- Page 1 and 2: Molecular Characterization and Gene
- Page 3 and 4: Declaration I hereby do declare tha
- Page 5 and 6: Acknowledgements This work was carr
- Page 7: My Seniors, Dr. Annies Joseph, Dr.
- Page 11 and 12: in response to WSSV challenge 252-2
- Page 13 and 14: α-2M Alpha-2-Macroglobulin ALF Ant
- Page 15 and 16: ppA Prophenoloxidase-Activating Enz
- Page 17 and 18: 1.1 Introduction Chapter 1 Aquacult
- Page 19 and 20: Chapter 1 the giant tiger shrimp (P
- Page 21 and 22: Common names: Chapter 1 Giant tiger
- Page 23 and 24: 1.3.1. Diseases Chapter 1 Diseases
- Page 25 and 26: Lymphoidal parvo-like virus (LPV) B
- Page 27 and 28: Chapter 1 and protective occlusion
- Page 29 and 30: Chapter 1 small brown masses in the
- Page 31 and 32: 1.4.6. Natural epidemics Chapter 1
- Page 33 and 34: Chapter 1 can differentiate WSSV-in
- Page 35 and 36: 1.5.1.1. Probiotics Chapter 1 Alter
- Page 37 and 38: Chapter 1 tried in P. monodon, resu
- Page 39 and 40: Chapter 1 The first and essential i
- Page 41 and 42: Chapter 1 1998). The innate immunit
- Page 43 and 44: 1.6.1 Haemocytes Chapter 1 The haem
- Page 45 and 46: Chapter 1 In crustaceans, the sheet
- Page 47 and 48: Chapter 1 Theopold et al., 2002). C
- Page 49 and 50: Chapter 1 kill/remove the invading
- Page 51 and 52: Chapter 1 forming complexes with a
- Page 53 and 54: 1.7 Antimicrobial Peptides (AMPs) 1
- Page 55 and 56: Chapter 1 the response is very fast
- Page 57 and 58: Chapter 1 Hirsch, 1947). While they
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Chapter 1 AMPs to various degrees u
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1.7.5 Biological activity Chapter 1
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Chapter 1 including the microbes as
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Tachyplesin I Chapter 1 Crustacea P
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Anionic and cationic peptides that
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Anionic and Cationic AMPs with cyst
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Chapter 1 Fig. 1.7. Structural clas
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Chapter 1 Group III: Linear peptide
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Chapter 1 through regular processes
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Chapter 1 ultimately signal to tran
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Chapter 1 divalent polyanionic cati
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Chapter 1 type of transmembrane por
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Chapter 1 other intracellular targe
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Chapter 1 Apidaecin, a short, proli
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Chapter 1 Hultmark, 1987; Gennaro a
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Chapter 1 quite opposite characteri
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Chapter 1 depletion of mitochondria
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Chapter 1 conformations adopted by
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Chapter 1 receptor may be a potenti
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Chapter 1 exposure to their targets
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Chapter 1 generating a rich spectru
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Chapter 1 by enkelytin (Goumon et a
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Lactoferricin Cow Mastitis control
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Chapter 1 trial, in which peptide T
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Chapter 1 a too limited spectrum to
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Chapter 1 number of research groups
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CHAPTER-2 Molecular Characterizatio
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Chapter 2 interaction with negative
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Chapter 2 inhibit the endotoxin or
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Chapter 2 structure be modified to
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Chapter 2 and the region might be l
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Chapter 2 been made of the spectra
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Chapter 2 their discovery and initi
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Chapter 2 terminal pyroglutamic aci
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Chapter 2 One can assume that the p
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Chapter 2 by degranulation into the
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2.2. Materials and Methods 2.2.1. E
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Chapter 2 washed by adding 1 ml 75
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Chapter 2 entry of recombinant DNA
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Chapter 2 and the deduced amino aci
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Chapter 2 Fenneropenaeus, Litopenae
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2.3.1.3. Crustin-1 (GQ334395) Chapt
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2.3.1.3.5. Phylogenetic analysis of
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Chapter 2 sequence also showed high
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Chapter 2 chinensis and F. indicus
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Chapter 2 monodon and Group III of
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Chapter 2 Tiger shrimp (P. monodon)
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Chapter 2 ALF-1 was a partial seque
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Chapter 2 As mentioned before, the
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Chapter 2 Munoz et al., 2004). The
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Table 2.1. Primers used for the stu
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Table 2.4. BLASTn analysis of ALF-2
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Table 2.8. BLASTn analysis of Crust
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Table 2.12. BLASTn analysis of Pena
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Fig.2.5. Agarose gel electrophoreto
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Chapter 2 Fig.2.9. Multiple alignme
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Chapter 2 Fig. 2.12 A bootstrapped
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Chapter 2 Fig. 2.15. Multiple align
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Chapter 2 Fig.2.18. A bootstrapped
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Chapter 2 Fig.2.20. Signal peptide
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Chapter 2 Fig.2.24 Multiple alignme
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Chapter 2 LITOPENAEUS SP. FARFANTEP
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Chapter 2 Fig. 2.28. Nucleotide and
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Chapter 2 Fig.2.33 Multiple alignme
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Chapter 2 LITOPENAEUS SP. FENNEROPE
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Chapter 2 Fig. 2.37. Nucleotide and
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Chapter 2 Fig.2.41 Multiple alignme
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Chapter 2 Fig.2.43 A bootstrapped n
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Chapter 2 Fig. 2.45. Nucleotide and
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Chapter 2 LITOPENAEUS SP. FENNEROPE
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Chapter 2 Fig.2.51 Signal peptide a
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Chapter 2 FENNEROPENAEUS SP. FARFAN
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CHAPTER-3 Molecular Characterizatio
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Chapter 3 certainly help us to unra
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Chapter 3 3.3.1.1. Anti-lipopolysac
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Chapter 3 was predicted out to be 1
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Chapter 3 random coiled structure t
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3.3.1.3.5. Phylogenetic analysis of
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Chapter 3 case has been reported in
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Chapter 3 Multiple alignments were
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Chapter 3 negative bacteria. In shr
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Chapter 3 indicated a random coiled
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Chapter 3 horseshoe crab big defens
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Table 3.4. BLASTn analysis of ALF-2
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Table.3.8. BLASTn analysis of Fi-pe
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Chapter 3 Fig.3.2. Nucleotide and a
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Chapter 3 Fig.3.5. Multiple alignme
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Chapter 3 Fig.3.8. Nucleotide and a
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Chapter 3 Fig.3.12. Multiple alignm
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Chapter 3 Fig.3.14. A bootstrapped
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Chapter 3 Fig.3.17. Signal peptide
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Chapter 3 Fig.3.21. Multiple alignm
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SHRIMPS KIND CRAB LOBSTERS CRABS Ch
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Chapter 3 Fig.3.25. Signal peptide
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Chapter 3 Fig. 3.28. Multiple align
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PENAEIDIN-2 Chapter 3 PENAEIDIN- 3
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Fig.3.31. Nucleotide and amino acid
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Chapter 3 Fig.3.35. Multiple alignm
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Chapter 3 Fig.3.37 Nucleotide and a
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4.1. Introduction Chapter 4 The aqu
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Chapter 4 appearance and have major
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Chapter 4 the tissues has recently
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Chapter 4 with the ability of shrim
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Chapter 4 with a low dose of WSSV b
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Chapter 4 molecules they are likely
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Chapter 4 for understanding gene ex
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Chapter 4 4.3.2. Expression profile
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Expression profile of endonuclease
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Chapter 4 bacterial and fungal infe
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Chapter 4 measuring TSV and YHV loa
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Chapter 4 Of particular interest he
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Chapter 4 large distribution and ab
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Chapter 4 WSSV genes viz. endonucle
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Table 4.2. Primers used for the stu
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(A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
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(A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
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(A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
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(A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
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(A) (B) B 28 1 2 3 4 5 6 7 8 9 10 F
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(A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
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(A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
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(A) (B) B 28 1 2 3 4 5 6 7 8 9 10 C
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CHAPTER-5 Expression Profile of Ant
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Chapter 5 1994). The emergence of b
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Chapter 5 (Bovill et al., 2001). In
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5.2.2. Test diets Chapter 5 Four ex
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5.2.2.4. Preparation of Glucan inco
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Chapter 5 genes (latency related ge
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Chapter 5 On WSSV challenge, crusti
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Chapter 5 5.3.4. Expression profile
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5.3.4.5. Expression profile of pena
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Chapter 5 must firstly rest on a so
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Chapter 5 For the present study two
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Chapter 5 ameobocytes of two marine
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Chapter 5 variation in expression o
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Chapter 5 It has been previously sh
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Chapter 5 manifested in terms of gr
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(A) (B) (C) C CHY CSY CHG CSG C CHY
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(A) (B) (C) C CHY CSY CHG CSG C CHY
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(A) (B) (C) C CHY CSY CHG CSG C CHY
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(A) (B) (C) C CHY CSY CHG CSG C CHY
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(A) (B) -VE CTRL +VE CTRL CHG CSG C
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(A) (B) -VE CTRL +VE CTRL CHG CSG C
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(A) (B) -VE CTRL +VE CTRL CHG CSG C
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(A) (B) -VE CTRL +VE CTRL CHG CSG C
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(A) PRE-CHALLENGE (B) POST-CHALLENG
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(A) PRE-CHALLENGE (B) (C) (D) CONTR
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(C) (D) (A) PRE-CHALLENGE (B) CONTR
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(A) (B) G M HP HR I Chapter 5 Fig.
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Chapter 5 Fig. 5.26. Post challenge
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6.1. Introduction Chapter 6 Prophyl
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Chapter 6 production of inhibitory
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6.2. Materials and methods 6.2.1. E
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Chapter 6 tissue cDNA was performed
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Chapter 6 gene was supported by Bac
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Chapter 6 treated group of shrimps.
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Chapter 6 the gene was found to be
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Chapter 6 found to support minimum
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Chapter 6 is induced upon bacterial
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Chapter 6 all the target genes, exc
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Chapter 6 Detailed tissue-wise anal
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Chapter 6 Gills and intestine was f
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(A) (B) Fig. 6.1. Experimental diet
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(A) (B) (C) Fig. 6.3. Expression pr
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(A) (B) (C) C B M BM C B M BM Fig.
- Page 389 and 390:
(A) (B) (C) Fig. 6.7. Expression pr
- Page 391 and 392:
(A) (B) (C) Chapter 6 Fig. 6.9. Exp
- Page 393 and 394:
(A) (B) -VE CTRL +VE CTRL B M BM Ch
- Page 395 and 396:
(A) (B) -VE CTRL +VE CTRL B M BM Ch
- Page 397 and 398:
(A) (B) -VE CTRL +VE CTRL B M BM Ch
- Page 399 and 400:
(A) (B) -VE CTRL +VE CTRL B M BM Ch
- Page 401 and 402:
(A) PRE-CHALLENGE (B) POST-CHALLENG
- Page 403 and 404:
(A) PRE-CHALLENGE (B) POST-CHALLENG
- Page 405 and 406:
(A) (C) (D) PRE-CHALLENGE POST-CHAL
- Page 407 and 408:
(A) (B) G M HP HR I Chapter 6 Fig.
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Chapter 6 Fig. 6.27. Post challenge
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7.1. Summary Chapter 7 Tropical shr
- Page 413 and 414:
Salient findings Chapter 7 � From
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Chapter 7 � Tissue-wise expressio
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Chapter 7 application in shrimp cul
- Page 419 and 420:
References Aboudy, Y., Mendelson, E
- Page 421 and 422:
References Anggraeni, M.S., Owens,
- Page 423 and 424:
References Bals, R., Wang, X., Zasl
- Page 425 and 426:
References derived from the N-termi
- Page 427 and 428:
References Breukink, E., de Kruijff
- Page 429 and 430:
References Burgents, J.E., Burnett,
- Page 431 and 432:
References 1,3-β-D-glucan-binding
- Page 433 and 434:
References Chen, J.Y., Chuang, H.,
- Page 435 and 436:
References Cole, A.M., Hong, T., Bo
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References Dathe, M., Wieprecht, T.
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References Diamond, G., Zasloff, M.
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References Faber, C., Stallmann, H.
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References Flint, S.J., Enquist, L.
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References Gennaro, R., Zanetti, M.
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References Gudmundsson, G.H., Lidho
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References Harwig, S.S., Swiderek,
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References Hirakura, Y., Kobayashi,
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References Huang, C.C., Song, Y.L.
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References Itami, T., Takahashi, Y.
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References Jiravanichpaisal, P. Whi
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References Kang, J.H., Shin, S.Y.,
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References Kono, T., Savan, R., Sak
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References rich peptide derived fro
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References Li, L., Wang, J.X., Zhao
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References Liu, C.H., Cheng, W., Ku
- Page 469 and 470:
References Lorenzon, S., de Guarrin
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References Marone, M., Mozzetti, S.
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References Medvinsky, A., Dzierzak,
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References Moriarty, D.J.W. 1996. M
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References antimicrobial peptide fr
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References Otta, S.K., Karunasagar,
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References simplex virus has heptad
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References infected shrimp. Thesis
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References Litopenaeus vannamei cha
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References Roth, B.L., Poot, M., Yu
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References Sathish, S., Musthaq, S.
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References a proline-arginine-rich
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References Smith, V.J., Fernandes,
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References Song, Y.L., Cheng, W., W
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References experimental system. In:
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References the black tiger shrimp P
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References Tapay, L.M., Cesar, E.,
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References Touhy, K.M., Probert, H.
- Page 505 and 506:
References van Hulten, M.C., Reijns
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References Vives, R.R., Imberty, A.
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References Wang, Y.C., Chang, P.S.,
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References Williams, M.J., Rodrigue
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References Wu, W., Zong, R., Xu, J.
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References Yodmuang, S., Tirasophon
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References Zhan, W.B., Wang, Y.H. 1
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Penaeus monodon anti-lipopolysaccha
- Page 521 and 522:
Penaeus monodon crustin-like antimi
- Page 523 and 524:
Penaeus monodon crustin-like antimi
- Page 525 and 526:
Penaeus monodon penaeidin-like anti
- Page 527 and 528:
Penaeus monodon elongation factor m
- Page 529 and 530:
Fenneropenaeus indicus anti-lipopol
- Page 531 and 532:
Fenneropenaeus indicus penaeidin-li
- Page 533 and 534:
Fenneropenaeus indicus beta-actin m
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PUBLICATIONS
- Page 537 and 538:
Short sequence report Molecular cha
- Page 539 and 540:
Fig. 2. Multiple alignment of nucle
- Page 541 and 542:
220 The phylogenetic relationships
- Page 543 and 544:
Table 1 Rearing conditions and wate
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5 ′ cttgtggttgacaatggctccg3
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Expression of WSSV genes in the hae
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Fig. 4. Expression profile of crust
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S.P. Antony et al. / Immunobiology