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PELONews Summer 2020

Read the latest edition of PELONews with PELOAcademy some topics are GMP, BBB, Dyes &Cell Imaging Reagents, Covid-19, 3D Cell Culture, cancer

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PELONEWS<br />

WITH FOCUS ON WELCOME TO LAB<br />

PELOBiotech Newsletter <strong>Summer</strong> <strong>2020</strong><br />

"Everything will be<br />

okay in the end.<br />

If it´s not okay it´s not<br />

the end.“<br />

- John Lennon - English singer<br />

& songwriter -<br />

Pic: Pixabay<br />

Dear Scientist,<br />

Yes, you can´t wait to get back to my bench, lab&office. Me too. So welcome<br />

everybody to óur new reality. We know that COVID-19 has impacted<br />

upon your research, so we like to help you get back on track as fast<br />

as possible. We are ready to support you in any kind you need.<br />

And we are as fast as always an d already updates our prices with the<br />

reduced tax rate in Germany. So no worries about that...<br />

If you would like to order your products now and hold delivery until you<br />

are able to return to the lab, please let us know upon ordering. We will<br />

be happy to assist you in smoothing the transition back to the bench<br />

whenever you are ready and deliver when you need us. Please check<br />

with your facility whether now there are changes in your logistics routine.<br />

Are you still accepting and getting deliveries all the times? We will<br />

check in with you before we send out your delivery as we ask you politely<br />

to do the same. Thank you.<br />

To discuss this our logistics, customer success&support are here for any<br />

of your questions. Online resources are available to teams around the<br />

world, so you can schedule an Online-Meeting and Support anytime via<br />

phone or Go-to-meeting. We are just one click away.<br />

On behalf of the global PELOBiotechTeam, we’re here for you.<br />

Take very good care and stay safe!<br />

Christiane Büchsel<br />

Editor in Chief <strong>PELONews</strong><br />

PS Also watch out for our online-attendence at GSCN - the German<br />

StemmCell Network. We love to meet you there.<br />

Content<br />

Products related to CO-<br />

VID-19 Research & Dyes<br />

PELOAcademy:<br />

Join us every week for<br />

new refreshing webinars<br />

and our new Series:<br />

Can Cell Culture be as<br />

good as in vivo today?<br />

2D/3D Cell culture: July 23<br />

NEW Products<br />

Watch a fresh Webinar:<br />

Dyes&Cell Imaging Reagents<br />

Our Portfolio<br />

See what we got


<strong>PELONews</strong><br />

<strong>PELONews</strong>: Cancer Research&COVID-19 List<br />

NEW Unique<br />

Imaging Reagents<br />

• Low toxic nucleus imaging<br />

dye = NucleoSeeing<br />

• Washable fluorescent dye<br />

for cytoplasm = CytoSeeing<br />

• Long term imaging fluorescent<br />

dye for ER = ERseeing<br />

• Highly sensitive fluorescent<br />

dye for Lipid<br />

Droplets = LipiDye<br />

• Fatty acid degradation<br />

(FAO) imaging reagent =<br />

FAOBlue<br />

• Intracellular temperature<br />

imaging dye = Thermoprobes<br />

NEW Overview Dyes & Cell Imaging Reagents<br />

As our customer, you can find<br />

all commercially available<br />

primary cells in our product<br />

range.<br />

PELOBIOTECH is your choice<br />

to fulfill all needs you actually<br />

have or that will come up<br />

during your project. PELOBIO-<br />

TECH combines long lasting<br />

expertise with high quality<br />

production methods in cells<br />

and media to build up a solid<br />

basis for your work. Be surprised<br />

what now is state of the art<br />

and clears your path to success.<br />

To get more infos please<br />

send an email to<br />

cellcultureexpert@pelobiotech.com<br />

subjectline<br />

„cell Imaging“.<br />

COVID-19 Research Products&Tools<br />

https://www.yumpu.com/en/document/read/63207661/human-primary-lung-cellslist-<strong>2020</strong><br />

MORE<br />

LUNG cells (Ace2 is the entry point of the Coronavirus in lung epithelial<br />

cells). Most primary Cells from Healty donors and diseased cells Smokers,<br />

Hypertension, Diabetes type 2, Asthma, COPD and more. Build your own<br />

assay with all these risk grouped . Also use our Cell Sourcing. Anti-SARS-2<br />

drug delvelopment.<br />

<br />

Lung endothelial cells microvasucular<br />

Download our special<br />

Covid19-product list<br />

here<br />

https://www.yumpu.com/<br />

en/embed/view/<br />

uEUNB4wVar3FCQ5n<br />

<br />

<br />

<br />

<br />

Lung fibroblasts<br />

Alveolar epithelial cells<br />

Tracheale bronchial smooth muscle cells<br />

Lung epithelial cells and more. See also page 6 for more new products.


PELOAcademy: Our Webinar-Series<br />

PELOAcademy<br />

3D Cell Culture-Series: 2D/3D Cell Culture<br />

presented by Dr. Peter Frost, CEO/CSO, PELOBiotech<br />

Thur July 23, 0220 10 AM German &3:30 PM (CET) English<br />

In the last 10 years Dr. Peter Frost was involved in 3D Cell Culture doing<br />

mainly business development for techniques like liquid overlay, magnetic<br />

spheroid formation, hanging drop technology and others. In the<br />

beginning it was a challenge to convince scientist to use 3D Cell Culture<br />

for their research and devlopments. 3D Cell Culture is nowadays more<br />

or less established in basic research, drug development, compound testing<br />

and personalized cancer diagnostics.<br />

PELOAcademy continues<br />

its series of Webinars:<br />

Can Cell Culture be as<br />

good as in vivo<br />

today? Roadmap to a<br />

better Worfkflow in<br />

your lab.<br />

We will discuss with you the<br />

most important steps during<br />

your workflow each 4th<br />

Thursday of the month in<br />

Today it is more the question and challenge to pick out the best possible<br />

3D cell culture technology for a scientists application or for your read<br />

out. This webinar will summarize many of the excisting techniques in<br />

context to different applications and you will get:<br />

- >Overview of excisting 3D techniques to perform 3D cell culture<br />

- >Advantages/Disadvantages of diferent techniques in context to some<br />

application<br />

-> Possible read out systems for 3D spheroids<br />

NEW Products - Unique Imaging<br />

Reagents and Advanced Cell Culture Tools<br />

Watch recording and get your overview presented by Funakoshi, Japan Join<br />

here: www.pelobiotech.com/pelo-academy<br />

German and English.<br />

No hassle. Watch your<br />

recording on demand.<br />

Part 1- The Informed<br />

Consent Part 2. Tissue<br />

Isolation & Cell Viability<br />

Part 3 Cell Seeding &<br />

Cell Requirements Part 4.<br />

Serum-&Xeno-free Cell<br />

Culture Media Part 5.<br />

2D&3D Cell Culture<br />

Coming soon:<br />

Tissue & Cell Sourcing,


<strong>PELONews</strong><br />

New Products & New Partners<br />

#3DCellCulture<br />

#HTS<br />

#spheroids<br />

TAGS<br />

3D CoSeedis comprises a<br />

family of chips suited for various<br />

amounts of 3D constructs.<br />

They range from 200 spheroids<br />

(3D CoSeedis Chip200) to<br />

880 spheroids per chip (3D<br />

CoSeedis Chip880), both in 6<br />

-well plate format, and also<br />

include a version for 680<br />

spheroids per chip (3D<br />

CoSeedis Chip680) for 24-<br />

well plates. Chips can either<br />

be purchased in single packages<br />

or in bulk.<br />

Download your copy of the<br />

paper here<br />

and contact us for further<br />

questions and information.<br />

NEW White paper by abc bioplly: How to<br />

Revolutionise the Use of 3D Cultures in HTS<br />

Our partner abc biopply has released a white paper demonstrating the unprecedented<br />

quality of 3D CoSeedis in the production of highly homogenous<br />

and uniform spheroids for the use in high-throughput and highcontent<br />

screening applications.<br />

The paper was done in collaboration with Union Biometrica, Inc. (USA) and<br />

shows how 3D CoSeedis can be used as a manufacturing tool to produce<br />

large quantities of homogenous and uniform 3D spheroids in a HTS/HCScompatible<br />

384-well format.<br />

The 3D CoSeedis<br />

platform thereby not only<br />

sets new standards in highvolume<br />

production of 3D<br />

spheroids but it also helps<br />

to substantially increase<br />

screening efficiency. It’s<br />

based on a 24-well plate<br />

format for aggregation,<br />

growth and harvesting and<br />

the resulting spheroid suspension<br />

is used to seed<br />

384-well plates with individual<br />

spheroids using Union Biometrica’s large particle sorter. The resulting<br />

384-well plates contain viable and uniform spheroids in more than 98% of<br />

all the wells and show minimal size variability from one plate to another.<br />

3D CoSeedis thereby helps to decrease screening failure rates and<br />

consequently increases overall screening efficiency<br />

NEW Media & New Partner for MSCs<br />

Cell culture is a critical component of research, development and production<br />

in the life sciences. Cell culture media in particular, has a significant impact on<br />

the efficacy and sensitivity control of the cell culture microenvironment.<br />

PELOBiotech is strenghtening its leading position in the field of MSCs & GMP:<br />

We added two media for MSCs to our portfolio called CellCor, a serum free<br />

chemically defined media composed of over 200 known compounds.<br />

Through 10 years of tenacious research, producer Xcell Therapeutics , South<br />

Korea, developed CellCor SFD MSC and CellCor CD MSC products, which<br />

provide superior performance with the upmost focus safety and quality, assured<br />

by strict adherence to regulated standards following good manufacturing<br />

practices. CellCor does not contain any animal nor plant derived components,<br />

they are hPL free, and Exosome free, available in R&D and GMP<br />

quality.


New Products<br />

Myell: Get clear pictures of Live Cell Imaging<br />

Myell series is a polymer "superthin" film "which is a novel alternative<br />

tool for cover glass" and offers a new method to make sample for microscope<br />

observation.<br />

By using with Myell series, the samples are prevented from vibrating. This<br />

makes us to get clear picturesin Live Cell Imaging.<br />

*Myell series are NOT sterilized<br />

Myell A/S: Prevent samples from vibrating><br />

When observing cells such as floating cellsor yeast, dropping cell suspension on a glass<br />

bottom dish is general method.<br />

However,this method has a problem that the picture becomes blurry because the cells vibrate<br />

due to their brownian motion.<br />

By wrapping with Myell A/S, the samples on aslide grass are prevented from vibrating. This<br />

makes usto get clear pictures.<br />

Also, pictures hardly blurry even if some droplets are added on samples wrapped with Myell<br />

A/S.<br />

Please click Here!<br />

https://fnkprddata.blob.core.windows.net/domestic/f-img/Image/TUN%<br />

20_myell_wrapping_54sec.mp4<br />

Product Name Code Size<br />

Poro<br />

us<br />

Myell A MYLL-A3 3 sheets -<br />

Myell S MYLL-S10 10 sheets -<br />

Material<br />

Polylactic acid<br />

Myell D MYLC-D10 10 sheets - CYTOP ®<br />

Myell P MYLL-P4 4 sheets 6µm Polylactic acid<br />

Enjoy our<br />

comprehensi<br />

ve<br />

PELOBiotech<br />

-Productlists<br />

MSCs, Tools<br />

& Media:<br />

www.yumpu.c<br />

om/en/<br />

embed/view/<br />

iApHInxFeXgG<br />

Vk2N<br />

Human Cells:<br />

www.yumpu.<br />

com/en/<br />

embed/view/<br />

Tt8iOdyNeG6<br />

RhD0I<br />

Animal Cells:<br />

www.yumpu.<br />

com/en/<br />

embed/view/<br />

KMxQalvUim<br />

o4SetV<br />

Papers, Papers, Papers<br />

A microfluidic model of human brain (μHuB) for assessment of BBB<br />

Microfluidic cellular models, commonly referred to as “organs-on-chips,” continue to advance the<br />

field of bioengineering via the development of accurate and higher throughput models, captivating<br />

the essence of living human organs.<br />

SynBBB in vitro blood brain barrier (BBB) model allows visualization of cellular interactions and barrier functionality<br />

in real time. Evaluate and quantitate permeability of therapeutics and small molecules across the endothelium<br />

of the BBB in real-time. Unlike BBB models that are membrane-based and arranged in top to bottom architecture<br />

(i.e., Transwell), small molecule transport can be visualized, accessed and quantified in real-time using the SynBBB<br />

model due to its side-by-side architecture.<br />

https://doi.org/10.1002/btm2.10126 by Tyler D. Brown et al


<strong>PELONews</strong><br />

How to<br />

reach us<br />

If you need any further<br />

assistance or if you<br />

like what you see, tell<br />

us :-):<br />

PELOBiotech GmbH<br />

Klopferspitz 19<br />

82152 Planegg |<br />

Germany<br />

Tel.:<br />

+49 89 517 286 59 0<br />

info@pelobiotech.com<br />

www.pelobiotech.com<br />

Managing Directors:<br />

Dr. Peter Frost,<br />

Dr. Lothar Steeb<br />

You like what you see?<br />

Sign up for <strong>PELONews</strong><br />

now at<br />

www.pelobiotech.com<br />

or just fill out the following<br />

form online.<br />

Fill out our Portfolio and send<br />

it back to us.<br />

info@pelobiotech.com<br />

Or give us a call…<br />

+49 89 517 286 59-0<br />

We like your feedback – tell us what you<br />

love, don’t like so much and what you<br />

would like to get, please. Just reply Please<br />

update your subscription anytime.

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