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Phylogenetic analysis of West Nile Virus in Maricopa County,

Arizona: Evidence for dynamic behavior of strains in two

major lineages in the American Southwest

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Phylogenetic analysis of West Nile Virus in Maricopa County,

Arizona: Evidence for dynamic behavior of strains in two

major lineages in the American Southwest

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File Size: 3451 KB Print Length: 23 pages Simultaneous Device Usage: Unlimited Publication Date: March

20, 2020 Sold by: Amazon.com Services LLC Language: English ASIN: B0867MZP9J Text-to-Speech:

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Book Description

West Nile Virus (WNV) has been detected annually in Maricopa County, Arizona, since 2003.

With this in mind, we sought to determine if contemporary strains are endemic to the county or

are annually imported. As part of this effort, we developed a new protocol for tiled amplicon

sequencing of WNV to efficiently attain greater than 99% coverage of 14 WNV genomes

collected directly from positive mosquito pools distributed throughout Maricopa County between

2014 and 2017. Bayesian phylogenetic analyses revealed that contemporary genomes fall within

two major lineages; NA/WN02 and SW/WN03. We found that all of the Arizona strains

possessed an amino acid substitution known to be under positive selection, which has arisen

independently at least four times in Arizona. The SW/WN03 strains exhibited transient behavior,

with at least 10 separate introductions into Arizona when considering both historical and

contemporary strains. However, NA/WN02 strains are geographically differentiated and appear to

be endemic in Arizona, with two clades that have been circulating for four and seven years. This

establishment in Maricopa County provides the first evidence of local overwintering by a WNV

strain over the course of several years in Arizona. Within a national context, the placement of

eleven contemporary Arizona strains in the NA/WN02 lineage indicates while WNV first entered

the northeastern United States in 1999, the most ancestral extant strains of WNV are now

circulating in the American southwest.


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