Analysis and characterization of differential gene expression during ...
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Reproductive Biology and Endocrinology BioMed Central Research Analysis and characterization of differential gene expression during rapid trophoblastic elongation in the pig using suppression subtractive hybridization Jason W Ross1 , Morgan D Ashworth1 , AmyGHurst2 , Jerry R Malayer2 and Rodney D Geisert* 1,3 Open Access Address: 1Department of Animal Science, Oklahoma Agriculture Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74078, USA, 2Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078, USA and 3Department of Animal Science, Animal Science Building, Rm 114, Oklahoma State University, Stillwater, OK 75078-6051, USA Email: Jason W Ross - email@example.com; Morgan D Ashworth - firstname.lastname@example.org; Amy G Hurst - email@example.com; Jerry R Malayer - firstname.lastname@example.org; Rodney D Geisert* - email@example.com * Corresponding author Published: 14 February 2003 Reproductive Biology and Endocrinology 2003, 1:23 This article is available from: http://www.RBEj.com/content/1/1/23 Abstract During late peri-implantation development, porcine conceptuses undergo a rapid (2–3 hrs) morphological transformation from a 10 mm sphere to a thin filamentous form greater than 150 mm in length. Elongation of the conceptus is important for establishing adequate placental surface area needed for embryo and fetal survival throughout gestation. Genes involved with triggering this unique transition in conceptus development are not well defined. Objective of the present study was to utilize suppression subtractive hybridization (SSH) to characterize the change in gene expression during conceptus transformation from spherical (8–9 mm) to tubular (15–40 mm) to early filamentous (>150 mm) morphology. Spherical, tubular, and filamentous conceptuses were collected from pregnant gilts and subjected to SSH. Forward and reverse subtractions were performed to identify candidate genes differentially expressed during spherical to tubular and tubular to filamentous transition. A total of 384 transcripts were differentially screened to ensure unique expression. Of the transcripts screened, sequences were obtained for 142 that were confirmed to be differentially expressed among the various morphologies. Gene expression profiles during rapid trophoblastic elongation were generated for selected mRNAs using quantitative realtime PCR. During the transition from tubular to early filamentous conceptuses, sadenosylhomocysteine hydrolase and heat shock cognate 70 kDa expression were significantly enhanced. A novel unknown gene was isolated and shown to be significantly up-regulated at the onset of rapid trophoblastic elongation and further enhanced in filamentous conceptuses. Background As in the majority of mammalian species, successful embryonic development in the pig requires temporally and spatially specific gene expression essential to placental and embryonic differentiation during early gestation. Expressing the appropriate transcripts during development Received: 22 January 2003 Accepted: 14 February 2003 © 2003 Ross et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. of the pig conceptus is essential for expansion of the trophoblast and placental attachment to the uterine surface. Prenatal mortality in the pig ranges from 20% to 46% by term , the majority of which occurs during peri-implantation conceptus development . The peri-implantation period is the most critical stage of conceptus development Page 1 of 12 (page number not for citation purposes)
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