Development of Real-Time Multiplex NASBA® for the - Journal of ...

490 Table 2b: Overview

490 Table 2b: Overview of test results for all Legionella positive specimens Nr Specimen PCR Cult. Real-time Real-time uAG IgM IgG mono MX NASBA NASBA a b : only an acute serum sample available, : Seroconversion or significant rise in titre, NA: not applicable, b 1 Lung + + + + + ND ND 2 Lung + + + + + ND ND 3 Lung + + + + ND ND ND 4 Lung + + + + ND ND ND 5 Lung + + + + ND ND ND 6 Sputum + + + - + + ND a 7 Sputum + + + - + + + 8 Sputum + + + - + + - 9 Sputum + - + - + - ND a 10 Water + - + + NA NA NA 11 Water + - + + NA NA NA 12 Water + - + + NA NA NA 13 Water + - + + NA NA NA 14 Water + - + + NA NA NA ND: not done ACCEPTED 20

495 500 Table 3A. Sensitivity of real-time mono and multiplex NASBA assays when tested with a single target: M. pneumoniae Negative control 5 a 50 a 500 a 5000 a LY mono 0/4 4/4 4/4 4/4 ND LY multiplex 0/4 2/4 4/4 4/4 ND TS mono 0/4 2/4 3/4 4/4 ND TS multiplex 0/4 2/4 3/4 4/4 ND BAL mono 0/4 2/4 3/4 4/4 ND BAL multiplex 0/4 2/4 2/4 4/4 ND NA mono 0/4 2/4 3/4 4/4 ND NA multiplex 0/4 2/4 3/4 4/4 ND BA mono 0/4 ND 1/4 2/4 4/4 BA multiplex 0/4 ND 1/4 2/4 4/4 SP mono 0/4 ND 4/6 4/6 4/6 SP multiplex 0/4 ND 3/6 4/6 5/6 Total mono 0/24 10/16 18/26 22/26 8/10 Total multiplex 0/24 8/16 16/26 22/26 9/10 BAL: bronchoalveolar lavage, BA: bronchus aspirate, CCU: colour changing units in the extraction, LY: lysis buffer, NA: nasopharyngeal aspirate, ND: not done, SP: sputum, TS: throat swab a number of CCU in nucleic acid extraction Underlined numbers represent differences in number of samples with correct results between real-time mono and multiplex NASBA. ACCEPTED 21