Views
5 years ago

Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

Chapitre IV:

Chapitre IV: Functional Characterization of Tomato Hookless Genes Sl-HLS1 and Sl-HLS2 Expression Restores Normal ABA Response Taking into account that hls1 mutantant is altered in ABA response, we tested the complemented lines for ABA-dependent developmental response. In the presence of 100 nM of ABA, 100% of hls1 mutant seeds underwent normal germination and subsequent growth while germination of WT seeds (18 % of germinated seeds) was strongly inhibited (Figure 5). Transgenic hls1 lines complemented with Sl-HLS1 and Sl-HLS2 genes, display even stronger inhibition of seed germination (10% of germinated seeds) than WT in the presence of ABA. These data confirm the implication of hookless genes in ABA signalling. Recovery of Normal Gravitropic Response of the hls1 Mutant Complemented with Sl- HLS1 and Sl-HLS2 We reported previously that both the root and the shoot of etiolated hls1 seedlings have agravitropic growth (Chaabouni et al. 2008, ChapitreIII). Expression of Sl-HLS1 and Sl-HLS2 in hls1 mutant was capable of restoring normal seedling gravitropism (Figure 6). Figure 6. Arabidopsis hls1 Mutant Complemented with Sl-HLS1 and Sl-HLS2 recover Normal Gravitropic Response. 3-day-old seedlings of wild type (WT), hls1 mutant (hls1) and hls1 complemented lines (hls1/Sl-HLS2) grown on MS media in the dark. Phenotypes Associated with Overexpression of Sl-HLS1 and Sl-HLS2 in wild type Arabidopsis To examine the effects of over-expression of the hookless gene on various developmental growth processes, wild type Arabidopsis plants were transformed 108

Chapitre IV: Functional Characterization of Tomato Hookless Genes with a tomato Sl-HLS1 and Sl-HLS2 genes under the control of 35S promoter. More than thirty independent transformed lines were identified for each gene that were cheked for their high level of Sl-HLS1 and Sl-HLS2 mRNA accululation (data not shown). Figure 7A shows that in the absence of ethylene, complemented etiolated seedling lines did not display exagerated apical hook curvature compared to wild type. We then established a dose response curve of A B percentage % % of of green green seedlings seedlings 100 80 60 40 20 0 100 80 60 40 20 WT Ethylene treatment (µL.L-1 ) 0 0.01 0.1 1 WT/Sl-HLS1 WT/Sl-HLS2 WT 85 87 WT/Sl-HLS1 WT/Sl-HLS2 WT WT/ Sl-HLS1 WT/ Sl-HLS2 52 WT WT/Sl-HLS1 42 17 17 18 0 2 3 4 5 6 7 8 incubation period (days in dark) Figure 7. Over-expression of Tomato Sl-HLS1 and Sl-HLS2 Genes in Wild type Arabidopsis Confers Hypersensitivity to Ethylene. A. The effect of ethylene on the apical hook curvature of overexpressed lines comaped to the wild type was estimated visually and a tree scale range were used: begining of hook formation, full hook, and exagerated hook. The data are mean of two independent experiments. B. A greening phenotype of seedlings overexpressing tomato hookless gene in Arabidopsis. Etiolated seedlings of WT and overexpressed tomato hookless plants were kept 48 hours on white light after being in the darkness for a number of days. Seedlings (n>50) were assessed as to whether they were green or still yellow. WT/Sl-HLS2 WT WT/Sl-HLS1 9 WT/Sl-HLS2 109

CHARACTERIZING THE ROLES OF CELL POLARITY GENES ...
The diageotropica gene of tomato encodes a cyclophilin: a novel ...
Characterization of the subtilase gene family in tomato ...
Characterization of Tomatoes Expressing Anthocyanin in the Fruit
Evolution and Functional Characterization of the RH50 Gene from ...
Molecular identification and characterization of the tomato flagellin ...
Identification and functional characterization of the Rad23 gene of ...
The expression of tomato prosystemin gene in ... - Rosa Rao Lab
The expression of tomato prosystemin gene in tobacco plants highly ...
Characterizations of the uro Mutant Suggest that the URO Gene Is ...
Molecular Identification and Functional Characterization of ...
Characterization and functional role of voltage ... - Glucagon.com
Identification and Characterization of Genes with Specific ...
Identification, Characterization, and Functional Analysis of a Gene ...
Analysis and characterization of differential gene expression during ...
Molecular Characterization and Gene Expression Profiling ... - CUSAT
Determination and characterization of genes involved in toxic ...
Stress-responsive a-dioxygenase expression in tomato roots
Functional Characterization of PaLAX1, a Putative - Plant Physiology
Molecular and functional characterization of ... - URGV - Inra
There is more to tomato fruit colour than candidate carotenoid genes
Functional Characterization and Expression ... - Plant Physiology
Functional Characterization of OsMADS18, a Member of the AP1 ...
Characterization of Genes Related to Hyacinth Bulb Formation ...
Characterization of transcription factor gene SNAC2 ... - NCPGR
Characterization of a NAC-like gene that regulates stamen ...
Molecular Characterization of Lal2, an SRK-Like Gene ... - Genetics
The role of auxin-signaling gene axr1 in salt stress and jasmonic ...
Elucidating the functional role of ... - Annals of Botany