PELONews Summer 2020
Read the latest edition of PELONews with PELOAcademy some topics are GMP, BBB, Dyes &Cell Imaging Reagents, Covid-19, 3D Cell Culture, cancer
Read the latest edition of PELONews with PELOAcademy
some topics are GMP, BBB, Dyes &Cell Imaging Reagents, Covid-19, 3D Cell Culture, cancer
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PELONEWS<br />
WITH FOCUS ON WELCOME TO LAB<br />
PELOBiotech Newsletter <strong>Summer</strong> <strong>2020</strong><br />
"Everything will be<br />
okay in the end.<br />
If it´s not okay it´s not<br />
the end.“<br />
- John Lennon - English singer<br />
& songwriter -<br />
Pic: Pixabay<br />
Dear Scientist,<br />
Yes, you can´t wait to get back to my bench, lab&office. Me too. So welcome<br />
everybody to óur new reality. We know that COVID-19 has impacted<br />
upon your research, so we like to help you get back on track as fast<br />
as possible. We are ready to support you in any kind you need.<br />
And we are as fast as always an d already updates our prices with the<br />
reduced tax rate in Germany. So no worries about that...<br />
If you would like to order your products now and hold delivery until you<br />
are able to return to the lab, please let us know upon ordering. We will<br />
be happy to assist you in smoothing the transition back to the bench<br />
whenever you are ready and deliver when you need us. Please check<br />
with your facility whether now there are changes in your logistics routine.<br />
Are you still accepting and getting deliveries all the times? We will<br />
check in with you before we send out your delivery as we ask you politely<br />
to do the same. Thank you.<br />
To discuss this our logistics, customer success&support are here for any<br />
of your questions. Online resources are available to teams around the<br />
world, so you can schedule an Online-Meeting and Support anytime via<br />
phone or Go-to-meeting. We are just one click away.<br />
On behalf of the global PELOBiotechTeam, we’re here for you.<br />
Take very good care and stay safe!<br />
Christiane Büchsel<br />
Editor in Chief <strong>PELONews</strong><br />
PS Also watch out for our online-attendence at GSCN - the German<br />
StemmCell Network. We love to meet you there.<br />
Content<br />
Products related to CO-<br />
VID-19 Research & Dyes<br />
PELOAcademy:<br />
Join us every week for<br />
new refreshing webinars<br />
and our new Series:<br />
Can Cell Culture be as<br />
good as in vivo today?<br />
2D/3D Cell culture: July 23<br />
NEW Products<br />
Watch a fresh Webinar:<br />
Dyes&Cell Imaging Reagents<br />
Our Portfolio<br />
See what we got
<strong>PELONews</strong><br />
<strong>PELONews</strong>: Cancer Research&COVID-19 List<br />
NEW Unique<br />
Imaging Reagents<br />
• Low toxic nucleus imaging<br />
dye = NucleoSeeing<br />
• Washable fluorescent dye<br />
for cytoplasm = CytoSeeing<br />
• Long term imaging fluorescent<br />
dye for ER = ERseeing<br />
• Highly sensitive fluorescent<br />
dye for Lipid<br />
Droplets = LipiDye<br />
• Fatty acid degradation<br />
(FAO) imaging reagent =<br />
FAOBlue<br />
• Intracellular temperature<br />
imaging dye = Thermoprobes<br />
NEW Overview Dyes & Cell Imaging Reagents<br />
As our customer, you can find<br />
all commercially available<br />
primary cells in our product<br />
range.<br />
PELOBIOTECH is your choice<br />
to fulfill all needs you actually<br />
have or that will come up<br />
during your project. PELOBIO-<br />
TECH combines long lasting<br />
expertise with high quality<br />
production methods in cells<br />
and media to build up a solid<br />
basis for your work. Be surprised<br />
what now is state of the art<br />
and clears your path to success.<br />
To get more infos please<br />
send an email to<br />
cellcultureexpert@pelobiotech.com<br />
subjectline<br />
„cell Imaging“.<br />
COVID-19 Research Products&Tools<br />
https://www.yumpu.com/en/document/read/63207661/human-primary-lung-cellslist-<strong>2020</strong><br />
MORE<br />
LUNG cells (Ace2 is the entry point of the Coronavirus in lung epithelial<br />
cells). Most primary Cells from Healty donors and diseased cells Smokers,<br />
Hypertension, Diabetes type 2, Asthma, COPD and more. Build your own<br />
assay with all these risk grouped . Also use our Cell Sourcing. Anti-SARS-2<br />
drug delvelopment.<br />
<br />
Lung endothelial cells microvasucular<br />
Download our special<br />
Covid19-product list<br />
here<br />
https://www.yumpu.com/<br />
en/embed/view/<br />
uEUNB4wVar3FCQ5n<br />
<br />
<br />
<br />
<br />
Lung fibroblasts<br />
Alveolar epithelial cells<br />
Tracheale bronchial smooth muscle cells<br />
Lung epithelial cells and more. See also page 6 for more new products.
PELOAcademy: Our Webinar-Series<br />
PELOAcademy<br />
3D Cell Culture-Series: 2D/3D Cell Culture<br />
presented by Dr. Peter Frost, CEO/CSO, PELOBiotech<br />
Thur July 23, 0220 10 AM German &3:30 PM (CET) English<br />
In the last 10 years Dr. Peter Frost was involved in 3D Cell Culture doing<br />
mainly business development for techniques like liquid overlay, magnetic<br />
spheroid formation, hanging drop technology and others. In the<br />
beginning it was a challenge to convince scientist to use 3D Cell Culture<br />
for their research and devlopments. 3D Cell Culture is nowadays more<br />
or less established in basic research, drug development, compound testing<br />
and personalized cancer diagnostics.<br />
PELOAcademy continues<br />
its series of Webinars:<br />
Can Cell Culture be as<br />
good as in vivo<br />
today? Roadmap to a<br />
better Worfkflow in<br />
your lab.<br />
We will discuss with you the<br />
most important steps during<br />
your workflow each 4th<br />
Thursday of the month in<br />
Today it is more the question and challenge to pick out the best possible<br />
3D cell culture technology for a scientists application or for your read<br />
out. This webinar will summarize many of the excisting techniques in<br />
context to different applications and you will get:<br />
- >Overview of excisting 3D techniques to perform 3D cell culture<br />
- >Advantages/Disadvantages of diferent techniques in context to some<br />
application<br />
-> Possible read out systems for 3D spheroids<br />
NEW Products - Unique Imaging<br />
Reagents and Advanced Cell Culture Tools<br />
Watch recording and get your overview presented by Funakoshi, Japan Join<br />
here: www.pelobiotech.com/pelo-academy<br />
German and English.<br />
No hassle. Watch your<br />
recording on demand.<br />
Part 1- The Informed<br />
Consent Part 2. Tissue<br />
Isolation & Cell Viability<br />
Part 3 Cell Seeding &<br />
Cell Requirements Part 4.<br />
Serum-&Xeno-free Cell<br />
Culture Media Part 5.<br />
2D&3D Cell Culture<br />
Coming soon:<br />
Tissue & Cell Sourcing,
<strong>PELONews</strong><br />
New Products & New Partners<br />
#3DCellCulture<br />
#HTS<br />
#spheroids<br />
TAGS<br />
3D CoSeedis comprises a<br />
family of chips suited for various<br />
amounts of 3D constructs.<br />
They range from 200 spheroids<br />
(3D CoSeedis Chip200) to<br />
880 spheroids per chip (3D<br />
CoSeedis Chip880), both in 6<br />
-well plate format, and also<br />
include a version for 680<br />
spheroids per chip (3D<br />
CoSeedis Chip680) for 24-<br />
well plates. Chips can either<br />
be purchased in single packages<br />
or in bulk.<br />
Download your copy of the<br />
paper here<br />
and contact us for further<br />
questions and information.<br />
NEW White paper by abc bioplly: How to<br />
Revolutionise the Use of 3D Cultures in HTS<br />
Our partner abc biopply has released a white paper demonstrating the unprecedented<br />
quality of 3D CoSeedis in the production of highly homogenous<br />
and uniform spheroids for the use in high-throughput and highcontent<br />
screening applications.<br />
The paper was done in collaboration with Union Biometrica, Inc. (USA) and<br />
shows how 3D CoSeedis can be used as a manufacturing tool to produce<br />
large quantities of homogenous and uniform 3D spheroids in a HTS/HCScompatible<br />
384-well format.<br />
The 3D CoSeedis<br />
platform thereby not only<br />
sets new standards in highvolume<br />
production of 3D<br />
spheroids but it also helps<br />
to substantially increase<br />
screening efficiency. It’s<br />
based on a 24-well plate<br />
format for aggregation,<br />
growth and harvesting and<br />
the resulting spheroid suspension<br />
is used to seed<br />
384-well plates with individual<br />
spheroids using Union Biometrica’s large particle sorter. The resulting<br />
384-well plates contain viable and uniform spheroids in more than 98% of<br />
all the wells and show minimal size variability from one plate to another.<br />
3D CoSeedis thereby helps to decrease screening failure rates and<br />
consequently increases overall screening efficiency<br />
NEW Media & New Partner for MSCs<br />
Cell culture is a critical component of research, development and production<br />
in the life sciences. Cell culture media in particular, has a significant impact on<br />
the efficacy and sensitivity control of the cell culture microenvironment.<br />
PELOBiotech is strenghtening its leading position in the field of MSCs & GMP:<br />
We added two media for MSCs to our portfolio called CellCor, a serum free<br />
chemically defined media composed of over 200 known compounds.<br />
Through 10 years of tenacious research, producer Xcell Therapeutics , South<br />
Korea, developed CellCor SFD MSC and CellCor CD MSC products, which<br />
provide superior performance with the upmost focus safety and quality, assured<br />
by strict adherence to regulated standards following good manufacturing<br />
practices. CellCor does not contain any animal nor plant derived components,<br />
they are hPL free, and Exosome free, available in R&D and GMP<br />
quality.
New Products<br />
Myell: Get clear pictures of Live Cell Imaging<br />
Myell series is a polymer "superthin" film "which is a novel alternative<br />
tool for cover glass" and offers a new method to make sample for microscope<br />
observation.<br />
By using with Myell series, the samples are prevented from vibrating. This<br />
makes us to get clear picturesin Live Cell Imaging.<br />
*Myell series are NOT sterilized<br />
Myell A/S: Prevent samples from vibrating><br />
When observing cells such as floating cellsor yeast, dropping cell suspension on a glass<br />
bottom dish is general method.<br />
However,this method has a problem that the picture becomes blurry because the cells vibrate<br />
due to their brownian motion.<br />
By wrapping with Myell A/S, the samples on aslide grass are prevented from vibrating. This<br />
makes usto get clear pictures.<br />
Also, pictures hardly blurry even if some droplets are added on samples wrapped with Myell<br />
A/S.<br />
Please click Here!<br />
https://fnkprddata.blob.core.windows.net/domestic/f-img/Image/TUN%<br />
20_myell_wrapping_54sec.mp4<br />
Product Name Code Size<br />
Poro<br />
us<br />
Myell A MYLL-A3 3 sheets -<br />
Myell S MYLL-S10 10 sheets -<br />
Material<br />
Polylactic acid<br />
Myell D MYLC-D10 10 sheets - CYTOP ®<br />
Myell P MYLL-P4 4 sheets 6µm Polylactic acid<br />
Enjoy our<br />
comprehensi<br />
ve<br />
PELOBiotech<br />
-Productlists<br />
MSCs, Tools<br />
& Media:<br />
www.yumpu.c<br />
om/en/<br />
embed/view/<br />
iApHInxFeXgG<br />
Vk2N<br />
Human Cells:<br />
www.yumpu.<br />
com/en/<br />
embed/view/<br />
Tt8iOdyNeG6<br />
RhD0I<br />
Animal Cells:<br />
www.yumpu.<br />
com/en/<br />
embed/view/<br />
KMxQalvUim<br />
o4SetV<br />
Papers, Papers, Papers<br />
A microfluidic model of human brain (μHuB) for assessment of BBB<br />
Microfluidic cellular models, commonly referred to as “organs-on-chips,” continue to advance the<br />
field of bioengineering via the development of accurate and higher throughput models, captivating<br />
the essence of living human organs.<br />
SynBBB in vitro blood brain barrier (BBB) model allows visualization of cellular interactions and barrier functionality<br />
in real time. Evaluate and quantitate permeability of therapeutics and small molecules across the endothelium<br />
of the BBB in real-time. Unlike BBB models that are membrane-based and arranged in top to bottom architecture<br />
(i.e., Transwell), small molecule transport can be visualized, accessed and quantified in real-time using the SynBBB<br />
model due to its side-by-side architecture.<br />
https://doi.org/10.1002/btm2.10126 by Tyler D. Brown et al
<strong>PELONews</strong><br />
How to<br />
reach us<br />
If you need any further<br />
assistance or if you<br />
like what you see, tell<br />
us :-):<br />
PELOBiotech GmbH<br />
Klopferspitz 19<br />
82152 Planegg |<br />
Germany<br />
Tel.:<br />
+49 89 517 286 59 0<br />
info@pelobiotech.com<br />
www.pelobiotech.com<br />
Managing Directors:<br />
Dr. Peter Frost,<br />
Dr. Lothar Steeb<br />
You like what you see?<br />
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