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Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

Chapitre II:

Chapitre II: Sl-IAA3, a Tomato Aux/IAA at the Crossroads of Auxin and Ethylene Signalling Supplemental Figure 5. Supplemental Figure 5. Reversion of the Arabidopsis hls1 Mutant Phenotypes by Complementation with the Sl-HLS tomato hookless gene. Ectopic expresssion of Sl- HLS in hls1 mutant restores the normal hook formation in 3-day-old etiolated seedlings treated with 1µL L -1 ethylene. Right panel: wild type (WT); middle panel: hookless mutant (hls1); left panel: hookless mutant expressing the tomato Sl-HLS gene (hls1/Sl-HLS). Supplemental tables Table 1. Percentage Identity of the Antisense Region Relative to the other Members of Tomato Aux/IAAs Family. Target genes Sl-AA3 SGN-U323670 SGN-U316052 SGN-U323974 SGN-U318434 SGN-U317606 SGN-U332300 SGN-U330168 SGN-U322175 SGN-U318191 SGN-U313802 SGN-U320280 SGN-U322787 SGN-U320412 SGN-U322644 SGN-U322499 SGN-U320261 % of identity 79 74 67 65 65 64 64 64 63 63 62 59 56 56 54 54 100 83

Chapitre II: Sl-IAA3, a Tomato Aux/IAA at the Crossroads of Auxin and Ethylene Signalling REFERENCES Abel, S., and Theologis, A. (1994). Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression. Plant J. 5: 421-427. Abel, S., Nguyen, M.D., Chow, W., and Theologis, A. (1995). ACS4, a primary indole acetic acid-responsive gene encoding 1-aminocyclopropane- 1-carboxylate synthase in Arabidopsis thaliana. Structural Ethylene–Auxin Interactions, characterization, expression in Escherichia coli, and expression characteristics in response to auxin. J. Biol. Chem. 270: 19093–19099. Erratum. J. Biol. Chem. 270: 26020. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389–3402. Bleecker, A.B., Estelle, M.A., Somerville, C., and Kende, H. (1988). Insensitivity to ethylene conferred by a dominant mutation in Arabidopsis thaliana. Science 26: 1086-1089. Chae, H.S., Cho, Y.G., Park, M.Y., Lee, M.C., Eun, M.Y., Kang, B.G., and Kim, W.T. (2000). Hormonal cross-talk between auxin and ethylene differentially regulates the expression of two members of the 1-aminocyclopropane-1-carboxylate oxidase gene family in rice (Oryza sativa L). Plant Cell Physiol. 41: 354-362. Darwin, C., and Darwin, F. (1896). The Power of Movement in Plants (New York: D. Appleton and Co.), pp. 87-94. Dharmasiri, N., and Estelle, M. (2004). Auxin signaling and regulated protein degradation. Trends Plant Sci. 9: 302-308. Dharmasiri, N., Dharmasiri, S., and Estelle, M. (2005a). The F-box protein TIR1 is an auxin receptor. Nature 435: 441-445. Dharmasiri, N., Dharmasiri, S., Weijers, D., Lechner, E., Yamada, M., Hobbie, L., Ehrismann, J.S., Jürgens, G., and Estelle, M. (2005b). Plant development is regulated by a family of auxin receptor F box proteins. Dev. Cell 9: 109-119. Ecker, J.R. (1995). The ethylene signal transduction pathway in plants. Science 268: 667-675. Gray, W.M., Kepinski, S., Rouse, D., Leyser, O., and Estelle, M. (2001). Auxin regulates SCF(TIR1)-dependent degradation of AUX/IAA proteins. Nature 414: 271-276. Guilfoyle, T.J, and Hagen, G. (2001). Auxin response factors. J. Plant Growth Regul. 20: 281- 291. Guilfoyle, T.J., and Hagen, G. (2007). Auxin response factors. Curr. Opi. Plant Biol. 10: 453-460. Guzman, P., and Ecker, J.R. (1990). Exploiting the triple response to identify ethylene-related mutants. Plant Cell 2: 513-523. Hagen, G., and Guilfoyle, T. (2002). Auxin-responsive gene expression: genes, promoters and regulatory factors. Plant Mol. Biol. 49: 373-385. Hellens, R.P., Edwards A.E., Leyland, N.R., Bean, S., and Mullineaux, P. (2000). pGreen: A versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol. Biol. 42: 819-832. 84

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