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Copyright by Eunyoung Park 2007 - The University of Texas at Austin

Copyright by Eunyoung Park 2007 - The University of Texas at Austin

CHAPTER 4: RETINOL

CHAPTER 4: RETINOL DECREASES PHOSPHATIDYLINOSITOL 3-KINASE ACTIVITY IN COLON CANCER CELLS 69 ABSTRACT................................................................................................. 69 INTRODUCTION ....................................................................................... 70 MATERIALS AND METHODS................................................................. 72 RESULTS .................................................................................................... 78 DISCUSSION.............................................................................................. 85 ACKNOWLEDGEMENTS......................................................................... 89 CHAPTER 5: DIETARY VITAMIN A DECREASES LIVER METASTASES OF COLON CANCER IN MICE 98 ABSTRACT................................................................................................. 98 INTRODUCTION ....................................................................................... 99 MATERIALS AND METHODS............................................................... 101 RESULTS .................................................................................................. 104 DISCUSSION............................................................................................ 106 ACKNOWLEDGEMENT ......................................................................... 110 CHAPTER 6: SUMMARY AND FUTURE DIRECTIONS 117 REFERENCES 122 VITA 135 viii

List of Tables TABLE 3.1. SEQUENCE OF PRIMERS USED FOR SYBG QUANTITATIVE REAL TIME-PCR 61 TABLE 5.1. DIET COMPOSITION 111 TABLE 5.2. DIETARY VITAMIN A CONTENT VERSUS INCIDENCE OF METASTASES 112 ix

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