APPLICATION, CHALLENGES, AND PROSPECTS OF DAYLILY TISSUE CULTURE FOR GENETIC IMPROVEMENT The cloning and subsequent transfer into plants of ubiquitin inhibitor gene(s) and perhaps other genes including those influencing flower color and forms, might prolong the life and diversity of the color and shape of the flowers. In addition, the use of modern techniques might also avoid the incorporation of unwanted genes into the crop, which could occur during conventional plant breeding. MERISTEM AND INFLORESCENCE EXPLANTS AND RELATED CHALLENGES In daylily, methods for in vitro organogenesis have been established (Aziz et al., 2003; Krikorian and Kann, 1981; Meyer, 1976). The vegetative meristem can be used as explant of choice for in vitro plant regeneration (Smith and Krekorian, 1991). However, since it is located underground, meristematic tissues are difficult to obtain in a sterile condition and excision destroys the mother plant (Meyer, 1976). Of all the floral tissues investigated, the use of the whole inflorescence has become common in part because it is available en masse and less difficult to obtain in sterile condition. Also, it is relatively easier to manipulate the whole inflorescence than petals or sepals. However, limitations with the use of floral tissues are obvious. For instance, those tissues are not available all year-round and, further, they are reproductive cycle dependent. Thus, this limits the freedom of scientists working with daylily to plan and effectively carry out experiments when needed. LEAF EXPLANT POTENTIAL Leaf tissue may be the key to overcome such problems because: (1) with some exceptions, it is the only daylily organ above-ground that is available year-round; (2) it might be easier to obtain such tissues in sterile condition; (3) the use of the leaf as an explant might not affect the survival of the mother plant; and (4) the fact that leaf is flat and flexible might make it a more suitable target for particle bombardment and gene transfer, than the round and tough inflorescence. Further, it might be much easier to produce leaf discs providing an efficient wound area for Agrobacterium infection than the inflorescence for gene transformation. Despite all these advantages, the use of leaf tissue in daylily tissue culture is limited. This tissue is flexible and has successfully been used to produce protoplasts for subsequent production of callus and shoots (Ling and Sauve, 1995). However, the induction of shoots (Matand, 1999) or callus directly from leaf tissue has not been widely investigated (Matand, 1999). MORPHOGENIC POTENCY OF SELECTED GROWTH REGULATORS Morphogenesis in daylily has been manipulated using mostly common types of growth regulators such as NAA (naphthaleneacetic acid), 2, 4-D (2, 4-dichlorophenylacetic acid), BA (6-benzyl aminopurine), and KIN (kinetin), (Aziz et al., 2003; (Griesbach, 1989; Meyer, 1976). Thidiazuron (TDZ) has been proven to be a very powerful cytokinin. It is up to 10,000 times more active than DPU (N, N’-diphenylurea), and 10 times more active than 6.3 The Concept of Genetic Manipulation 189
zeatin (Pierik, 1987). In general, thidiazuron is 1000 times more active than other standard growth regulators (Huettman and Preece, 1993). The use of TDZ in daylily is also limited (Matand, 1999, Aziz et al., 2003) but has several advantages. TDZ is characterized by a unique rapid induction of repetitive multiple shoots across explants and plant species, which needs to be fully explored also in daylily. This chemical has consistently induced shoots faster in higher frequencies than other cytokinins commonly used. TDZ is successfully used across species to induce direct as well as indirect adventitious shoots (Matand and Prakash, 2007). It is used for shoot regeneration even at lower concentrations. The lowest TDZ recommended concentrations (established after investigation) in plant propagation is 10 8 M (Huetteman and Preece, 1993). TDZ concentrations lower than the preceding proposed is also applicable. Reports also suggest that when TDZ is being used for the first time in a new species, the initial experiment should be designed using 10 7 M as a middle concentration and evaluating two orders of magnitude above and below that concentration. The normally recommended concentration range of TDZ activity is 10 9 to 10 5 M (Huetteman and Preece, 1993). However, depending on the species, this range might proliferate more calli than shoots. Thus to reduce callus proliferation in favor of shoot growth, it is recommended to use concentrations up to 10 -4 M (Huetteman and Preece, 1993). PHYSICAL STATEOFTHEMEDIUM In some plant species, the success or failure of tissue culture might depend upon whether a liquid or an agar medium is employed (Murashige, 1974). For, instance, most bromeliads studied in laboratories could be started in cultures only in a liquid nutrient (Murashige, 1974). This has been true also with the cattleman orchid (Murashige, 1974). In contrast, shoot tip cultures of Asparagus (the closest relative of daylily) and Gerbera required initiation on agar gel medium (Chen and Galston, 1967). It has also been reported that the same species may require a different physical form of medium during each of the three growth stages in vitro. This change of the physical state of the nutrient medium in progressive stages of culture has been illustrated with Asparagus officinalis and Daucus carota (Murashige, 1974). In daylily, Krihorian and Kann (1981) demonstrated that besides semi-solid, liquid medium could be used to produce plants. Their studies suggested that the cultural conditions might be cultivar specific. They reported also that it is possible to use both solid and liquid medium to induce new shoots in daylily. Liquid medium was preferred over solid medium because of its more efficient use of space and the enhancement in shoot production (Krihorian and Kann, 1981). Also liquid medium has the capacity of diluting plant cell-excreted chemical toxic waste compared to solid medium. In liquid, explant cells are fully exposed to nutrients than on solid medium. However, their report failed to determine a shoot production ratio of liquid to solid medium in daylily. A rationale in the use of either physical medium needs further investigation in crop. 190 Chapter 6 Breeding Horticultural Plants THE INFLUENCE OF TRANSFORMATION METHOD IN REGENERATION EXPLANT SELECTION As indicated earlier, the selection of tissue culture method one should utilize for modern gene transfer into daylily should be dictated by the transformation technique. For instance, callus cells are more suitable for particle bombardment because a large number of cells that might be susceptible to subsequent plant regeneration can be efficiently arranged on the target plate for efficient gene blasts. This is also true for leaf tissue, except that in most cases plant regeneration is more likely to occur at leaf wound sites than non-wound areas. However, practically leaf wound cells are less likely to be
HORTICULTURE Principles and Practic
HORTICULTURE Principles and Practic
With love to Theresa, quarterback;
Brief Contents Preface xxi PART 1 T
Contents Preface xxi PART 1 THE UND
5.3 PLANT GROWTH PROCESSES 160 5.4
8.20 COMMON GREENHOUSE DISEASES 276
12.3 INTERNAL ENVIRONMENTAL CONTROL
PART 6 Summary 541 References and S
22.18 INDOOR COMPOSTING SYSTEMS 668
Preface Horticulture is the area of
ACKNOWLEDGMENTS I am very grateful
PART 1 THE UNDERLYING SCIENCE CHAPT
1 What Is Horticulture? PURPOSE AND
(a) (c) (b) (d) FIGURE 1-1 The many
FIGURE 1 Bridge. The plaza view of
CYCADS Many people mistake these pr
FIGURE 2 The world's largest unbran
FIGURE 2 Sold flowers are loaded on
FIGURE 1-4 Formal landscaping featu
1.4 ROLEOFTHENURSERY AND SEED INDUS
1.5 HORTICULTURE AND SOCIETY Hortic
TABLE 1-3 U.S. Horticultural Export
Turfgrass Operation 1. Landscape te
What Is Horticulture? This site pro
Examples of botanical gardens http:
2 Classifying and Naming Horticultu
Eight major taxa are commonly used
TABLE 2-3 The Divisions of the King
HISTORY OF PLANT TAXONOMY PAUL R. F
AGE OF HERBALISTS Two major events
possible system of nomenclature. Ho
TABLE 1 Type Categories for Plant N
2.3 OTHER CLASSIFICATION SYSTEMS (O
2. Shrubs. A shrub has no main trun
Simple Fruits Fleshy Fruits Drupe B
FIGURE 2-14 A pome, represented by
2.3.5 CLASSIFICATION OF VEGETABLES
(a) (b) FIGURE 2-22 (Source: George
FIGURE 2-25 A narrowleaf plant. (So
FIGURE 2-29 Parts of a typical gras
such as rosemary, sage, thyme, marj
c. Leaves d. Bulbs 2. Cut across (t
Whole plant Organs FIGURE 3-1 Level
ibonucleic acid (RNA), proteins, an
called cristae; this extreme foldin
By virtue of its position, the prim
Phloem Tissue Structurally, phloem
(a) Stalk (b) Culm FIGURE 3-5 Cross
Scale Compressed stem (a) Whole bul
Upper epidermis Palisade layer FIGU
usually occur in xerophytes. In cer
FIGURE 3-22 Selected common leaf ma
FIGURE 3-25 Selected common leaf ti
absorption of water and minerals fr
Outer bark Inner bark FIGURE 3-37 T
Anther Filament Stamen FIGURE 3-41
Exocarp Parts of a typi- FIGURE 3-4
PRACTICAL EXPERIENCE LABORATORY 1.
4.1 CLIMATE, WEATHER, AND HORTICULT
concentration in the atmosphere.A c
TABLE 4-1 Climatic Adaptation of Se
and upward. Another important gener
Rate of photosynthesis mg/sq. dm/hr
and plants that flower under only c
times of the year. Growers start th
content. This section is sometimes
TABLE 4-7 Soil Mineral Nutrients Es
Micronutrients (Trace Elements) Mic
Neutral FIGURE 4-11 A representatio
4.4 FERTILIZERS Fertilizer sources
One of the most commonly used contr
Chlorosis (the yellowing of green l
Fertilizers may be applied before p
It is neither practical nor safe to
Solution: How much of ammonium nitr
1°C (34°F), the optimum temperatu
Cellulose sponge Perched water tabl
Overhead Sprinkler Irrigation Water
FIGURE 4-19 Furrow irrigation of le
can self-install an underground irr
SUMMARY Insects are a major class o
For the home growers or those who c
for consumers and the environment).
TABLE 8-1 Strategy 4: Strategies an
gibberellic acid spray overcomes st
In a competitive industry, a variet
Chemicals gain access to humans thr
2. Pesticide management. Controllin
Every organism has its natural enem
TABLE 8-3 Selected Examples of Biol
1 2 YEAR 3 4 FIGURE 8-5 cycle. A cr
6. Heat treatment. In the greenhous
Organic Compounds (Organics) Organi
under enclosed conditions (e.g., wa
FIGURE 8-9 A tractor-mounted spraye
8.11.9 LANDSCAPE PESTS AND THEIR CO
application, a particular herbicide
Further, they do not provide unifor
SUMMARY Herbicides are chemicals us
Sulfur may be applied for both prev
8.23 PREVENTING GREENHOUSE DISEASES
PART 3 PROPAGATING HORTICULTURAL PL
9 Sexual Propagation PURPOSE AND EX
Anther Microspore Megaspore mother
Lettuce seeds Red light Darkness Fa
FEDERAL AND STATE SEED LAWS Federal
Germination Test In laboratory prac
FIGURE 15 The essential structures
processing into flour or meal). How
physiologically immature seeds must
seeds may be treated in this way be
The two basic modes of seedling eme
locations in the field. Home garden
FIGURE 9-9 A plastic flat. (Source:
(a) (b) FIGURE 9-12 (a) Sowing seed
y the gardener or grower. Whatever
REFERENCES AND SUGGESTED READING Co
species enables vegetative propagat
for rapid rooting. There are two ba
Cutting involving one node (e.g., s
This practice is especially importa
10.6.4 STICKING THE CUTTING Cutting
(a) Indexing by budding Diseased pl
10.11 M ETHODS OF GRAFTING Grafting
Scion Wax FIGURE 10-17 Steps in bar
MODULE 3 BUDDING 10.12 TYPES OF BUD
MODULE 4 LAYERING 10.13 TYPES OF LA
Buried part of shoot is nicked FIGU
variety of ways. In air layering, a
FIGURE 10-34 by using cormels. Prop
Psuedobulbs In the Dendrobium orchi
The technique is used widely in cro
PART 4 GROWING PLANTS INDOORS CHAPT
11 Growing Houseplants PURPOSE AND
TABLE 11-1 Common houseplants Commo
Saddle leaf Philodendron selloum To
Window Displays Plants in windows e
CONTAINER GARDENS DR. TERRI W. STAR
annuals and hardy perennial species
of the large container filled with
perfection about four to six weeks
FIGURE 11-6 Flowers displayed on th
TABLE 11-5 Plant Selected Plants fo
The lighting condition near these w
Fluorescent Lights Fluorescent ligh
may be used for one pot or a group
garden rooms, atriums, or a large c
The photoperiod affects when the ho
patted firm to keep the plant erect
Other Materials Apart from clay and
(a) ( FIGURE 11-25 Support for plan
TABLE 11-7 Common Problems of House
• Keep soil moist all the time
• Prefers high temperatures • P
amount and quality of light. If sup
12 Controlled-Environment Horticult
6. Curvilinear 7. Curved eave 8. Do
Detached greenhouses have several a
12.2.3 FRAME DESIGN There are two b
horticultural business a less-expen
Texas, Hawaii, and California. The
source of heat for times when the t
FIGURE 12-17 Greenhouse production
FIGURE 12-21 Moving tables allowing
Research program on greenhouse engi
greenhouses equipped with a variety
FIGURE 1 Annual energy required per
This system was demonstrated in a 5
FIGURE 6 Amounts of waste energy ut
Ekholt, B.A., D.R. Mears, M.S. Gini
or object to be warmed. Failure to
objects in its path (e.g., the floo
FIGURE 12-27 Motorized ventilation
FIGURE 12-30 Movable internal shade
FIGURE 12-33 A high pressure sodium
Source of Water The quality of loca
FIGURE 12-37 Overhead sprinkler irr
Intermittent Feed Greenhouse plants
However, in winter, greenhouse vent
OUTCOMES ASSESSMENT 1. Explain the
. Foliage or green plants. Foliage
2. Labor. The size of the labor for
FIGURE 13-1 Greenhouse production o
FIGURE 13-2 Lettuce plug is inserte
13.8.4 AGGREGATE HYDROPONIC SYSTEMS
(a) (b) (c) FIGURE 13-6 Plug produc
14 Growing Succulents PURPOSE AND E
FIGURE 14-3 Leaf succulent represen
frost-hardy. Their rosettes are usu
TABLE 14-1 Plant Selected Popular S
(a) (b) FIGURE 14-12 Typical bromel
14.7.1 WHAT ARE CACTI? 14.7 CACTI C
FIGURE 14-16 Opuntia. (Source: Crai
FIGURE 14-23 Mammillaria. (Source:
FIGURE 14-28 Both desert and jungle
Growing mix Gravel Cacti (a) (b) FI
PART5 GROWING PLANTS OUTDOORS: ORNA
15 Principles of Landscaping PURPOS
8. Create recreational grounds. Suc
knowledge, with concern for resourc
(a) (b) (c) FIGURE 15-2 The occurre
GUIDELINES FOR LANDSCAPE DESIGN DAV
the landscape. Some very successful
Rhythm and Line Panoramic view of a
FIGURE 15-10 A formal garden. The e
How frequently do they entertain? A
the patio should be located on the
15.7.1 SELECTING PLANTS A homeowner
Plant Arrangement in the Landscape
Shadow FIGURE 15-15 Planting a tree
SUMMARY Landscaping enhances the su
3. Supply materials on a timely bas
such as preparation rooms (for mixi
of environmental fluctuations. Furt
FIGURE 16-4 A bare-root tree seedli
17 Installation of the Landscape PU
for walks, driveways, and patios (F
Planting may be limited to accentin
17.3.3 PREPARING THE BED The soil s
FIGURE 17-4 Bedding plants raised i
SUMMARY Bedding plants are largely
TABLE 17-6 Selected Ground Covers T
TABLE 17-7 Selected Ornamental Gras
they determine the success and surv
12. Wildlife attraction. Trees in t
pennsylvanica), hackberry (Celtis s
y winds. A stake, which is often a
TABLE 17-8 Selected Narrowleaf Ever
TABLE 17-11 Selected Deciduous Shru
Blooming bushes 1. Blue mist shrub
Planting Bulblets and Bulbils Speci
may be divided such that each secti
FIGURE 18-1 (Source: George Acquaah
Cool-Season (Temperate) Grasses In
Growth Habit Turfgrasses are the mo
Heavy Use Lawns on playgrounds and
The seed should be free from weeds
Source of Sod As with seed, sod sup
A plug of sod FIGURE 18-7 Plugging
way, plants are able to adapt to th
form of a can placed on the lawn wi
TABLE 18-3 Some Common Lawn and Tur
even surface soil surface for layin
MacCaskey, M. 1987. All about lawns
Pruning is sometimes done in conjun
4. Pruning may be done to reduce th
19.4.2 SAWS A saw may be designed t
defeat the purpose of pruning. The
Bud withers as cut end dries back d
19.6 STRATEGIES FOR PRUNING ABOVEGR
Rejuvenation Pruning Cut canes to a
3. In the third and subsequent year
(a) Cut Prune (b) FIGURE 19-16 Step
Eucalyptus and Paulownia are amenab
TRAINING & PRUNING DECIDUOUS FRUIT
Summer pruning eliminates an energy
a) b) FIGURE 2 Newly planted apple
FIGURE 6 Wooden limb spreaders can
FIGURE 9. An apple tree trained to
years to promote continued lateral
Horizontal Espalier The horizontal
19.16.1 CANE FRUITS Cane fruits are
FIGURE 19-26 Shearing of Christmas
pyramid-like form that is wider at
After selecting the appropriate spe
PART 6 GROWING PLANTS OUTDOORS: VEG
20 Growing Vegetables Outdoors PURP
The National Agricultural Statistic
(This item omitted from WebBook edi
growers should take to determine an
pests and reduce/ eliminate hail da
square yard (10 to 68 grams per squ
High tunnels help increase the prof
(This item omitted from WebBook edi
20.4 VEGETABLE MARKET TYPES Fresh V
Establishing the Crop Planting into
home water supply from the tap. Thi
Cole crop Cabbage Root Potato Bean
6. Adequate nutrition. While overfe
variable, ranging from creamy yello
There are two general production pr
This toxin is heat resistant and no
large, or jumbo. The bulb may be sw
REFERENCES Growing selected vegetab
TABLE 21-1 Popular Herbs and Their
(a) (b) (c) (d) (e) (f) FIGURE 21-1
22 Organic Farming PURPOSE AND EXPE
22.3 PRINCIPLES OF ORGANIC FARMING
and the specific materials to be us
22.8 MANAGING SOIL PHYSICAL QUALITY
preemergent or early postemergent o
Composting is a deliberate activity
22.14.5 THE CARBON-TO-NITROGEN RATI
Moisture Supply Water is required b
Compost materials FIGURE 22-4 a wir
As microbial decomposition proceeds
Establishment and Management of an
night, freezing can occur in spring
accomplished by stratification. It
transmitted by the dagger nematode
PART 7 SPECIAL TECHNIQUES AND HANDL
24 Cut Flowers and Floral Design PU
to more than four-fold in standard
Temperature and Humidity Wilting re
FLORAL DESIGN: AN OVERVIEW BY WM. J
Principle Definition Types (or Uses
pH value-a measure of the acidity o
FIGURE 6 Parallel Design-Parallel d
24.3.2 TOOLS AND MATERIALS The tool
3. Establish the focal point. 4. Ad
Natural Drying To dry naturally, fl
24.4.3 DRIED FLOWER ARRANGEMENTS Dr
25 Terrarium Culture PURPOSE AND EX
FIGURE 25-3 Terrarium containers ar
FIGURE 25-5 Assortment of tools use
25.6.7 ENHANCING THE DISPLAY Certai
(a) (b) FIGURE 26-1 Bonsai can be c
TABLE 26-3 Plant A Selection of Pop
26.3.1 COLLECTING BONSAI PLANTS FRO
Strip bark Bare branch FIGURE 26-9
26.5.2 SANITATION It is critical to
27 Postharvest Handling and Marketi
whereas oranges are picked (they ha
(b) (a) (c) (d) (e1) (e2) (f) FIGUR
To reduce packaging injury, contain
is replaced by the by-product of re
Stored produce may lose some color,
with pricing. When selling by volum
(a) (b) FIGURE 27-5 Horticultural p
APPENDIX A Temperature: Converting
APPENDIX B Metric Conversion Chart
APPENDIX D Common and Scientific Na
Pecan (Carya illinoensis) Peony (Pa
GLOSSARY A Abaxial Turned away from
Cellulose A complex carbohydrate th
Floriculture The science and practi
M Macronutrient An essential elemen
Root cap A mass of hard cells cover
INDEX A-frame, 395 A-horizon, 108 A
defined, 390 fertilization, 432-434
Radiant heaters, 378 Radicle, 90 Re
color plate 1 (a) (b) (c) (d) (e) M
color plate 3 (b) (a) (c) (d) (e) (
color plate 5 (a) (b) (d) (c) (e) (
color plate 7 (b) (c) (d) (a) (e) (
color plate 9 (a) (b) (c) (d) (e) (
color plate 11 (a) (c) (b) (d) Grow
color plate 13 (a) (b) (c) (d) (e)
color plate 15 (a) (b) (c) (d) (e)
color plate 17 (a) (b) (c) (d) (e)
color plate 19 (a) (b) (c) (d) (e)
color plate 21 (a) (b) (c) (e) (d)
color plate 23 (c) (b) (a) (d) (e)
color plate 25 (c) (a) (b) (d) (e)
color plate 27 (a1) (a2) (b2) (b1)
color plate 29 (a) (b) (c) (d) (e)
color plate 31 (a) (b) (c) Floral d
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Magazine: Horticulture Principles and Practices