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Copyright by Eunyoung Park 2007 - The University of Texas at Austin

Copyright by Eunyoung Park 2007 - The University of Texas at Austin

FIGURE 5.1. AVERAGE FOOD

FIGURE 5.1. AVERAGE FOOD INTAKE AND BODY WEIGHT. Average body weight for 5 weeks on vitamin A supplemented diets of the chemotherapy study (A) and for 9 weeks on diets of the chemoprevention study (B). Average food intake for the chemotherapy study (C) and the chemoprevention study (D). Mice were fed with 2,400 (♦), 12,000 (▲), 25,000 (●), 50,000 (X), 100,000 (*), and 200,000 (�) IU vitamin A/kg diet in the chemotherapy and 2,400 (♦), and 200,000 (�) IU vitamin A/kg diet in the chemoprevention sutdy. Data shown are mean ± SEM [n=15 (A and B) and n=14 (C and D)]. *Significantly different from control (P < 0.05). 113

FIGURE 5.2. DIETARY VITAMIN A SUPPLEMENTATION DECREASES METASTATIC INCIDENCE; CHEMOTHERAPY DIETARY MODEL. Ninety female BALB/cAnNCrnu/nu mice, aged 6 to 8 wks, were intersplenically injected with 2 x 10 6 HCT-116 cells in 50 μl of magnesium and calcium free HBSS. Immediately following surgery the mice were divided into six groups (n=15 per group) and consumed diets containing increasing amounts of vitamin A as retinyl palmitate. Five weeks following surgery, all mice were sacrificed and the incidence of liver metastases assessed by cytokeratin 20 staining, (A) Negative (from normal liver) and (B) positive (liver metastases) CK staining. Dietary supplementation with 50,000, 100,000 or 200,000 IU vitamin A/kg diet decreased the incidence of hepatic metastasis (C). Data shown are % of mice per group exhibiting metastasis. 114

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