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Copyright by Eunyoung Park 2007 - The University of Texas at Austin

Copyright by Eunyoung Park 2007 - The University of Texas at Austin

FIGURE 2.5. RETINOL DOES

FIGURE 2.5. RETINOL DOES NOT INDUCE APOPTOSIS OR CELLULAR DIFFERENTIATION IN ATRA-RESISTANT HUMAN COLON CANCER CELL LINES. 37

FIGURE 2.5. RETINOL DOES NOT INDUCE APOPTOSIS OR CELLULAR DIFFERENTIATION IN ATRA-RESISTANT HUMAN COLON CANCER CELL LINES. HCT-15 (A), HCT-116 (B), SW620 (C), and WiDr (D) cells were plated and treated with 0, 1 or 10 μM retinol as described in Materials and Methods. To measure apoptosis (left column), floating and adherent cells were harvested every 24 hr, centrifuged and washed with PBS to remove all traces of media. Cells were stained with 2 μg/mL DAPI for 10 min in 37ºC before observation. To measure cellular differentiation (right column), cells were plated as described and treated with 0, 1, or 10 μM retinol or 2 mM sodium butyrate for 96 h. Alkaline phosphatase activity was determined as described (Turowski) by the conversion of p-nitrophenyl phosphate to p-nitrophenol at 410 nm. Alkaline phosphatase enzyme activity is expressed as percent positive control. Data shown are the mean ± SE for three independent experiments. 38

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