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prueba de histamina para atun

Veratox Procedure for Histamine Note: Samples must be diluted before performing test. Please read kit instructions completely before beginning. Questions? Call 800/234-5333 or 517/372-9200. 1. Add 100 µL conjugate to each red marked mixing well. 2. Add 100 µL of controls and diluted samples to their respective wells. 3. Mix. Transfer 100 µL to antibody wells. Incubate for 10 minutes. 4. Dump liquid from antibody wells. 5. Wash wells thoroughly 4 times with diluted wash buffer. 6. Carefully tap out remaining liquid on absorbent paper towel. 7. Transfer 100 µL substrate from reagent boat to antibody wells using 12-channel pipettor. Incubate for 10 minutes. 8. Transfer 100 µL Red Stop from reagent boat to antibody wells. Product Specifications 9. Read results using a microwell reader with a 650 nm filter. Lower limit of detection: 2 ppm Range of quantitation: 2.5 ppm - 50 ppm Controls provided: 0, 2.5, 5, 10, 20 and 50 ppm Testing time: 20 minutes Antibody cross-reactivity: Specific for histamine 620 Lesher Place • Lansing, MI 48912 800/234-5333 (USA and Canada only) • 517/372-9200 • fax: 517/372-2006 e-mail: foodsafety@neogen.com • www.neogen.com ©Neogen Corporation 2007. Neogen and Veratox are registered trademarks of Neogen Corporation, Lansing, MI. V-Hist-1007

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