Novocastra™ Lyophilized Mouse Monoclonal Antibody Cytokeratin ...

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NCL-CK34BE12 Page 1
Novocastra Lyophilized Mouse Monoclonal Antibody Cytokeratin (1/5/10/14) Product Code: NCL-CK34BE12 Intended Use For in vitro diagnostic use. NCL-CK34BE12 is intended for the qualitative identification by light microscopy of Cytokeratin (1/5/10/14) molecules in paraffin sections. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist. Principle of Procedure Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen. Clone 34βE12 Immunogen Solubilised keratin extracted from human stratum corneum. Specificity Human cytokeratins 1, 5, 10 and 14 with molecular weights of 68, 58, 56.5 and 50 kD respectively. Reagent Composition NCL-CK34BE12 is a lyophilized tissue culture supernatant in phosphate-buffered saline (pH 7.6) with 1% bovine serum albumin carrier protein and containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label. Ig Class IgG1, kappa Total Protein Concentration Total Protein Refer to vial label for lot specific total protein concentration. Antibody Concentration Greater than or equal to 11.7 mg/L as determined by ELISA. Refer to vial label for lot specific Ig concentration. Recommendations On Use Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra Epitope Retrieval Solution pH 6. Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Visualization: Please follow the instructions for use in the Novolink Polymer Detection Systems. For further product information or support, contact your local distributor or regional office of Leica Microsystems, or alternatively, visit the Leica Microsystems’ Web site, www.leica-microsystems.com Storage and Stability Store unopened antibody at 2–8 °C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. Do not use after expiration date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 2–8 °C. For long term storage, it is recommended that aliquots of the reconstituted antibody are stored frozen at -20 °C (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use. Return to 2–8 °C immediately after use. Storage conditions other than those specified above must be verified by the user. Specimen Preparation The recommended fixative is 10% neutral-buffered formalin for paraffin-embedded tissue sections. Warnings and Precautions This reagent has been prepared from the supernatant of cell culture. As it is a biological product, reasonable care should be taken when handling it. This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or available from www.leica-microsystems.com Consult federal, state or local regulations for disposal of any potentially toxic components. Specimens, before and after fixation, and all materials exposed to them, should be handled as if capable of transmitting infection and disposed of with proper precautions. 1 Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek medical advice. NCL-CK34BE12 Page 2
Page 1: Novocastra Lyophilized Mouse Monoc
Page 5 and 6: Bibliography - General 1. National
Page 7 and 8: Mises en Garde et Précautions Ce r
Page 9 and 10: Novocastra Anticorpo Monoclonale M
Page 11 and 12: Limitazioni Generali L’immunoisto
Page 13 and 14: Dieses Reagenz enthält Natriumazid
Page 15 and 16: Novocastra Anticuerpos Monoclonal
Page 17 and 18: NCL-CK34BE12 está recomendado para
Page 19 and 20: Avisos E Precauções Este reagente
Page 21 and 22: Novocastra Frystorkad Monoklonal M
Page 23 and 24: Den kliniska tolkningen av all fär
Page 25 and 26: Ο χειρισμός δειγμά
Page 27 and 28: Novocastra Lyophiliseret Monoklona
Page 29 and 30: Antistoffer fra Leica Biosystems Ne
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