Table of Contents - WOC 2012

PO-GLA-07
Comparison of Optic Nerve Head Parameters Using Heidelberg
Retinal Tomography 3 and Spectral-Domain Optical Coherence
Tomography
Hirooka Kazuyuki (1) , Sato Shino (1) , Baba Tetsuya (1) , Shiraga Fumio (1)
1. Kagawa University Faculty of Medicine
Purpose: To assess the agreement between the Heidelberg Retina Tomograph
(HRT) and Cirrus spectral-domain OCT (Cirrus HD-OCT) when measuring
optic disc parameters.
Methods: A total of 56 glaucoma patients and 21 normal subjects were
analyzed. Optic nerve head (ONH) measurements including disc area, rim
area, cup-to-disc ratio, and cup volume were obtained using both the Cirrus
HD-OCT and HRT 3. Bland-Altman plots were used to evaluate the agreement
between each of the optic disc parameters.
Results: While Cirrus HD-OCT values were smaller than HRT 3 values for the
disc and rim areas, overall the Cirrus HD-OCT and HRT 3 measurements
were highly correlated (r = 0.668 to 0.820). As compared to HRT 3, disc and
rim areas tended to be minimized by Cirrus HD-OCT, although there was no
relationship between the discrepancy and the level of measurement of the cupto-disc
ratio.
Conclusion: Although Cirrus HD-OCT and HRT 3 ONH analysis are highly
correlated, Bland-Altman analyses revealed that with the exception of the cupto-disc
ratio, there was poor agreement between the Cirrus HD-OCT and HRT
3 morphometric measurements. The ONH measurements could not be directly
compared between the Cirrus HD-OCT and HRT 3.
PO-GLA-08
Changes of mitochondria in trabecular meshwork cells of glaucoma
patients
Kuroyedov Alexander (1) , Ogorodnikova Victoria (1) , Gorodnichiy Vitaliy (1) ,
Smirnova Elena (2)
1. Mandryka 2nd Central Clinical Military Hospital
2. Russian N.N. Blokhin Cancer Research Center
Purpose. To study the state of mitochondria and apoptosis in cells of trabecular
meshwork (TM) of glaucoma patients. Methods. 40 glaucoma patients (40
eyes) were included. All patients underwent nonpenetrating filtering surgery.
Specimens of TM were separated and investigated by electron microscope.
Results. The sings of generation of TM were revealed: the depression of
intercellular space, sealing the contact zones between longitudinal and
transverse collagen fibers, a significant content of pigment granules in the
cytoplasm. The marginal consolidation of nuclear chromatin consolidation, as
sign of initial phase of apoptosis dominate in advanced stage of glaucoma.
Nuclei fragmentation and few mitochondria were found in advanced stage of
glaucoma. Conclusion. The results indicate the progression of the disease
is accompanied by mitochondria degeneration in the trabecular meshwork,
followed by the triggering of apoptosis. Ultrastructural analysis makes it
possible to determine the degree of change in the mitochondria and apoptosis
development. The damage of mitochondria is correlated with the stage of
glaucoma.
WOC2012 Abstract Book
PO-GLA-09
The Protective Effect of Ginkgo Biloba Extract on Cultured Retinal
Ganglion Cells
Kawase Kazuhide (1) , Moroto Naoya (1) , Yamamoto Tetsuya (1) , Ogawa Kenjirou
(2) , Hara Hideaki (2)
1. Department of Ophthalmology Gifu University School of Medicine
2. Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceuti
Purpose Ginkgo Biloba Extract (GBE) is used for the treatment of glaucoma. In
this study, we investigated whether GBE shows neuroprotective effects against
retinal damage on cultured retinal ganglion cells-5 (RGC-5) and exhibits free
radical scavenging activities. Methods RGC-5 cells were inoculated, 2×103
cells per well in 96-well plates. The GBE (3, 10, 30 ?g/ml), edaravone (10
?g/ml) and trolox (10 ?g/ml) as positive control were added in each well 1
hour before H2O2-induced oxidative stress. We observed the effects of each
compound on cell viability by using combination staining with two fluorescent
dyes, Hoechst 33342 and PI after 27 hours H2O2 administration. The free
radical scavenging activities of each compound were measured by electron
spin resonance (ESR) to evaluate the 2,2-diphenyl-1-picrylhydrazyl (DPPH)
radical scavenging activity. Results GBE reduced the H2O2-induced RGC-
5 cells damage in a concentration-dependent manner. Furthermore, GBE,
Edaravone and Trolox exhibited potent free radical scavenging activity
against DPPH radical, with IC50 values of 31.6?g/ml, 2.7?g/ml and 3.4?g/ml
respectively. ConclusionThese findings suggest that GBE may be a candidate
for effective treatment of retinal ganglion cell damage such as in glaucoma.
PO-GLA-10
Characteristics of patients with a localized retinal nerve fiber layer
defect and normal optic disc appearance
Lee Jeongmin (1) , Kim Junglim (2) , Kee Changwon (1)
1. Samsung Medical Center, Sungkyunkwan University School of Medicine
2. Pusan Paik Hospital, Inje University School of Medicine
PURPOSE: To investigate the characteristics of patients with a localized retinal
nerve fiber layer (RNFL) defect and normal optic disc.
Methods: Forty patients with an unchanged RNFL defect for more than three
years and normal optic disc were enrolled (Group A). We recruited 40 agematched
patients with normal tension glaucoma (Group B). On RNFL
photographs, angle ? and ? were the angles made by the line “L” from fovea
to disc and the lines “A” and “B” from the disc center to the disc margin where
the proximal and the distal border of the defect met, respectively. Line “R”
described two-thirds of the angular width of the defect. Angle ?2 was the angle
made by “L” and “R”. We compared systemic diseases, IOP and the angles
between the 2 groups.
Results: The percentage of systemic diseases and superotemporal RNFL
defects were greater in group A (P