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Copyright by Eunyoung Park 2007 - The University of Texas at Austin

Copyright by Eunyoung Park 2007 - The University of Texas at Austin

FIGURE 4.2. RETINOL DOES

FIGURE 4.2. RETINOL DOES NOT CHANGE P110 OR P85 PROTEIN LEVELS OR P85/P110 HETERODIMERIZATION. 91

FIGURE 4.2. RETINOL DOES NOT CHANGE P110 OR P85 PROTEIN LEVELS OR P85/P110 HETERODIMERIZATION. Total protein was isolated from HCT-116 (A and B) and SW620 (D and E) cells treated with 0 (vehicle control), 1, or 10 μM retinol for 30 to 240 min after 24 h of serum starvation. p85 and p110 levels were detected using p85 and p110 antibodies as described in the Materials and Methods section. β-Actin was used as an internal loading control. Total protein was isolated from HCT-116 (C) and SW620 (F) cells treated with 0 (vehicle control) or 10 μM retinol for 5 to 120 min after 24 h of serum starvation. Total protein was immunoprecipitated using p110 antibody and sepharose 4A beads and detected using p85 antibody as described in the Materials and Methods section. Immunoprecipitated p110 was detected using p110 antibody and served as an internal loading control. Data are reported as mean ± SEM, of three independent experiments. 92

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