Views
5 years ago

Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

Chapitre IV:

Chapitre IV: Functional Characterization of Tomato Hookless Genes plant species. Moreover, the size of exons was perfectly conserved between the two species. The size of Sl-HLS1 and Sl-HLS2 introns are quite different from those of At-HLS1 but the overall size of the tomato coding sequences is very close to that of At-HLS1 (1212 bp). Such high conservation of genomic structure is indicative of conserved function. A C WT hls1 hls1/ Sl-HLS2 WT hls1 hls1/ Sl-HLS2 B Hypocotyl length (mm) 18 16 14 12 10 8 6 4 2 0 WT hls1 hls1/Sl-HLS1 hls1/Sl-HLS2 Figure 2. Recovery of Hook Formation via Complementation of Arabidopdis hls1 Mutant with Sl-HLS1 and Sl-HLS2. Complemented lines display wild type apical hook in air-grown (A) and ethylene treated seedlings (C). Hypocotyl length of dark grown seedlings were measured at 4 days post-germination on air (B). The experiments were performed with homozygous transfomed lines and the data are mean ± SE of two independent replicates. Reversion of the Arabidopsis hls1 Mutant Phenotypes by Complementation with Sl-HLS1 and Sl-HLS2 Expresssion of Sl-HLS1 and Sl-HLS2 in hls1 Mutant Restores Normal Hook Formation To assess the functional significance of both tomato hookless genes, the arabidopsis hls1 mutant was transformed with Sl-HLS1 and Sl-HLS2 in sens 104

Chapitre IV: Functional Characterization of Tomato Hookless Genes orientation under the control of 35S cauliflower mosaic virus promoter. Among over twenty independent transgenic lines that display complemented phenotypes, three lines for each gene were used for further physiological analysis. In order not to increase the size of the figures the results corresponding to only one line are shown being representative of the two other lines. While no differential growth in the apical hook was observed in hls1 mutant, 3-day-old dark-grown seedlings transformed with either Sl-HLS1 or Sl-HLS2 develop an apical hook similar to that of wild type Arabidopsis plants both in absence or in presence of exogenous ethylene (Figure 2A, C). Since hls1 seedlings showed a reduced elongation of the hypocotyls in darkness, hypocotyl length was assessed in WT and complemented etiolated seedlings. The data presented in Figure 2B indicate that the reduced hypocotyl elongation in hls1 mutant is fully restored by ectopic expression of Sl- HLS1 and Sl-HLS2 tomato genes. Recovery of Normal Greening Phenotype of hls1 Mutant Complemented with Sl-HLS1 and Sl-HLS2 We showed previously that hls1 Arabidopsis seedlings that first and grown in the dark for several days and then transferred to white light undergo a marked bleaching leading to the plant death (Chapitre III). Complementation of hls1 WT hls1 hls1/ Sl-HLS2 Figure3. Recovery of the Greening Phenotype in Sl-HLS1 and Sl-HLS2 Complemented Lines. 4-day-old etiolated seedlings of wild type (WT), hls1 mutant (hls1) and Sl-HLS2 complemented (hls1/Sl-HLS2) lines were kept 48 hours on white light with a fluence rate (250 µmol.m -2 .s -2 ) on culture room. 105

Characterization of the subtilase gene family in tomato ...
CHARACTERIZING THE ROLES OF CELL POLARITY GENES ...
The diageotropica gene of tomato encodes a cyclophilin: a novel ...
Molecular identification and characterization of the tomato flagellin ...
Characterization of Tomatoes Expressing Anthocyanin in the Fruit
Evolution and Functional Characterization of the RH50 Gene from ...
Identification, Characterization, and Functional Analysis of a Gene ...
Identification and functional characterization of the Rad23 gene of ...
Identification and Characterization of Genes with Specific ...
Gene regulation in parthenocarpic tomato fruit - David Rocke
Molecular Identification and Functional Characterization of ...
Characterizations of the uro Mutant Suggest that the URO Gene Is ...
Characterization and functional role of voltage ... - Glucagon.com
Analysis and characterization of differential gene expression during ...
Stress-responsive a-dioxygenase expression in tomato roots
Functional Characterization of PaLAX1, a Putative - Plant Physiology
Determination and characterization of genes involved in toxic ...
Characterization of the role of COP9 signalosome in regulating ...
There is more to tomato fruit colour than candidate carotenoid genes
Characterization of Genes Related to Hyacinth Bulb Formation ...
Functional Characterization and Expression ... - Plant Physiology
Functional Characterization of OsMADS18, a Member of the AP1 ...
The role of auxin-signaling gene axr1 in salt stress and jasmonic ...
Elucidating the functional role of ... - Annals of Botany
1 Characterization and Functional Analysis of ABSCISIC ACID ...
characterization of novel genes predominantly expressed in ... - Doria
Role of STAT5a in regulation of sex-specific gene expression in ...
Roles of the NFI/CTF gene family in transcription and development
Regulation of Arabidopsis B function genes - Development - The ...