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Development of Real-Time Multiplex NASBA® for the - Journal of ...

Development of Real-Time Multiplex NASBA® for the - Journal of ...

520 525 Table 4a:

520 525 Table 4a: Sensitivity of the real-time multiplex NASBA in comparison with the mono real-time NASBA assays in the amplification reaction. Number of in vitro generated RNA molecules in the 24 amplification (n=15-20) 10 50 100 1000 10000 L. pneumophila mono 31% 70% 91% 100% 100% L. pneumophila MX 23% 57% 82% 100% 100% M. pneumoniae mono 33% 82% 89% 100% 100% M. pneumoniae MX 7% 50% 79% 100% 100% C. pneumoniae mono 80% 100% 100% 100% 100% C. pneumoniae MX 65% 100% 100% 100% 100% MX: multiplex Table 4b: Sensitivity of the real-time multiplex NASBA in comparison with the mono real-time NASBA assays in the extraction. ACCEPTED Number of in vitro generated RNA molecules in the extraction (n=10-12) 500 1000 5000 10000 50000 L. pneumophila mono 80% 50% 100% 100% 100% L. pneumophila MX 60% 30% 70% 91% 100% M. pneumoniae mono 20% 40% 80% 91% 100% M. pneumoniae MX 20% 37% 60% 72% 100% C. pneumoniae mono 100% 100% 100% 100% 100% C. pneumoniae MX 30% 45% 100% 100% 100% MX: multiplex

530 Table 5. Sensitivity of real-time multiplex NASBA assays when tested with multiple target in vitro generated RNA molecules. Nr of in vitro generated RNA molecules in the extraction Detection result positive by real-time multiplex NASBA for: M. pneumoniae C. pneumoniae L. pneumophila 33 Lpn + 33 Mpn + 33 Cpn 1/2 2/2 1/2 330 Lpn + 330 Mpn + 330 Cpn 2/2 2/2 2/2 3300 Lpn + 3300 Mpn + 3300 Cpn 2/2 2/2 2/2 330 Lpn + 3300 Mpn + 3300 Cpn 2/2 2/2 0/2 3300 Lpn + 330 Mpn + 3300 Cpn 2/2 2/2 2/2 3300 Lpn + 3300 Mpn + 330 Cpn 2/2 2/2 2/2 Lpn: Legionella pneumophila in vitro generated RNA Mpn: Mycoplasma pneumoniae in vitro generated RNA Cpn: Chlamydia pneumoniae in vitro generated RNA ACCEPTED 25

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