2020_Fall PeloNews

PELONEWS
WITH FOCUS ON MSCS AND ECMS
PELOBiotech Newsletter Fall 2020
"Logic will get you
from A to B.
Imagination will take
you everywhere.“
- Albert Einstein, German
physicist_
Dear Scientist,
Some are leaving, we are growing. So we like to say welcome on
board our new Sales-Team members Dr. Ingrid Kautner and Dr.
Gautier Arista. Just go to page 6 to learn more about them.
Pic: Pixabay
As we get more and more questions about how we will handlle the
Brexit: We continue to deliver to the UK, no worries, stay happy. And
as fast as we can in the same manner you are used from PELOBiotech.
The exact delivery conditions will depend on the regulations as
UK will no longer be part of the Europen Union. But we already have
very good experiences delivering to e.g. Switzerland and other No-
EU countries. So we do not expect any major challenges.
You don´t know how long you will be able to work on the bench,
lab&office? We know that COVID-19 has impacted upon your research,
so we like to help you stay on track as fast as possible. We are ready to
support you in any kind you need.
If you would like to order your products now and hold delivery until you
are sure you can go on, please let us know upon ordering. Please check
with your facility whether there have been changes in your logistics routine
due to new safety protocols:
Are you still accepting and getting deliveries the same way? We will
check in with you before we send out your delivery as we ask you politely
to do the same. Thank you.
To discuss this our logistics, customer success&support are here for any
of your questions. Online resources are available to teams around the
world, so you can schedule an Online-Meeting and Support anytime via
phone or Go-to-meeting. We are just one click away.
On behalf of the global PELOBiotechTeam, we’re here for you.
Take very good care and stay safe!
Christiane Büchsel
Editor in Chief PELONews
Content
Products related to CO-
VID-19 Research & Dyes
PELOAcademy:
Can Cell Culture be as
good as in vivo today?
Truly defined serum-free &
chemically defined media for
MSCs & Advanced Coatings
New White Paper: How to
Revolutionise the Use of 3D
Cultures in HTS
NEW Products
Special ECM by Rokit
Healthcare
Mini Cell Strainer
Our Portfolio: Fill out and
see what we got

PELONews
PELONews: Covid / Dyes
MORE
Download our special
Covid19-product list
here
https://www.yumpu.com/
en/embed/view/
uEUNB4wVar3FCQ5n
Get the right dye
for your research
• Low toxic nucleus imaging
dye = NucleoSeeing
• Washable fluorescent dye
for cytoplasm = CytoSeeing
• Long term imaging fluorescent
dye for ER = ERseeing
• Highly sensitive fluorescent
dye for Lipid
Droplets = LipiDye
• Fatty acid degradation
(FAO) imaging reagent =
FAOBlue
Intracellular temperature
imaging dye = Thermoprobes
To get more infos please
send an email to
cellcultureexpert@pelo
biotech.com subjectline „cell
Imaging“.
Get brochure ->
https://www.yumpu.com/kiosk/
pelobiotech/dyes-cell-imagingreagents-2020/63692006
COVID-19 Research Products&Tools
find out more about viral infection and pick your human cell from healthy donors
or a huge number of lung cells from diseased donors (Asthma, COPD, Diabetes,
Hypertension) and smokers for your research, drug development and drug testing.
Together with 3D techniques it is also possible to work with more predictive
in vitro systems.
~3D Cell Culture & Systems:
• Cytokine release syndrome/cytokine storm (CRS): 3D CoSeedis. See next page
• SynAIL Air Liquid Interface based Lung Model
• 3D Dynamic Cell Culture Models: LiveBox
• Lung Epithelial Models: 3-D-Airway model
~Primary cells plus corresponding media
• Endothelial cells
• Epithelial cell
• Smooth Muscle Cells
• Airway from lung, bronchia, trachea, alveoli, tracheal smooth
muscle, etc.
• Healthy and diseased donors, also from Smokers, cells are sorted by disease
• Customized Cells
Special: Industrial lots available. Reserve your badge now. One unique feature
of these cells: They are antibody free. Get a biologically relevant tool and a more
effective way to enhance insights.
~iPS cells
~Cell Culture Media & Reagents for Passaging
~Media for Cryopreservation
~Biomatrices & Coating Proteins for Cell Culture Application
~Tissue Specific Extracellular Biomaterials
~ Diagnostic antibodies:
• SARS-CoV-2 • ACE-2 • CD147 • IL1a • IL1b • IL6 • TMPRSS2 • TNFa

PELOAcademy: Our Webinar-Series
New Defined Serum-free and
Chemically Defined Media for MSCs
PELOAcademy
presented by Dr. Peter Frost, CEO, PELOBiotech
Date: Thur October 22, 2020
Time: 10 AM German & 3.30 PM English
Save ticket -> www.pelobiotech.com/pelo-academy
We discuss ou the most important steps during your
workflow. This time it will be about: Mesenchymal Stem
Cells (MSCs) are widely used in regenerative medicine; in
research as well as in clinical application. The in vitro
culture and expansion is done by mainly using Fetal Bovine
Serum (FBS) or human Platelet Lysate (hPL) based
media. Media containing hPL are very popular as this
facilitate a xeno-free culture of MSCs; in 2D as well as in
3D. As hPL is a human blood product safety issues are of
high priority and authorities ask for virus inactivated hPL
for clinical application. To overcome these safety issues,
defined serum-free and/or chemically defined media for
MSCs are needed. It is also necessary, for the production
and investigation of extracellular vesicles (EV) using primary
cells (e.g. MSCs), to have defined serum-free and/
or chemically defined media to prevent an EV contamination
by serum or hPL.
We present data using the defined serum-free (CellCorTM SFD MSC) and
chemically defined (CellCorTM CD MSC) media of our partner XCell
Therapeutics. We will show that these media can be used for MSCs culture
as well as for MSCs expansion; Also a short overview of the most
advanced Extracellular Matrices.
PELOAcademy continues
its series of Webinars:
Can Cell Culture be as
good as in vivo
today? Roadmap to a
better Worfkflow in y-
our lab. each 4th Thursday
of the month in Ger-
Product list
MSCs, Tools and Media
See what we got… Yes, it is so nice to work with great
products. Find out now…
https://www.yumpu.com/en/embed/view/
iApHInxFeXgGVk2N

PELONews
New Products & New Partners
#3DCellCulture
#HTS
#spheroids
TAGS
3D CoSeedis comprises a
family of chips suited for various
amounts of 3D constructs.
They range from 200 spheroids
(3D CoSeedis Chip200) to
880 spheroids per chip (3D
CoSeedis Chip880), both in 6
-well plate format, and also
include a version for 680
spheroids per chip (3D
CoSeedis Chip680) for 24-
well plates. Chips can either
be purchased in single packages
or in bulk.
Download your copy of the
paper here
and contact us for further
questions and information.
NEW White paper by abc biopply: How to
Revolutionise the Use of 3D Cultures in HTS
Our partner abc biopply has released a white paper demonstrating the unprecedented
quality of 3D CoSeedis in the production of highly homogenous
and uniform spheroids for the use in high-throughput and highcontent
screening applications.
The paper was done in collaboration with Union Biometrica, Inc. (USA) and
shows how 3D CoSeedis can be used as a manufacturing tool to produce
large quantities of homogenous and uniform 3D spheroids in a HTS/HCScompatible
384-well format.
The 3D CoSeedis
platform thereby not only
sets new standards in highvolume
production of 3D
spheroids but it also helps
to substantially increase
screening efficiency. It’s
based on a 24-well plate
format for aggregation,
growth and harvesting and
the resulting spheroid suspension
is used to seed
384-well plates with individual
spheroids using Union Biometrica’s large particle sorter. The resulting
384-well plates contain viable and uniform spheroids in more than 98% of
all the wells and show minimal size variability from one plate to another.
3D CoSeedis thereby helps to decrease screening failure rates and
consequently increases overall screening efficiency.
Papers, Papers, Papers
Mechanical communication of endothelial cells and
its influence on the organization of cellular structures
The formation of new blood vessels from existing ones is called angiogenesis and plays an important
role in tissue development, healing and homeostasis as well as in pathological processes, such as tumor
growth. During this process, coordinated cell movement is essential for which endothelial cells use a chemical communication
via the vascular endothelial growth factor (VEGF). The extracellular matrix (ECM) regulates many tissue
processes and influences cell behavior. The dynamic cell-ECM interaction enables a mechanical communication
between cells. This thesis aims to validate, whether endothelial cells also use a mechanical communication for
coordinated cell behavior. For the investigation of the endothelial … read more …..
https://edoc.ub.uni-muenchen.de/26600/index.html by Daniel Rüdiger, Jena. Congrats on this Diss!

New Products
NEW Partner Rokit Healthcare for Human Nativelike
Coating and Targeted Cell type Growth
PELOBiotech welcomes a new partner aboard: As of today we happily offer
HumaTein products from Rokit Healthcare (South Korea) in Europe. Cell Culture
Expert PELObiotech widens its extensive portfolio of specific matrices.
HumaTein composition is optimized for a target cell type growth and
proliferation in a native-like environment. The best possible Extracellular
Matrix (ECM) is most important for cell attachment, proliferation and migration,
and plays an import role as a growth factor reservoir.
Plus for Scientists:
Easy: HumaTein is not sensitive to temperature changes.
In-Vivo: Human primary cell derived HumaTein provides the most invivo
like environment to cells, great for translational studies
Specific: provides organ specific stiffness controlled environment
Native: preserves whole ECM components, so the is as human-like as
possible
HumaTein offers a wide spectum of applications:
Cancer Research
Stem Cell Research
Drug Discovery
Tissue Engineering & Regenerative Medicine
2D Cell Culture as Coating for primary cells or HUVEC migration assay
3D Cell Culture for e.g. Organoid Culture, Natural scaffold for inital cell
aggregation (iPS cell spheroids)
TAGS
#ECM
#PrimaryCells
#Cancer Organoids
#Matrigel
#Huvec
#DrugDiscovery
#3DCellCulture
#StemCells #MSCs #iPS
#scaffold
#hydrogel
Available Products
HumaTein TM Essential Powder Type
HumaTein TM Essential Powder Type
HumaTein TM MSC Powder Type
HumaTein TM MSC Powder Type
HumaTein TM Atello Collagen
A
It is also well known in the research for Wound Healing and Anti Aging.
HumaTein Matrix is an ideal coating material for feeder layer free induced
pluripotent stem cell (iPSC) culture. Xeno free and human cell derived Huma
Tein is optimized for organoid research.
HumaTein will be produced in cell culture using human dermal fibroblast or
BM MSC. The product is whole ECM derived by these cells together with the
expressed cytokines and growth factors. HumaTein consists of > 300 components
which incudes collagen, laminin, fibronectin, glycosaminoglycans,
more than 500 cytokines.
It is offered as powder produced with human Fibroblast (HumaTein Essential)
or BM MSC (HumaTein Msc) and the HumaTein Atello Collagen a hydrogel
which is not a 100 % human product.)
If you like to know more about this , please let us know and email to
info@pelobiotech.com

PELONews
TAGS
Cell Culture #Flow
Cytometry #FACS
Mini Cell Strainer
You need it?
We have it
Welcome aboard!
NEW Faces at PELOBiotech
Dr. Ingrid Kautner
has specialized in microbiology and
molecular biology with degrees from
Canada and Germany. After obtaining
her PhD she worked on dengue viruses
in Malaysia making her our most travelled
Account Manager. With her
profound knowledge of our portfolio
she will be able to offer advice on the
most suitable products for your projects.
How to
reach us
If you need any further
assistance or if you
like what you see, tell
us :-):
PELOBiotech GmbH
Klopferspitz 19
82152 Planegg |
Germany
Facilitate speedy cell separation
from primary tissues or
cell clumps
Slits on the rim avoid
overflow and help
speedy filtration
Fit for 5 ml sampling
and 15 ml conical tubes
Color-coded according
to mesh pore sizes (25
μm : White, 40 μm :
Blue, 70 μm : Clear)
Materials : Frame ; PP,
Mesh ; Nylon
EOG sterilized and individually
wrapped.
Good for speedy separation
for FACS. Ask for a sample.
Get quote here: ->
www.pelobiotech.com/?
s=mini+cell+strainer
Details here->
www.pelobiotech.com/wpcontent/uploads/2020/10/
HIT_170206_PB_MiniCellSt
rainer.pdf
„ I really love to be here at PELOBiotech, setting gears
in motion. I love the direct contact with my customers
and to find out what works best for their specific
projects“, says the native of Stuttgart who has made
Frankfurt her home from where she will serve customers in
postal code areas 3 and 5.
Email: ingrid.kautner@pelobiotech.com
P: +49 89 517 286 59-0
Dr. Gautier Arista
is our secret weapon for the French
speaking market. Together with our
on-site French Sales Team Michel
Vidal und Gilles Moraldi he will be
the first go to for our EU-wide
customers especially in Belgium,
Ireland, UK, Danmark, Slowenia and
Norway. He got his doctor in
Colmar, France, and was also in
Munich for postdocs.
Je m'appelle Gautier Arista et je viens agrandir la
famille PELOBiotech, votre expert pour les cellules, la
culture cellulaire et plus encore. Après un doctorat à
Colmar près de Strasbourg, je suis chargé des ventes internationales
à travers l'Europe et suis votre interlocuteur
pour questions techniques a PELOBiotech lorsque vous
désirez commander, si vous avez une question ou un problème.
N'hésitez pas à me contacter, en français ou anglais,
je reste à votre disposition.Vos contacts locaux en France
sont M. Michel Vidal (au nord) et M. Gilles Moraldi (au
sud). Ils attendent un appel à tout moment.
Email: gautier.arista@pelobiotech.com
P: +49 89 517 286 59-16
Tel.:
+49 89 517 286 59 0
info@pelobiotech.com
www.pelobiotech.com
Managing Directors:
Dr. Peter Frost,
Dr. Lothar Steeb
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Fax +49 89 517 286 59-88
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