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Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

Objectifs des travaux de

Objectifs des travaux de thèse Chapitre I. Bibliographic review TABLE OF CONTENTS I. Introduction…………………………………………………………………………………………….....1 II. Synthesis of IAA…………………………………………………………………………………………1 II. 1 Auxin conjugates………………………………………………………………………........2 II. 2 De novo synthesis………………………………………………………………………......3 II. 3 Desactivation…………………………………………………………………………….…..4 III. Auxin distribution and transport: the road network………..…………………………………………5 III.1 Update carriers……………………………………………………………………….……...5 III.2 The efflux complexe and the importance of vesicle cycling…………….……………….6 IV. Auxin perception and signalling……………………………………………………………………...7 IV.1 TIR1 the heart of auxin-signaling: perception and receptors………………………......7 IV.2 Signaling……………………………………………………………………………….......11 IV.2.a. Auxin induced transcripts…………………………………….............................11 IV.2.b. Auxin-reponsive-elemen Aux/RE and isolation of Auxin Response Factor…12 IV.2.c.Transcriptional control…………………………………………………..……..…...13 V. Homeostasis…………………………………………………………………………………………....15 VI. Role of auxin…………………………………………………………………………........................16 VI.1 Cell division and tissue culture…………………………………………………………...17 VI.2 Organ patterning……………………………………………………………………….…..17 VI.2.a Phyllotaxis…………………………………………………………………………....17 VI.2.b Root initiation and gravitropism……………………………………………….…...18 VI.3. Apical dominance and branching……………………………………………….……….18 VI.4.Tropisms....................................................................................................................19 VI.5.Adventitious rooting...................................................................................................20 VI.6.Fruit growth................................................................................................................20 VI.7. Herbicides………………………………………………………………………………..…22 VII. Interaction of auxin with other hormones…………………………………………………………..23 VII.1 Auxin and gibberellin (GA)…………………………………………………………….... 24 VII.2 Auxin and jasmonic acid (JA)…………………………………………………………. .26 VII.3 Auxin and cytokinin (CK)………………………………………………………………... 27 VII.4 Auxin and abscisic acid (ABA)……………………………………………………...…. .28 VII.5 Auxin and brassinosteroids (BR)……………………………………………………….. 29 VII.6 Auxin and ethylene………... ……………………………………………………………..30 VII.6.a Multilevel interaction between auxin and ethylene in the roots……………... 33 VII.6.b Interaction between auxin and ethylene in the hook……………………….… 33 VII.7 Other gene bridge auxin response with other stimulus…………………………..……38 VII.7.a Auxin and light-dependant growth………………………………........................ 38 VII.7.b Auxin and photomorphogenesis………………………………………………... . 38 VIII. Tomato as plant model ……………..………………………………………………….…………...40 VIII.1 Fruit as an important component in the human fruit diet……………………………. 40 VIII.2 Tomato as a model system for fruit ripening………………………………………..... 41 VIII.3 The sequencing of the tomato genome…………………………………………..…… 41 VIII.4 Microtom: characteristics of miniature tomato………………………………………....42

VIII.5 Tomato plant characteristics………………………………………………………….... 43 VIII.6 Tomato fruit development………………………………………………………..…...….44 VIII.7 Tomato ripening…………………………………………………………………………...45 VIII.7a Revealing the secrets of ripening mutant…………………………………...….....47 VIII.7b Potential role on auxin in fruit development and ripening…………………...…..48 Chapitre II. The Sl-IAA3 tomato Aux/IAA gene is in the cross-roads of auxin and ethylene signalling Abstract…………………………………………………………………………………………………....50 Introduction…………………………………………………………………………………………….......51 Results……………………………………………………………………………………………………...53 Discussion………………………………………………………………………………………..….……..70 Methods…………………………………………………………………………………………..………...76 Supplemental data…………………………………………………………………………………..........81 References…………………………………………………………………………………………...........84 Chapitre III. HOOKLESS1, an integrator of multiple signaling pathways, involved in glucose and de-etiolation toxicity/sensitivity in Arabidopsis Abstract…………………………………………………………………………………………………....88 Introduction…………………………………………………………………………………………...........89 Results……………………………………………………………………………………………..............90 Discussion……………………………………………………………………………………...…….........94 Methods…………………………………………………………………………………………...……..…98 References……………………………………………………………………………………..................98 Chapitre IV. Isolation and functional characterization of tomato hookless genes by complementation of Arabidopsis hookless1 mutant Abstract………………………………………………………………………………………………..…101 Introduction………………………………………………………………………………………............102 Results……………………………………………………………………………………………............103 Discussion……………………………………………………………………………..…………….…...114 Methods…………………………………………………………………………………………….……..117 References………………………………………………………………………………………………..120 Conclusions et perspecvtives……………………………………………………………………….122 Bibliographic references……………………………………………………………..…………..…. 125

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