R1-B

FastQC Report
R1 Sickle
Summary
• Basic Statistics
• Per base sequence quality
• Per tile sequence quality
• Per sequence quality scores
• Per base sequence content
• Per sequence GC content
• Per base N content
• Sequence Length Distribution
• Sequence Duplication Levels
• Overrepresented sequences
• Adapter Content
• Kmer Content

Basic Statistics
Measure
Value
Filename
R1_sickle.fastq
File type
Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 1828718
Sequences flagged as poor quality 0
Sequence length 20-300
%GC 64

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences
No overrepresented sequences

Adapter Content

Kmer Content

Sequence Count PValue Obs/Exp Max Max Obs/Exp Position
CTATTAA 35 4.323266E-4 105.74011 1
CTAGGAA 80 8.670151E-4 88.756256 250-254
CTATAGT 95 4.4410917E-6 64.92814 4
ACTAGAC 110 1.0592181E-5 56.0743 3
GTATAAG 185 4.8609345E-6 40.00977 2
TATAAGG 170 1.3843877E-4 36.28337 3
GTCTAAG 205 9.832156E-6 36.106377 1
CACTAGA 205 9.832156E-6 36.106377 2
TCCTAGA 265 1.5213645E-6 32.586575 2
TATAGGA 155 0.003849887 31.835732 2
CTAAGAC 165 0.005227392 29.90629 3
TACTATA 165 0.005227392 29.90629 2
CTAGACC 250 3.8205744E-5 29.60723 4
GCTCTAG 250 3.8205744E-5 29.60723 1
CTACACA 595 0.0 29.026695 4
ATACTAT 225 7.163306E-4 27.4141 1
CTAAAAC 275 7.314776E-5 26.91566 3
GGTATAA 230 8.144829E-4 26.818142 1
ATATAGG 185 0.0091385525 26.67318 1
CTCCTAG 280 8.2688144E-5 26.435028 1
Produced by FastQC (version 0.11.5)