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Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

CHAPITRE II

CHAPITRE II Sl-IAA3, a Tomato Aux/IAA at the Crossroads of Auxin and Ethylene Signalling (Manuscript submitted)

Chapitre II: Sl-IAA3, a Tomato Aux/IAA at the Crossroads of Auxin and Ethylene Signalling Title: Sl-IAA3, a Tomato Aux/IAA at the Crossroads of Auxin and Ethylene Signalling Authors: Salma Chaabouni, Corinne Delalande, Brian Jones, Hua Wang, Zhengguo Li, Isabelle Mila, Pierre Frasse, Alain Latché, Jean-Claude Pech, and Mondher Bouzayen ABSTRACT Whereas the interplay of multiple hormones is essential for most plant developmental processes, the key integrating molecular players remain largely undiscovered or uncharacterized. We report here that Sl-IAA3, a member of the tomato auxin/indole-3-acetic acid (Aux/IAA) gene family, is an intersection point between the auxin and ethylene signal transduction pathways. Aux/IAA genes encode short-lived transcriptional regulators that mediate auxin responses. Their functions have mostly been defined by dominant, gain-of-function mutant alleles in Arabidopsis. The Sl-IAA3 gene encodes a nuclear-targeted protein that can repress transcription from auxin-responsive promoters. Sl-IAA3 expression is controlled by both auxin and ethylene and is regulated on a tight tissue-specific basis. Sl-IAA3 displays an expression gradient associated with tissues undergoing differential growth. In ethylene-treated etiolated seedlings, the expression of Sl-IAA3 is restricted to the inner side of the apical hook opposite to that of the tomato HOOKLESS gene. Down-regulation of Sl-IAA3 via an antisense strategy results in auxin and ethylene-related phenotypes including altered apical dominance, lower auxin sensitivity, exaggerated apical hook curvature in the dark and reduced petiole epinasty in the light. The ethylene-related phenotypes in the antisense tomato lines (AS-IAA3) reveal new roles for Aux/IAAs genes and position Sl-IAA3 firmly at the crossroads between auxin and ethylene signalling in tomato. 50

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