Easy IMatrix 511
Highly Efficient ES/iPS Cell Culture Best cell culture substrate from laminin iMatrix series are recombinant human laminin fragment products, optimized as a cell culture substrate (coating material).
Highly Efficient ES/iPS Cell Culture
Best cell culture substrate from laminin
iMatrix series are recombinant human laminin fragment products, optimized as a cell culture substrate (coating material).
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<strong>Easy</strong> iMatrix-<strong>511</strong><br />
Ready to use laminin-<strong>511</strong> fragment : <strong>Easy</strong> iMatrix-<strong>511</strong> series<br />
No more laborious calculation for dilution of coating matrix!<br />
<strong>Easy</strong> iMatrix-<strong>511</strong> / <strong>Easy</strong> iMatrix-<strong>511</strong> silk<br />
iMatrix-<strong>511</strong>, is the recombinant human Laminin-<strong>511</strong> E8 fragment, produced by CHO-S cells or<br />
Transgenic silkworm cocoon.<br />
They can reduce the risk of the xeno-components contamination in the culture.<br />
Compared to regular iMatrix-<strong>511</strong>, <strong>Easy</strong> iMatrix-<strong>511</strong> and <strong>Easy</strong> iMatrix-<strong>511</strong> silk are ready to use<br />
format then do not require dilution step and laborious calculation of concentration.<br />
• Background of Laminin and Laminin fragment<br />
• Features<br />
• Advantage of using Laminin-<strong>511</strong>E8 fragment in ES/iPS cell culture<br />
• Procedure<br />
• Product Information<br />
Background of Laminin and Laminin fragment<br />
Laminin is one of the extra cellular matrix (ECM)s located in the basement membrane of animals, and<br />
play an important role for cell adhesion and proliferation.Laminin is composed of three parts ; alpha,<br />
beta, and gamma - chain and 15 types of laminin is already known. Among them, Laminin composed<br />
by Alpha5-chain, Beta1-chain, and Gamma1-chain, is called "Laminin-<strong>511</strong>".<br />
Laminin-<strong>511</strong> is recognized by alpha6beta1 integrin. Cell adhision, extension and proliferation is<br />
caused by many cell signalings through intergrin.Laminin-<strong>511</strong> E8 is the fragment of Laminin-<strong>511</strong><br />
obtained by enzyme digestion. Although this is fragment, it has binding ability to alpha6beta1<br />
integrin.<br />
PELOBIOTECH GmbH | Order here sales@pelobiotech.com | P.: 0049 (0) 89 517 286 59-0
<strong>Easy</strong> iMatrix-<strong>511</strong><br />
Features<br />
• Ready to use<br />
• Works in various cells - especially good for iPS / ES cells<br />
• Reduce the risk of contamination by xeno-components in your culture system.<br />
• High cell viability and proliferation rate.<br />
Note: This product contains recombinant human serum albumin for stabilization and improvement of<br />
activity.<br />
Advantage of using Laminin-<strong>511</strong>E8 fragment in ES/iPS cell culture<br />
With conventional culture methods, cells die if ES/iPS cells are dispersed into single cells. Therefore,<br />
maintaining cell colony is necessary to a certain extent when passaging.<br />
PELOBIOTECH GmbH | Order here sales@pelobiotech.com | P.: 0049 (0) 89 517 286 59-0
<strong>Easy</strong> iMatrix-<strong>511</strong><br />
Using Laminin-<strong>511</strong> E8 fragment as cell culture substrate enables to maintain and culture even a single<br />
ES/iPS.<br />
- The period of time required for learning cell culture methods is reduced.<br />
- The extension efficiency of cell culture is drastically enhanced.<br />
Procedure<br />
In the case of 6 well culture plate ... (9.6 cm 2 /well)<br />
(1) Add 1.5 mL / well of <strong>Easy</strong> iMatrix-<strong>511</strong> to the plate.<br />
(2) Incubate the plate at 37℃ for a hour at RT or 4℃ for overnight.<br />
(3) After (2), discard solution and start culture shortly. (DO NOT DRY!)<br />
Caution : For iPS cells, recommended cell density is 2 - 3 x 10^3 cells/cm 2 .<br />
However, culture condition must be optimized.<br />
Click here for the extended culture protocol for hES/hiPS cells using iMatrix-<strong>511</strong>. (External site.)<br />
Click here for the video for the passage procedure for hES/hiPS cells using iMatrix-<strong>511</strong>. (External site.)<br />
See the product information of regular iMatrix-<strong>511</strong> from here.<br />
Download Datasheet here: <strong>Easy</strong> iMatrix-<strong>511</strong>/<strong>Easy</strong> iMatrix-<strong>511</strong> silk<br />
Distributed by PELOBiotech GmbH<br />
Am Klopferspitz 19<br />
82152 Planegg<br />
Germany<br />
P. 0049 (0) 89 517 286 59 0<br />
Email: sales@pelobiotech.com<br />
PELOBIOTECH GmbH | Order here sales@pelobiotech.com | P.: 0049 (0) 89 517 286 59-0
<strong>Easy</strong> iMatrix-<strong>511</strong><br />
PELOBIOTECH GmbH | Order here sales@pelobiotech.com | P.: 0049 (0) 89 517 286 59-0
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