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Development of Real-Time Multiplex NASBA® for the - Journal of ...

Development of Real-Time Multiplex NASBA® for the - Journal of ...

505 Table 3B.

505 Table 3B. Sensitivity of real-time mono and multiplex NASBA assays when tested with a single target: C. pneumoniae Negative control 0.1 a 1 a 10 a 100 a LY mono 0/4 4/4 4/4 4/4 4/4 LY multiplex 0/4 3/4 4/4 4/4 4/4 TS mono 0/4 4/4 4/4 4/4 4/4 TS multiplex 0/4 4/4 4/4 4/4 4/4 BAL mono 0/4 4/4 4/4 4/4 4/4 BAL multiplex 0/4 3/4 4/4 4/4 4/4 NA mono 0/4 4/4 4/4 4/4 4/4 NA multiplex 0/4 3/4 4/4 4/4 4/4 BA mono 0/4 0/4 0/4 0/4 0/4 BA multiplex 0/4 0/4 0/4 0/4 0/4 SP mono 0/4 0/4 0/4 4/4 4/4 SP multiplex 0/4 0/4 0/4 4/4 4/4 Total mono 0/24 16/24 16/24 20/24 20/24 Total multiplex 0/24 13/24 16/24 20/24 20/24 BAL: bronchoalveolar lavage, BA: bronchus aspirate, IFU: inclusion forming units in the extraction, LY: lysis buffer, NA: nasopharyngeal aspirate, ND: not done, SP: sputum, TS: throat swab a number of IFU in nucleic acid extraction Underlined numbers represent differences in number of samples with correct results between real-time mono and multiplex NASBA. ACCEPTED 22

510 515 Table 3C. Sensitivity of real-time mono and multiplex NASBA assays when tested with a single target: L. pneumophila Negative control 0.1 a 1 a 10 a 100 a 1000 a 10000 a LY mono 0/4 4/4 4/4 4/4 4/4 4/4 ND LY multiplex 0/4 2/4 4/4 4/4 4/4 4/4 ND TS mono 0/4 0/4 2/4 1/4 3/4 4/4 ND TS multiplex 0/4 0/4 1/4 3/4 4/4 4/4 ND BAL mono 0/4 0/4 1/4 3/4 3/4 4/4 ND BAL multiplex 0/4 0/4 0/4 1/4 4/4 4/4 ND NA mono 0/4 0/4 3/4 3/4 2/4 4/4 ND NA multiplex 0/4 0/4 0/4 1/4 2/4 4/4 ND BA mono 0/8 ND 0/8 3/8 4/8 6/8 8/8 BA multiplex 0/8 ND 0/8 1/8 3/8 5/8 6/8 SP mono 0/8 ND 1/8 2/8 4/8 4/8 7/8 SP multiplex 0/8 ND 0/8 0/8 3/8 4/8 7/8 Total mono 0/24 4/16 11/32 16/32 20/32 26/32 15/16 Total multiplex 0/24 2/16 5/32 10/32 20/32 25/32 13/16 BAL: bronchoalveolar lavage, BA: bronchus aspirate, CFU: colony forming units in the extraction, LY: lysis buffer, NA: nasopharyngeal aspirate, ND: not done, SP: sputum, TS: throat swab a number of CFU in nucleic acid extraction Underlined numbers represent differences in number of samples with correct results between real-time mono and multiplex NASBA. ACCEPTED 23

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