Views
5 years ago

Functional characterization of the SIZ/PIAS-type SUMO E3 ligases ...

Functional characterization of the SIZ/PIAS-type SUMO E3 ligases ...

Functional characterization of the SIZ/PIAS-type SUMO E3 ligases

Plant, Cell and Environment (2010) 33, 1923–1934 doi: 10.1111/j.1365-3040.2010.02195.x Functional characterization of the SIZ/PIAS-type SUMO E3 ligases, OsSIZ1 and OsSIZ2 in ricepce_2195 1923..1934 HYEONG CHEOL PARK 1 *, HUN KIM 1 *, SUNG CHEOL KOO 1 *, HEE JIN PARK 1,3 , MI SUN CHEONG 1 , HYEWON HONG 1 , DONGWON BAEK 1 , WOO SIK CHUNG 1 , DOH HOON KIM 2 , RAY A. BRESSAN 1,3,4 , SANG YEOL LEE 1 , HANS J. BOHNERT 1,5 & DAE-JIN YUN 1 1 Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, Korea, 2 College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea, 3 Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana, 47907-2054, USA, 4 King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia and 5 Departments of Plant Biology and of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA ABSTRACT Sumoylation is a post-translational regulatory process in diverse cellular processes in eukaryotes, involving conjugation/deconjugation of small ubiquitin-like modifier (SUMO) proteins to other proteins thus modifying their function. The PIAS [protein inhibitor of activated signal transducers and activators of transcription (STAT)] and SAP (scaffold attachment factor A/B/acinus/PIAS)/MIZ (SIZ) proteins exhibit SUMO E3-ligase activity that facilitates the conjugation of SUMO proteins to target substrates. Here, we report the isolation and molecular characterization of Oryza sativa SIZ1 (OsSIZ1) and SIZ2 (OsSIZ2), rice homologs of Arabidopsis SIZ1. The rice SIZ proteins are localized to the nucleus and showed sumoylation activities in a tobacco system. Our analysis showed increased amounts of SUMO conjugates associated with environmental stresses such as high and low temperature, NaCl and abscisic acid (ABA) in rice plants. The expression of OsSIZ1 and OsSIZ2 in siz1-2 Arabidopsis plants partially complemented the morphological mutant phenotype and enhanced levels of SUMO conjugates under heat shock conditions. In addition, ABAhypersensitivity of siz1-2 seed germination was partially suppressed by OsSIZ1 and OsSIZ2. The results suggest that rice SIZ1 and SIZ2 are able to functionally complement Arabidopsis SIZ1 in the SUMO conjugation pathway. Their effects on the Arabidopsis mutant suggest a function for these genes related to stress responses and stress adaptation. Key-words: Oryza sativa; SIZ/PIAS-type SUMO E3-ligase; stress response; SUMO; sumoylation. Correspondence: H. C. Park and D.-J. Yun. Fax: +82 55 759 9363; e-mail: hcpark@gnu.ac.kr; djyun@gnu.ac.kr *These authors contributed equally to this work. INTRODUCTION Many eukaryotic proteins are regulated by posttranslational modifications, such as the reversible covalent attachment of ubiquitin (Ub) and ubiquitin-like (Ubl) proteins (Novatchkova et al. 2004; Kerscher, Kerscher & Hoshstrasser 2006). These post-translational modifications critically alter biological activities, subcellular localization and stability of their target proteins (Dohmen 2004). The small ubiquitin-related modifier (SUMO) is highly similar to ubiquitin with respect to its three-dimensional structure. However, SUMO has only about 20% similarity to Ub in its primary sequence and includes approximately 15 additional N-terminal amino acid residues (Hay 2005). SUMO conjugation (Sumoylation) to target proteins involves the sequential actions of activation (E1), conjugation (E2) and ligation (E3) enzymes as in ubiquitination.As a contrasting feature different from ubiquitination, sumoylation is guided by short consensus sequences, Y-K-X-E/D (Y, large hydrophobic amino acid; K, acceptor lysine; X, any amino acid; E/D, glutamate or aspartate) (Schmidt & Müller 2003). Ubiquitination is generally associated with proteasomal degradation of target proteins, whereas sumoylation is linked to roles in transcription regulation, signal transduction in the cell cycle, DNA repair, nuclear import and sub-nuclear compartmentalization (Johnson 2004; Hay 2005).The process is best understood in yeast and animal cells. In the Arabidopsis genome, all components required for sumoylation have been characterized biochemically and genetically (Novatchkova et al. 2004; Colby et al. 2006). These components are essential for plant development and adaptation during abiotic and biotic stresses (Saracco et al. 2007; Miura et al. 2009). Similarly, the sumoylation machinery is also encoded in the rice genome, although studies on the molecular, biochemical and genetic levels are presently lacking (Miura, Jin & Hasegawa 2007a; Suzuki et al. 2009). SUMO E3-ligases confer substrate specificity transferring SUMO from E2 conjugating enzymes to substrates. They are essential components in the sumoylation pathway © 2010 Blackwell Publishing Ltd 1923

Arabidopsis RGLG2, Functioning as a RING E3 Ligase, Interacts ...
Members of the PIAS family act as SUMO ligases for c-Jun and p53 ...
SUMO AND UBIQUITIN LIGASES REGULATE HEPATOCYTE ...
RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for ...
Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses ...
a study on the e3 ligase trim21/ro52
Functional Characterization of OsMADS18, a Member of the AP1 ...
Characterization of the Ubiquitin-Protein Ligase E6-AP by RNA ...
61 Role of the ubiquitin E3 ligase SCFAtSKP2 in cell proliferation 62 ...
Functional Characterization of - Plant and Cell Physiology
Functional Characterization and Expression ... - Plant Physiology
Molecular Identification and Functional Characterization of ...
Functional characterization of TIP60 sumoylation in UV-irradiated ...
MDM2 Acts Downstream of p53 as an E3 Ligase to Promote FOXO ...
Itch E3 ubiquitin ligase regulates large tumor suppressor 1 stability
Physiological functions of the HECT family of ubiquitin ligases
Functional characterization and signal transduction ... - Funpec-RP
Molecular and Functional Characterization of the Arabidopsis ...
Structural and functional characterization of the ... - Microbiology
Functional Characterization of Arabidopsis thaliana WRKY39 in ...
Molecular and functional characterization of ... - URGV - Inra
Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...
Title: A Genome-wide Functional Characterization of Arabidopsis ...
Evolution and Functional Characterization of the ... - Mbio.ncsu.edu
Cloning, functional expression and characterization of a ...
Structural and Functional Characterization on the Interaction of ...
Functional Characterization of Phosphorylation of the Porcine ...
Crystal structure and functional characterization of an ...
Identification and functional characterization of the ... - Glycobiology