis cinerea in Korea

Welcome Sign in | Register | Mobile
Journals Home Books Home APS Home IS-MPMI Home My Pr<strong>of</strong>ile Subscribe Search Advanced Search Help
Share
About the current
issue's cover
Impact Factor: 3.02
ISSN: 0191-2917
SEARCH
Enter Keywords
Phytopathology
Plant Disease
MPMI
Editor-in-Chief: Alison E. Robertson
Published by The American Phytopathological Society
Home > Plant Disease > Table <strong>of</strong> Contents > Abstract
Previous Article | Next Article
Accepted for publication
http://dx.doi.org/10.1094/PDIS-12-15-1521-PDN
<strong>First</strong> <strong>Report</strong> <strong>of</strong> <strong>Gray</strong> <strong>Mold</strong> on Crepe Myrtle
(Lagerstroemia indica ) Caused by Botrytis cinerea
in Korea
Quick Links
Add to favorites
E-mail to a colleague
Alert me when new articles
cite this article
Download to citation
manager
Related articles
found in APS Journals
Advanced Search
Resources
Subscribe
About Plant Disease
<strong>First</strong> Look
Most Downloaded
Articles
Submit a Manuscript
Customer Care
About My Password
Copyright and
Permissions
Plagiarism and Ethics
Advertise
Open Access
ORCID Registry
Mrs. Tania Afroz
Gangneung-Wonju National University, Department <strong>of</strong> Plant Science , 7, Jukheon-gil,
Gangneung, Korea, Republic <strong>of</strong>; tulyplp@gmail.com
Mr. Md Aktaruzzaman
Gangneung-Wonju National University, Department <strong>of</strong> Plant Science , 7 Jukheon-gil,
Gangneung, Gangwon-Do, Korea, Republic <strong>of</strong>, 210-702; jewellhstu.plp@gmail.com
Dr. Byung-Sup Kim, Ph D
Gangneung-Wonju National University, Department <strong>of</strong> Plant Science , 7 Jukheon-gil,
Gangneung-si, Gangneung, Korea, Republic <strong>of</strong>, 25457, +82-33-640-2353, +82-33-640-
2909; bskim@gwnu.ac.kr
Dr. Gab-soo Han, Ph.D
Gangneung-Wonju National University College <strong>of</strong> Life Sciences, 294554, Environmental
Landscape Architecture, 7 Jukheon-gil, Gangneung, Gangwon, Korea (the Republic <strong>of</strong>) , 210-
702, +82-33-640-2477, +82_33-640-2359; hangs@gwnu.ac.kr
Dr. Hyeon-Dong Shin
Korea University, Environmental Science & Ecological Engineering , Seoul, Seoul, Korea,
Republic <strong>of</strong>, 02841, 82-2-3290-3063, 82-2-921-1715; hdshin@korea.ac.kr
PDF Print | PDF with Links
Crepe myrtle (Lagerstroemia indica, Lythraceae) is a flowering shrub widely planted in
warmer climate zones as landscape plant, generally producing diverse colored flowers in
summer and early fall (Qin and Graham 2007). In Korea, gray mold disease was first
observed during the rainy season in 2013 in Gangneung, Korea. In 2014 the disease was not
aggressive, but in July 2015 approximately 5 to 10% <strong>of</strong> inflorescences displayed symptoms
<strong>of</strong> gray mold that reduced the aesthetic value <strong>of</strong> flowers. Water-soaked, brown or gray spots
followed by abundant mycelia with conidia appeared on the affected inflorescences. Diseased
tissue was surface-sterilized by immersing in 0.1% sodium hypochlorite (NaOCl) for 1 min,
rinsed three times with sterile water, placed on potato dextrose agar (PDA, Difco), and
incubated at 20 ± 2°C. The fungus formed black sclerotia ranging from 1.1 to 4.4 × 1.0 to
3.2 mm (n = 20) near the edges <strong>of</strong> culture dishes. Conidia ( n = 50) were ellipsoidal or ovoid,
5.6 to 8.1 × 5.7 to 9.6 µm on naturally infected inflorescences and 5.8 to 10.6 × 5.3 to 7.5
µm on PDA. Morphological characteristics were consistent with those <strong>of</strong> Botrytis cinerea (Ellis
1971). The internal transcribed spacer (ITS) region <strong>of</strong> rDNA <strong>of</strong> the Korean isolate BR003 was
amplified with universal primers ITS1/ITS4 and sequenced. BLAST analysis <strong>of</strong> the resulting
528-bp nucleotide ITS segment (GenBank, KT728901) showed 99% similarity with the
sequence <strong>of</strong> Botryotinia fuckeliana (teleomorph <strong>of</strong> B. cinerea). Since the ITS region is not
informative for identifying Botrytis isolates to the species level, the glyceraldehyde-3-
phosphate dehydrogenase (G3PDH), heat-shock protein 60 ( HSP60), and DNA-dependent
RNA polymerase subunit II (RPB2) that are commonly used for Botrytis species

RNA polymerase subunit II (RPB2) that are commonly used for Botrytis species
identifications (Staats et al. 2005), were also sequenced for isolate BR003. The G3PDH,
HSP60, and RPB2 sequences <strong>of</strong> BR003 (KT728902, KT728903, and KT728904) had 99, 100
and 99% identity with the published GenBank accessions KM016535, KM016534 and
KM032181 respectively. For pathogenicity testing, three detached inflorescences were
sprayed with a conidial suspension (2 × 10 4 conidia/ml) prepared from a PDA culture and
were maintained with petioles immersed in sterilized water. An analogous three
inflorescences, serving as controls, were sprayed with sterilized water. All inflorescences were
sealed in plastic bags and incubated in humid chambers at 20 ± 2°C. After 5 days flower
petals <strong>of</strong> the inoculated treatments had brownish blemishes and blackish gray mycelia with
conidia, whereas control inflorescences remained symptomless. The pathogenicity test was
repeated with similar results. The pathogen was successfully re-isolated from inoculated
flowers, fulfilling Koch’s postulates. <strong>Gray</strong> mold disease <strong>of</strong> L. indica caused by B. cinerea has
been recorded in China and New Zealand (Farr and Rossman 2015). To our knowledge, this is
the first report <strong>of</strong> gray mold on L. indica in Korea. According to our field observations, gray
mold was active at low temperatures and lesions expanded rapidly in humid conditions.
Journals Home Books Home APS Home IS-MPMI Home Contact Us Permissions Privacy Copyright The American Phytopathological Society

<strong>Gray</strong> mold caused by Botrytis cinerea on crepe myrtle (Lagerstroemia indica). A,
Inflorescence damaged by gray mold. B, <strong>Gray</strong> mold developed 5 days after artificial
inoculation. C, Two-week-old colony <strong>of</strong> B. cinerea on PDA. D, Black sclerotia
developed on PDA. E. Conidia. 118x127mm (300 x 300 DPI)