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Copyright by Eunyoung Park 2007 - The University of Texas at Austin

Copyright by Eunyoung Park 2007 - The University of Texas at Austin

FIGURE 2.3. RETINOL DOES

FIGURE 2.3. RETINOL DOES NOT INDUCE RARE-CAT REPORTER GENE EXPRESSION. HCT-15 (A), HCT-116 (B), SW620 (C), and WiDr (D) cells were transiently transfected with 1 μg pRARE-CAT and 0.5 μg pSV-β-gal using lipofectamine 2000. Twenty-four hours following transfection, cells were treated with 0, 1, and 10 μM RA or retinol. Cells were harvested 24 and 48 h after treatment and CAT and βgalactosidase assays were performed. CAT activity was normalized for transfection efficiency using the β-galactosidase activity. The CAT activity in control cells treated with the ethanol vehicle was set equal to one and all other values are expressed as fold induction. Values shown are the mean ± SE of three separate experiments. 35

FIGURE 2.4. RETINOL DOES NOT ACT THROUGH RAR TO INHIBIT CELL GROWTH. HCT-15 (A) cells were plated with 0 or 1 μM RA or retinol with or without 10 μmol/L of the RAR pan-antagonist, AGN 193109, for 48 h. HCT-116 (B), SW620 (C), and WiDr (D) were treated with 0 or 1 μM retinol with or without 10 μM AGN 193109 for 96 h. All treatments were plated in duplicate. Data shown are the mean of two separate experiments ± SE. 36

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