Views
5 years ago

Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

Functional characterization of tomato Sl-IAA3 and Sl-hls genes. Role ...

Chapitre II:

Chapitre II: Sl-IAA3, a Tomato Aux/IAA at the Crossroads of Auxin and Ethylene Signalling Supplemental Figure 5. Supplemental Figure 5. Reversion of the Arabidopsis hls1 Mutant Phenotypes by Complementation with the Sl-HLS tomato hookless gene. Ectopic expresssion of Sl- HLS in hls1 mutant restores the normal hook formation in 3-day-old etiolated seedlings treated with 1µL L -1 ethylene. Right panel: wild type (WT); middle panel: hookless mutant (hls1); left panel: hookless mutant expressing the tomato Sl-HLS gene (hls1/Sl-HLS). Supplemental tables Table 1. Percentage Identity of the Antisense Region Relative to the other Members of Tomato Aux/IAAs Family. Target genes Sl-AA3 SGN-U323670 SGN-U316052 SGN-U323974 SGN-U318434 SGN-U317606 SGN-U332300 SGN-U330168 SGN-U322175 SGN-U318191 SGN-U313802 SGN-U320280 SGN-U322787 SGN-U320412 SGN-U322644 SGN-U322499 SGN-U320261 % of identity 79 74 67 65 65 64 64 64 63 63 62 59 56 56 54 54 100 83

Chapitre II: Sl-IAA3, a Tomato Aux/IAA at the Crossroads of Auxin and Ethylene Signalling REFERENCES Abel, S., and Theologis, A. (1994). Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression. Plant J. 5: 421-427. Abel, S., Nguyen, M.D., Chow, W., and Theologis, A. (1995). ACS4, a primary indole acetic acid-responsive gene encoding 1-aminocyclopropane- 1-carboxylate synthase in Arabidopsis thaliana. Structural Ethylene–Auxin Interactions, characterization, expression in Escherichia coli, and expression characteristics in response to auxin. J. Biol. Chem. 270: 19093–19099. Erratum. J. Biol. Chem. 270: 26020. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389–3402. Bleecker, A.B., Estelle, M.A., Somerville, C., and Kende, H. (1988). Insensitivity to ethylene conferred by a dominant mutation in Arabidopsis thaliana. Science 26: 1086-1089. Chae, H.S., Cho, Y.G., Park, M.Y., Lee, M.C., Eun, M.Y., Kang, B.G., and Kim, W.T. (2000). Hormonal cross-talk between auxin and ethylene differentially regulates the expression of two members of the 1-aminocyclopropane-1-carboxylate oxidase gene family in rice (Oryza sativa L). Plant Cell Physiol. 41: 354-362. Darwin, C., and Darwin, F. (1896). The Power of Movement in Plants (New York: D. Appleton and Co.), pp. 87-94. Dharmasiri, N., and Estelle, M. (2004). Auxin signaling and regulated protein degradation. Trends Plant Sci. 9: 302-308. Dharmasiri, N., Dharmasiri, S., and Estelle, M. (2005a). The F-box protein TIR1 is an auxin receptor. Nature 435: 441-445. Dharmasiri, N., Dharmasiri, S., Weijers, D., Lechner, E., Yamada, M., Hobbie, L., Ehrismann, J.S., Jürgens, G., and Estelle, M. (2005b). Plant development is regulated by a family of auxin receptor F box proteins. Dev. Cell 9: 109-119. Ecker, J.R. (1995). The ethylene signal transduction pathway in plants. Science 268: 667-675. Gray, W.M., Kepinski, S., Rouse, D., Leyser, O., and Estelle, M. (2001). Auxin regulates SCF(TIR1)-dependent degradation of AUX/IAA proteins. Nature 414: 271-276. Guilfoyle, T.J, and Hagen, G. (2001). Auxin response factors. J. Plant Growth Regul. 20: 281- 291. Guilfoyle, T.J., and Hagen, G. (2007). Auxin response factors. Curr. Opi. Plant Biol. 10: 453-460. Guzman, P., and Ecker, J.R. (1990). Exploiting the triple response to identify ethylene-related mutants. Plant Cell 2: 513-523. Hagen, G., and Guilfoyle, T. (2002). Auxin-responsive gene expression: genes, promoters and regulatory factors. Plant Mol. Biol. 49: 373-385. Hellens, R.P., Edwards A.E., Leyland, N.R., Bean, S., and Mullineaux, P. (2000). pGreen: A versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol. Biol. 42: 819-832. 84

Characterization of the subtilase gene family in tomato ...
CHARACTERIZING THE ROLES OF CELL POLARITY GENES ...
The diageotropica gene of tomato encodes a cyclophilin: a novel ...
Characterization of Tomatoes Expressing Anthocyanin in the Fruit
Molecular identification and characterization of the tomato flagellin ...
Functional Characterization of OsMADS18, a Member of the AP1 ...
Functional Characterization and Expression ... - Plant Physiology
Evolution and Functional Characterization of the RH50 Gene from ...
Genetic and physiological characterization of tomato cv. Micro-Tom
Identification and functional characterization of the Rad23 gene of ...
Identification, Characterization, and Functional Analysis of a Gene ...
The expression of tomato prosystemin gene in ... - Rosa Rao Lab
The expression of tomato prosystemin gene in tobacco plants highly ...
Subunit Antisense Gene Expression in Tomato Plants Leads to a ...
Identification and Characterization of Genes with Specific ...
Gene regulation in parthenocarpic tomato fruit - David Rocke
Characterizations of the uro Mutant Suggest that the URO Gene Is ...
Characterization and expression of cytokinin signalling genes in ...
Identification and characterization of homeobox genes in ... - SciELO
Characterization and functional role of voltage ... - Glucagon.com
Molecular Identification and Functional Characterization of ...
Functional characterization of the three genes encoding 1-deoxy-D ...
Determination and characterization of genes involved in toxic ...
Molecular Characterization and Gene Expression Profiling ... - CUSAT
Stress-responsive a-dioxygenase expression in tomato roots
Analysis and characterization of differential gene expression during ...
Functional Characterization of PaLAX1, a Putative - Plant Physiology
Functional Characterization of Arabidopsis thaliana WRKY39 in ...
Molecular and functional characterization of ... - URGV - Inra