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Copyright by Eunyoung Park 2007 - The University of Texas at Austin

Copyright by Eunyoung Park 2007 - The University of Texas at Austin

FIGURE 3.2. EFFECT OF

FIGURE 3.2. EFFECT OF CO-TREATMENT WITH RETINOL AND CYCLOHEXIMIDE, ACTINOMYCIN D OR A PAN-RAR ANTAGONIST ON ATRA-RESISTANT COLON CANCER CELL INVASION. HCT-116 (left column) and SW620 (right column) cells were serum starved for 48 h before seeding at a density of 1 x 10 5 cells/well on Matrigelcoated Boyden chambers. The upper portion of the chambers contained 0 (ethanol vehicle control), 0.1, 1, or 10 μM retinol with or without 10 μg/ml cycloheximide (CHX) (A) or 2 μg/ml actinomycin D (Act D) (B). Cells were also treated with 1 μM retinol with and without 10 μM AGN193109 (C). The lower chamber contained 20 ng/ml hepatic growth factor and 10% FBS. Cell invasion was measured after 24 h by propidium iodide staining as described in Materials and Methods. All data are reported as mean ± SEM, n=3. *Significantly different from cells not treated with actinomycin D, P < 0.05. 63

FIGURE 3.3. EFFECT OF RETINOL ON MMP MRNA LEVELS. 64

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