WITH FOCUS ON IPS/ES CELLS
PELOBiotech Newsletter Sept/Oct. 2019
„Actually, science begins
to become interesting only
where it ends.“
-Justus von Liebig, German
-> Endothelial Transwell Permeability
-> ATAC-Seq Kit
-> HemEx-TypeA Medium
-> LIFOR— Cryo/Preservation
Medium ORDER SAMPLEs
As every year we can´t wait for the Wiesn—with lots of fun, centrifugal
forces and good measure of ice cold preservation in liquid
Mass, Visit now www.pelobiotech.com and check out our
Offers. Before you enjoy your beer— we have a question for you:
What‘s correct: O´Zapft is or I`‘zapft os! ??? And who said the
misspelled version and when ? Do you know? Cheers!
Editor in Chief PELONews
IPSC-Medium | iPSCS xenofree
Medium | Xyltech | Laminin
511 | IPSelector
Cell Culture Media
PeloNews: Meet us at TEDD,
The French Connection: New Faces & Sales Contacts
New Service for our great Frenchspeaking customers: We like to welcome
Dr. Muriel Malaisé as our inhouse technical rep for France at PELOBiotech.
I also like to introduce to you Michel Vidal and Gilles Moraldi. They are
representing our products and services to you in the North and South of
France and very happy to talk to you about your research projects and challenges.
They are both very experienced in the Life Science sector and can
support you in your daily business. Please contact us or your personal
contact in France today.
Nord: Michel Vidal : Mobile + 33 (0)6 07 75 16 53 | Hauts de France, Ile de
France, Normandie, Bretagne, Val de Loire, Centre, Grand Est, Bourgogne
South: Gilles Moraldi | Mobile +33(0)6 16 54 52 45 | Auvergne Rhône-
Alpes, Aquitaine, Midi-Pyrennées, Occitanie, Provence Alpes-Côte d’Azur
New Kits on the Block:
Endothelial Transwell Permeability Assay Kit
Our partner Cell Biologics has developed a simple, reproducible, sensitive,
quantitative transwell permeability assay to measure endothelial
barrier function for wide analyses of multiple types of endothelial permeability in vitro. The assay will
be performed by a spectrometer-based absorbance reader (ELISA plate reader) at 450 nm within one
hour post endothelial treatment. The assay kit is suitable for primary endothelial cells and immortal cell
lines from multiple species.
The microvascular endothelial cell monolayer localized at the critical
interface between the blood and vessel wall has the vital functions of
regulating tissue fluid balance and supplying the essential nutrients
needed for the survival of the organism. The endothelial cells are able
to dynamically regulate its paracellular and transcellular pathways for
transport of plasma proteins, solutes, and liquid.
Please click here: https://
The endothelial barrier is a well-regulated structure, which maintains
a minimal and selective permeability to fluid and molecules under normal
physiological conditions. Vascular leakage is an important feature
in several diseases, such as septic shock, inflammation, disruption of
blood-brain barrier (BBB)-related neurological disorders, viral hemorrhagic fever, cancer and ischemiareperfusion
injuries. It is emergent to develop a highly sensitive and reliable assay to measure endothelial
The ATAC-seq kit is a complete set of optimized reagents that are ideal to generate indexed DNA libraries
for ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing).
The Kit includes Tn5 Transposase and tagmentation reagents, and also library construction reagents
(index-primers, enzyme et al), with this kit you can obtain qualified ATAC-Seq library DNA ready to perform
sequencing on Illumina sequencer.
Low input, requires sample as low as 50,000 live cells
Fast workflow, with minimal hands-on time
Please click here: https://
Robust, reliable performance
Ultra high-fidelity amplification with minimized GC bias
Application: Prepare ATAC-seq DNA library for Illumina sequencer
World's First Medium for Mouse (HSCs)
Hematopoietic Stem Cells for Maintaining
HemEx-TypeA is a serum-free media, which contains Polyvinyl Alcohol
(PVA) and can be used for mouse Hematopoietic Stem Cells
Optimized for the
mouse HSCs, Hematopoietic
Cell (HSPCs) obtained
Can do expansion
culture of HSCs,
for more than 1
month in undifferentiated
PVA is used
instead of albumin.
In general, culture media for HSCs contains serum albumin. Such albumin
is required to induce cell division of HSCs. On theother
hand, albumin inhibits stable undifferentiated state.
Widely used purified or recombinant albumin contains small amount of
contaminants,but are tough to remove.
It is reported that such contaminants induce differentiation of HSCs.
Moreover, protein's oxidation reaction inducescellular senescence.
Thefore, albumin needs to be replaced by other chemicals.
Dr. Satoshi Yamazaki at The Institute of Medical Science, The University
of Tokyo reported that PVA can replace albumin (1).
Moreover, PVA is a stable chemical with no oxidation reaction in medium
so aging of HSCs are also suppressed. These advantages help to
do expansion culture of HSCs while maintaining undifferentiated state.
Please see the paper
of Nature, 571, 117 -
Note : For culture,
addition of cytokine
(1) Adam C., et al. Nature, 571, 117 - 121 (2019).
Long-term ex vivo hematopoietic stem cell expansion allows nonconditioned transplantation
(paper is attached).
Preservation- and Cryo-Medium
No need to flush or
No animal serum
No animal protein
No need for DMSO
Storage at RT for up to
Storage at 4-8°C for up
to 7 days
LIFOR TM —Preservation-Medium
Great For Biopsy:!! LIFOR next generation solution preservation
medium significantly extends the preservation time of tissue, cells, organs
and biopsies. We use a proprietary liposome-based technology
that acts at the cellular level, preventing tissue and cell damage over a
wide range of temperature gradients, including room and refrigerated
temperatures. Continually nourishes samples with oxygen and nutrient
Note: No need for DMSO
LIFOR TM —Cryo-Medium
LIFOR is a novel chemically defined medium combined with nanoparticles,
dextran and nutrients. LIFORnext generation cryopreservation
medium significantly improves cell viability. We use a proprietary liposome-based
technology that acts at the cellular level, preventing cell damage
caused by ice crystallization. LIFOR is an Extra-Cellular type
solution that mimics the extra-cellular environment of cells. LIFOR
does not contain DMSO giving you the flexibility to add desired quantity
and thereby controlling outcomes.
You add the amount of DMSO as needed: For human primary cells
(HUVEC) 3 % DMSO we`ll enough to have great results.
Have a look at this interesting paper:
Learn more here
ORDER YOUR SAMPLE HERE
Please order your sample now, ask Muriel, Peter and Lothar:
Just send an email to mailto firstname.lastname@example.org
Breakthrough: High Performance Medium
for iPSC ReproMed iPSC Medium
From the pioneer of stem cell culture media in Japan, Reprocell, comes a breakthrough
formulation for Regenerative Medicine:
Manufactured under GMP compliance
Suitable for clinical research applications
ReproMed iPSC Medium is formulated and designed for
cultivation of iPS cells destined for cell therapy applications.
In close consultation with regulatory authorities we have formulated
a medium with components meeting the strict purity
and traceability standards of Japan. ReproMed iPSC Medium
is validated for tri-lineage pluripotency by in vitro differentiation
into mesoderm, endoderm and ectoderm cell lineages.
Please click : https://
NEW: Introducing High Performance
iPSC Xeno-Free Growth Media
Xeno-free and chemically-defined medium devoid of animal- or human-derived
components. For stem cell expansion or other uses that must avoid variability
due to undefined animal components. Choose when media must be entirely
free of animal-derived components, serum & albumin, replaced with recombinant
or synthetic growth factors, amino acids, vitamins, lipids, insulin, etc.
Why Choose Cell Applications Xeno-Free Media?
Cell growth otimized
Prepared in cGMP facility
QC tested & validated
Please click : https://
Xyltech: Human Pluripotent Stem (ES/iPS)
Cells Proliferation Control Medium
Take the weekend off! 72hr change-free.
BOF-01 is a novel basal culture medium which can suppress proliferation
of human ES/iPS cells on feeder cell layers.
BOF-01 can be used by completely replacing the basal culture medium
(e.g. DMEM/F12) of the human ES/iPS cells.
Human ES/iPS cells can be maintained with BOF-01 for about 3days (up to 72
hours) without changing medium under normal culture conditions (37°C, 5%
CO2).After suppression of cell proliferation with BOF-01, cell growth can be
resumed by changing normal growth medium.
Please click: https://
Easy to use
Works in various
cells - especially good
for iPS / ES cells
Reduce the risk of
contamination by xenocomponents
in your culture
High cell viability and
Ready to use laminin-511
fragment: Easy iMatrix-511
No more laborious calculation for dilution
of coating matrix!
iMatrix-511, is the recombinant human Laminin-511 E8 fragment, produced
by CHO-S cells or Transgenic silkworm cocoon.
They can reduce the risk of the xeno-components contamination in the
Please click here: https://
Promotes greater stem cell adhesion than all other matrix proteins
that have been tested
E8 fragments retain integrin binding specificity and capacity, and display
higher potency than natural Laminin-511
Background: Laminin is one of the extra cellular matrix (ECM)s located
in the basement membrane of animals, and play an important role
for cell adhesion and proliferation.Laminin is composed of three parts ;
alpha, beta, and gamma - chain and 15 types of laminin is already
known. Among them, Laminin composed by Alpha5-chain, Beta1-chain,
and Gamma1-chain, is called "Laminin-511".
Specific for undifferentiated
human iPS or
Uniformly stain cell
membrane of human
iPS or ES cells.
Can be used for removing
human iPS or ES cells
by cytotoxic effect.
Clone : R-17F
Please click here
iPS/ES cells Specific Monoclonal
Antibody : iPSelector
iPSelector is a mouse monoclonal antibody recognizes
for lacto-N-fucopentaose I (LNFP I) raised
against human iPS cells. LNFP I is the sugar chain
expressed in undifferentiate state human iPS or ES
cells then this antibody can be used as a marker for
undiffrentiated human iPS or ES cells.
Moreover, this antibody has a function for cytotoxic
effect by binding to undifferentiated human iPS or ES
cells. Therefore this antibody is beneficial for removing
undifferentiated human iPS or ES cells.
1.Kawabe, K., et al., Glycobiology, 23, 322, (2013).
2. Matsumoto, S., et al., J. Biol. Chem., 290, 20071, (2015).
Nakao, H., et al., Glycoconj. J., DOI 10.1007/s10719-0169-9710-2
Pic: Western blot
One major positive band
and several minor bands
were specific to human iPS
cells, and any positive
band were not obtained
with HEK293 cells.
Take it personal! New Customized Media for
Animal & Human cell culture
You have your very specific cell culture needs
in your lab. Because you are unique and your
research is so too.In this webinar with CSO Dr.
Lothar Steeb, PELOBiotech, you´ll get an
overview of our services to customize your
academic or industrial research and production
Biomedical research is increasingly focusing
on genetic profiles, marker invention and other
patterns that characterise cells and processes.
More and more, gene analytical investigations
are moving into the focus of experiments. Exosomes,
miRNAs, smallRNAs and epigenetics
play an increasingly important role.
We contribute to this development by manufacturing special media
which are coordinated with the customer on a trial and cell basis.
We offer the customer every modification of classical media as well as
new chemically and thus serum-free media variations, which he
needs in order to exclude disturbing factors from the outset and thus
avoid false positive or false negative results.
Examples are RPMIs, DMEMs, SILAC BIT, chemically defined renal epithelial
cell media or all types of glucose-free media.
Sign up and click here: https://attendee.gotowebinar.com/
register/2810266360323725836 or https://www.pelobiotech.com/peloacademy/
NEW Ways: Grow Cells Quicker with UltraGro
UltraGRO-PURE meets your need for a consistent and reliable human
protein based cell culture supplement that can support
your cell production from research through to clinical
trial use. UltraGRO-PURE contains abundant
growth factors and cytokines necessary for cell growth
and proliferation to replace fetal bovine serum (FBS)
for growing Adipose-Derived (AD), Bone Marrow (BM)
or Umbilical Cord (UC) Mesenchymal stem cells
Growth kinetics for MSCs expanded in 5%
UltraGRO-PURE containing medium are comparable to cultures grown in
medium supplemented with 20% FBS. UltraGRO-PURE grows cells quicker
and safer, lowering your costs per volume of cells grown.
More infos here: https://www.yumpu.com/s/cvIPngFTbGiR0lK6
Ask Peter, Lothar and Muriel for your Personal Sample:
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lIVE events? No worries.
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send you the recording
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and informed? Then
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October 10, 2019
10 AM (CET/MEZ)
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friends. Thank you.
-> Cost-effective, nonxenogeneic
->Natural source and
natural signal for cell
-> Abundant growth
factors / cytokines / proteins
-> Lot to lot consistency
Extended storage for
without losing potency
-> Research grade and
GMP grade available
-> Cost-effective, nonxenogeneic
PELONews: Meet us at...
TEDD Save the date for the
next TEDD Meeting: October
24, 2019 in Wädenswil, Switzerland.
The Tisssue Engineering for
Drug Development and Substance
Testing (TEDD ) Annual
Meeting with the title Cell
Sources and Stem Cell Generation
for Drug Development
brings together experts from diverse
fields with a shared interest
in advanced 3D models.
Join us at this meeting to celebrate
another fruitful collaboration year with the new perspectives
Talk with Dr. Peter Frost (PELOBiotech, see
pic right) and Marco Leu (abcbiopply, left)
about Cell Sourcing and the cool
3D Co Culture System 3D CoSeedis.
Please click here or https://
Got the right answer?
Erich Kiesl caused the
biggest mishap so far
in the Octoberfest history:
In the eagerness
of the battle he forgot
1978 the obligatory
The next time he
tapped, he managed
to confuse the vowels
and shouted loudly
Some people swear
they have heard:
GfMVB Also meet our CSO Dr. Lothar Steeb at the GfMVB , German
Society for Microcirculation and Vascular Biology in Heidelberg.
October 25 to 27, 2019
Topics will be
Organ-specific vascular features
Endothelial cell-cardiomyocyte communication
Biomechanical control of vascular differentiation &
Microcirculatory tumor progression and metastasis
Vascular homeostasis, aging & repair
Immuno-vascular communication skills
Please click here or https://
You like what you see? Sign up for PELONews
now at www.pelobiotech.com or just fill out the
following form online.
If you need any further
assistance or if you
like what you see, tell
82152 Planegg |
+49 89 517 286 59 0
Dr. Peter Frost,
Dr. Lothar Steeb
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