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Copyright by Eunyoung Park 2007 - The University of Texas at Austin

Copyright by Eunyoung Park 2007 - The University of Texas at Austin

FIGURE 2.6. RETINOL

FIGURE 2.6. RETINOL ALTERS CELL CYCLE PROGRESSION BUT DOES NOT INDUCE APOPTOSIS. 39

FIGURE 2.6. RETINOL ALTERS CELL CYCLE PROGRESSION BUT DOES NOT INDUCE APOPTOSIS. HCT-15 (A), HCT-116 (B), SW620 (C), and WiDr (D) cells were synchronized with serum starvation for a total of 48 h and the addition of aphidicholin during the last 24 h of serum starvation. Following synchronization, fresh medium containing 10 % FCS and 0 (left column) or 10 μM retinol (right column) was added for 24 h, prior to fixation and staining with propidium iodide. At least 10,000 cells were analyzed per sample using a FACS Calibur machine. Absence of a sub-G0/1 indicates that apoptosis was not induced by retinol treatment. Dark gray, G0/1 and G2/M; hatched, S-phase. One representative experiment of two is shown. 40

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