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Development of Real-Time Multiplex NASBA® for the - Journal of ...

Development of Real-Time Multiplex NASBA® for the - Journal of ...

480 Table 1. Bacterial

480 Table 1. Bacterial species and strains used to test the specificity of the assay NO.. Strain / Source Species Origin 1 ATCC VR-1355 Chlamydophila pneumoniae ATCC 2 Clinical isolate Chlamydia psittaci UZA 3 Kiq10 Chlamydia trachomatis ITG a 4 ATCC 29085 (PI1428) Mycoplasma pneumoniae type 1 ATCC b 5 ATCC 15492 (MAC) Mycoplasma pneumoniae type 2 ATCC 6 NC 10117 Mycoplasma fermentans NTCC c 7 NC10111 Mycoplasma hominis NCTC 8 ATCC 33530 (G-37) Mycoplasma genitalium ATCC 9 NC10112 Mycoplasma orale NCTC 10 NC10136 Mycoplasma buccale NCTC 11 NC10113 Mycoplasma salivarium NCTC 12 NC11702 Mycoplasma pirum NCTC 13 CDC BC 1636 Legionella pneumophila 1 UZA d 14 CDC BC 1637 Legionella pneumophila 2 UZA 15 Clinical isolate Legionella pneumophila 3 SEH e 16 strain Los Angeles Legionella pneumophila 4 UZA 17 strain Dallas Legionella pneumophila 5 UZA 18 Clinical isolate Legionella pneumophila 6 UZA 19 strain Concor Legionella pneumophila 8 UZA 20 Clinical isolate Legionella pneumophila 10 SEH 21 Clinical isolate Legionella. longbeachae 4a UZA 22 Clinical isolate Legionella. longbeachae 4b UZA 23 Clinical isolate Legionella bozemanni SEH 24 Clinical isolate Legionella micdadei SEH 25 Clinical isolate Ureaplasma urealyticum UZA 26 Clinical isolate Moraxella catarrhalis UZA 27 Clinical isolate Haemophilus influenzae UZA 28 Clinical isolate Streptococcus pneumoniae UZA 29 Clinical isolate Streptococcus pyogenes UZA 30 Clinical isolate Streptococcus viridans UZA 31 Clinical isolate Staphylococcus aureus UZA 32 Clinical isolate Klebsiella pneumoniae UZA 33 Clinical isolate Escherichia coli UZA 34 Clinical isolate Neisseria meningitidis UZA 35 Clinical isolate Pseudomonas aeruginosa UZA a ITG: Instituut voor Tropische Geneeskunde, Antwerp, Belgium, b ATCC: American ACCEPTED Type Culture Collection, Manassas, Va, USA, c NCTC: National Collection of Type Cultures, Central Public Health Laboratory, London, UK, d UZA: Universitair Ziekenhuis Antwerpen, Edegem, Belgium, e SEH: Sint Elisabeth Hospital, Tilburg, The Netherlands. 18

485 Table 2a. Overview of test results for all M. pneumoniae positive patients Patient Nr Specimen PCR Real-time mono NASBA Real-time MX NASBA 19 U1A result b IgM IgG c culture 1 BAL + + + 9.61 NA NA ND 2 BA + + + 11.00 NA NA ND 3 NPA + + + 9.76 NA NA ND 4 NPA + + + 14.30 NA NA ND 5 Sputum + + + 11.27 NA NA ND 6 NPA + + + 9.00 NA NA ND NPA + + + 10.55 NA NA ND 7 BAL + + + 11.46 NA NA ND 8 BAL + + - 10.27 NA NA ND 9 TS + + + 7.28 + + - Sputum + + + 9.78 + 10 BAL + + + 14.58 NA NA ND 11 BAL + + + 15.94 NA NA ND 12 NPA + + + 11.50 NA NA ND 13 NPA + + + 11.78 NA NA ND 14 TS + + + 9.89 + + - Sputum + + + 12.68 + 15 a NPA + + + 11.86 - ND - 16 TS + + + 12.30 - + - 17 TS + + - 4.07 + + - Gargle + + - 3.04 + 18 Sputum 1 + + + 13.44 NA NA ND Sputum 2 - + + 7.83 ND 19 Sputum + + + 13.53 + + + TS + - - 1.34 - 20 Gargle + + + 8.69 NA NA - TS + - - 1.56 - 21 a TS + + - 3.46 - ND - Gargle - + - 6.24 - 22 a TS + - - 11.50 - ND - Sputum - + + 13.25 - 23 a Sputum + - - 1.34 + ND - TS - + - 8.29 - 24 Sputum + - - 1.72 - - + TS - - - 4.58 - 25 TS + - - 6.50 + - - Sput - - - 9.71 ND 26 TS + - - 5.58 + - - Sputum + - - 12.52 - 27 Sputum + - - 7.82 - - - TS - - - 11.31 ND 28 a TS + - - 10.02 - ND - Gargle - - - 6.16 ND 29 TS + - - 1.38 - - - Sputum - - - 11.09 ND 30 a Sputum + - - 11.59 - ND - TS - - - 9.99 ND 31 Sputum + - - 13.50 NA NA - TS - - - 8.04 ND 32 Sputum + - - 1.58 NA NA - 33 a TS + - - 1.67 - ND - a : only an acute serum sample available, b : U1A positive above 2.00, c : Seroconversion or significant rise in titre ACCEPTED BA: bronchus aspirate; BAL: bronchoalveolar lavage; NA: not available, ND: not done, NPA: nasopharyngeal aspirate; TS: throat swab

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