CHARACTERIZING THE ROLES OF CELL POLARITY GENES ...
CHARACTERIZING THE ROLES OF CELL POLARITY GENES: PARD3, LIN7, AND SCRIBBLE IN ZEBRAFISH RETINA DEVELOPMENT A Dissertation Submitted to the Graduate School of the University of Notre Dame in Partial Requirement of the Requirements for the Degree of Doctor of Philosophy by Yiying Luo Graduate Program in Biology Notre Dame, Indiana December 2007 David R. Hyde, Director
the Morph-1 could efficiently knock
Fig 3.2: Pard3 expression in the re
domain (256-335) and the SH3 domain
Pard3 protein in the retinal neuroe
Fig 3.4: Immunoblot of Lin7 express
Fig 3.5: Immunolocalization of Lin7
Fig 3.6: Lin7 localizes to the gang
etinal neuroepithelium and mature r
3.3 Basalateral protein Scribble 3.
Fig 3.9: Scrib protein sequence ali
3.3.2 Expression pattern of Scrib p
protein expression, while the contr
cells, cells in the INL, IPL, OPL,
Morpholino Designation S79 S132 S13
Fig 3.12: The scrib morphant displa
3.23). Further experiments with dif
To assess the degree of the rescue,
Based on the presence or absence of
Fig 3.15: scrib mRNA injection resc
used to rescue the scrib morphant p
Fig 3.17: Comparison of Scrib prote
Fig 3.18: Immunoblots of wild-type
Fig 3.19: RT-PCR of the SE1I1 and S
Fig 3.20: Rescue S3 morphant phenot
Fig 3.21: Loss of the Scrib functio
epithelium like the severe scrib mo
Fig 3.22: Loss of Scrib function re
Fig 3.23: Loss of the Scrib functio
likely that these cells failed to e
To independently examine cyclin gen
3.3.10 Massive cell death in the sc
activity of the basalateral Scrib p
115
Fig 3.28: Expansion of apical subdo
Fig 3.29: Numb localization in wild
Fig 3.30: Beta-catenin localization
domains of epithelial cells (Wodarz
et al. 2004). The maintenance of th
4.3 Zebrafish Pard3 protein Besides
egions of the scrib gene sequence r
y the anti-Scrib antiserum. However
y down-regulating the expression of
experiments on the shh signaling pa
Bilder, D., M. Schober and N. Perri
Hurd, T. W., L. Gao, M. H. Roh, I.
Amano and S. Ohno (2003). "Self-ass
neurulation in Danio rerio." Dev Dy
Wada, H., M. Iwasaki, T. Sato, I. M
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