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Flower development of Lilium longiflorum - The Lilium information ...

Flower development of Lilium longiflorum - The Lilium information ...

Chapter 4 Sambrook J,

Chapter 4 Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2 nd ed. Cold Spring Harbor Laboratory Press, Plainview, NY Somerville C, Koornneef M (2002) A fortunate choice: the history of Arabidopsis as a model plant. Nat Rev Genet 3: 883-889 Tzeng, T-Y, Chen H-Y, Yang C-H (2002) Ectopic expression of carpel-specific MADS box genes from lily and lisianthus causes similar homeotic conversion of sepal and petal in Arabidopsis. Plant Physiol 130: 1827-1836 Tzeng T-Y, Yang C-H (2001) A MADS box gene from lily (Lilium longiflorum) is sufficient to generate dominant negative mutation by interacting with PISTILLATA (PI) in Arabidopsis thaliana. Plant Cell Physiol 42: 1156-1168 van Engelen FA, Molthoff JW, Conner AJ, Nap JP, Pereira A, Stiekema WJ (1995) pBINPLUS: an improved plant transformation vector based on pBIN19. Transgenic Res 4: 288-290 66

Transformation of Lilium via particle bombardment CHAPTER FIVE Transformation of Lilium longiflorum via particle bombardment and generation of herbicide-resistance plants Vagner Augusto Benedito, Bernadette C. E. van Kronenburg-van de Ven, Abstract Jaap M. van Tuyl, Gerco C. Angenent, Frans A. Krens Plant Research International Wageningen University and Research Center, P.O. Box 16, 6700AA Wageningen, the Netherlands; This work is a contribution towards the improvement of lily (Lilium longiflorum Thunb.) transformation procedures. A vector carrying the Arabidopsis SUPERMAN gene driven by the petunia flower-specific FLORAL BINDING PROTEIN 1 promoter and the bialaphos resistance gene phosphinothricin acetyltransferase under the CaMV35S promoter was used to transform bulblet slices by particle bombardment. Our intentions were improving the transformation parameters for lily transformation in order to reach higher efficiency, and creating novel phenotypes in lily flowers using transcription factors originating from dicot plants. We were capable of obtaining transgenic lines expressing in vitro resistance to bialaphos. The transgenic plants were transferred to the greenhouse, grown and monitored for two flowering seasons. Flowers derived from these plants appeared normal and indistinguishable from wild- type flowers and the possible reasons for this are currently under investigation. 67

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