PELONEWS WITH FOCUS ON CELLS & MEDIA
PELOBiotech Newsletter Nov/Dec. 2018
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Relevance of Kidney Organoids
Kidney organoids are relevant from several perspectives, including modelling of kidney disease and injury. Commonly-used
in vitro two-dimensional cell (2D) models are limited because cells behave differently in a 2D configuration
and cannot constitute in vivo-relevant cell-cell interactions. Besides, contemporary in vivo animal models
often fail to represent human diseases due to species-species differences. Modelling diseases using organoids
surpasses these problems. Stem cells from patients with a certain disease can be grown to organoids, which can
experimentally be manipulated in each disease context. Gut organoids are currently the best-established organoid
disease model and are already used in practice to model cystic fibrosis. In most cystic fibrosis patients, a dysfunctional
CFTR gene causes improper fluid
transport along the gut epithelium, which expresses
in abnormally thick and sticky
mucus. CFTR is therefore important in fluid
transport in the gut, which could be
observed by patient gut organoids swelling
less than control gut organoids, an
observation in parallel with the disease phenotype.
Kidney organoids have also
been shown to be relevant in modelling acute
kidney disease, because in vivo-
relevant biomarkers of kidney and DNA damage
show expression in organoids after
injury infliction. It has been shown that, after
treatment of kidney organoids with
antibiotic gentamicin or anti-cancer drug cisplatin,
organoid cells showed expressi- on of kidney injury marker 1.
Organoids can also be used to screen
for efficacy and safety of potentially diseasecuring
drugs. Drug development cur-
rently experiences that 66% and 30% of all
preclinically validated drugs fail to en-
ter the next developmental stage from phase
II and phase III, respectively. This
phenomenon illustrates the huge gap between
preclinical drug validation and success
of these drugs in the clinic, which exists because
the simple two-dimensional in
vitro cell models often do not resemble the
much more complex in vivo patholo-
gies. Large-scale use of organoid systems
in drug screening might bridge the gap
between pre-clinical validation and use of the
drug in the clinic, as they are consi-
dered to more closely resemble the in vivo
situation than two-dimensional cell
cultures.[11, 7] Drug screens employing organoid
systems have already proven
successful. For example, Huang et al. performed
screening of candidate drugs to treat pancreatic cancer on organoids derived from patient stem cells, and
found that the organoids responded better than other candidate drugs to UNC1999, an inhibitor of epigenetic regulator
H3K27me3. Also from the perspective of safety, kidney organoids would be a useful tool in drug nephrotoxicity
screenings. Compared to all other cell types, kidney cells are especially sensitive to toxins, xenobiotics, metabolic
waste products and other potentially harmful compounds,[5, 9] mainly because these compounds become
highly concentrated in renal cells due to extensive water resorption. Nephrotoxicity in kidney cells and subsequent
kidney dysfunction is therefore a common complication of drug administration.[9, 10]
Noviocell BV is a highly
in Oss, The
Netherlands. It focuses
on the development of
the first ever synthetic
solution for 3D cell culturing,
organoid growth to
Astashkina, A. and D.W. Grainger, Critical analysis of 3-D organoid in vitro cell culture models for
high-throughput drug candidate toxicity assessments. Adv Drug Deliv Rev, 2014. 69-70: p. 1-18.
Ranga, A., N. Gjorevski, and M.P. Lutolf, Drug discovery through stem cell-based organoid models.
Adv Drug Deliv Rev, 2014. 69-70: p. 19-28.
Gardner, J., What you need to know about cystic fibrosis. Nursing, 2007. 37(7): p. 52-5.
Dekkers, J.F., et al., A functional CFTR assay using primary cystic fibrosis intestinal organoids. Nat
Med, 2013. 19(7): p. 939-45.
Morizane, R., et al., Nephron organoids derived from human pluripotent stem cells model kidney
development and injury. Nat Biotech, 2015. 33(11): p. 1193-1200.
Au, S.H., et al., Hepatic organoids for microfluidic drug screening. Lab Chip, 2014. 14(17): p. 3290-9.
Wang, S., D. Gao, and Y. Chen, The potential of organoids in urological cancer research. Nat Rev
Urol, 2017. 14(7): p. 401-414.
Huang, L., et al., Ductal pancreatic cancer modeling and drug screening using human pluripotent
stem cell- and patient-derived tumor organoids. Nat Med, 2015. 21(11): p. 1364-71.
Fisel, P., et al., Solute carrier transporter and drug-related nephrotoxicity: the impact of proximal
tubule cell models for preclinical research. Expert Opin Drug Metab Toxicol, 2014. 10(3): p. 395-408.
Kandasamy, K., et al., Prediction of drug-induced nephrotoxicity and injury mechanisms with human
induced pluripotent stem cell-derived cells and machine learning methods. 2015. 5: p. 12337.
Xinaris, C., V. Brizi, and G. Remuzzi, Organoid Models and Applications in Biomedical Research.
Nephron, 2015. 130(3): p. 191-9.
Chuah, J.K. and D. Zink, Stem cell-derived kidney cells and organoids: Recent breakthroughs and
emerging applications. Biotechnol Adv, 2017. 35(2): p. 150-167.
Complete ECM Surface Coating Kit
We are pleased to announce the launch of the NativeCoat product line to bring the highly specific
biochemical composition of native cell microenvironment to an in vitro setting.
Coating with cell culture surfaces with NativeCoat results in:
Improved cell attachment
Increased cell viability and proliferation
Activation of signaling pathways
If you like to know more,
meet East River in November
in Munich – and
enjoy the great view
from the Sky Lounge.
Tickets are limited, to reserve your
spot please sign up via email
or give her a call:
+49 89 517 286 59-0
3D Micro-Tumour System
abc biopply is introducing a fully standardised 3D micro-tumour system based on its 3D
CoSeedis technology platform. The new 3D CoSeedis Micro-Tumour System –
WiDr offers fully validated protocols and includes all required components to successfully
grow tumour cells into 3D spheroids. It is ideally suited to establish the 3D CoSeedis
technology in your lab and provides easy-to-follow SOPs for:
3D aggregate formation
Maintenance of aggregates for up to 10 weeks
Harvest of grown spheroids
The system allows to monitor tumour growth in real-time and to accurately measure
growth-related effects of treatments.
Furthermore, the system is fully compatible with abc biopply’s organoid histology system.
Adapting the system to other cell types than WiDr can easily be achieved and abc biopply
also offers a customised service to assist.
Contact us for further details and information.
Pic : abc biopply
Try New Gold
Bacteria, yeast, zebrafish,
mice – for decades,
these model organisms
have supported the research
to facilitate breakthrough
human health. But the
gold standard – a human
cell model system – has
been difficult to set-up,
handicapped by genetic
modifications, and prohibitively
Through unique antibody
-free cell isolation methods
by our partners at
Cell Systems, primary
human cells can now be
easily incorporated into
your research program
for more relevant and
accurate insights and
validation. Their deep
inventory allows a consistent
over large-scale studies,
and can be customized
as large lots to supply
the pharmaceutical industry.
Ask your research questions
using the new gold
standard tool: antibodyfree
our partner Cell Systems.
Check out our NEW Products
Anti-SOD1 (ALS-related mutants) Clone : MS785, MS27
New powerful tools to research pathophysiological role of SOD1 mutants in ALS patients
Amyotrophic Lateral Sclerosis (ALS) is a fatal adult-onset neurodegenerative disease. According to
many research results, SOD1 (Cu/Zn superoxide dismutase) is one of the major causative genes of ALS
and SOD1 mutant proteins cause (evoke, lead) ALS through a gain of toxic function.
Wild type SOD1 forms homo dimer. However, it was reported that mutant SOD1 forms the different conformation,
having toxic function by binding to Derlin-1, an ER-associated degradation (ERAD) machinery
protein. Prof. Hidenori Ichijo
and his colleagues developed
two novel rat monoclonal
clone MS785 and MS27,
which specifically bind to
SOD1, not detecting wild
type SOD1 homo-dimer.
Both clones are succeeded
in detecting over 100 SOD1
mutants (Ref.2), please visit
our website for further information
about the list of detectable
Specific to SOD1 mutants
Application ; IP and IC : Detect SOD1 mutant ELISA : Detect SOD1 mutant Western Blot : Detect SOD1 wild
and mutant type.
Note In denatured condition, MS785 and MS27 detect both wild and mutant type of SOD1
Read more and download your flyer.
Ask for our extensive product list of Antibodies: firstname.lastname@example.org
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