13.07.2015 Views

Marie-Christine LE PASLIER - Inra

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Génotypage SNPs: comparatifTaqMan®Golden Gate®Infinium®Taqman® Golden Gate® Infinium®BeadXpress/VeraCodeBeadStationméthode dediscriminationalléliqueASO hybridation5’ exonuclease activityof PCR-DNApolASO/LSO hybridationExtension ASO primerLSO ligationPCRASO hybridationExtension ASO primermultiplexage Non 48 / 96 / 144 / 384 1536 / 384 3000– 90 000nombre d’individus contraintes dudesign des sondes coût / SNP / ind coût initial (K€) (0,5) (80) (80-250)débitN x 3841 jour4 x 964 jours12 x 964 joursdonnées générées 384/384147 000/96588 000/ 4 jours4 800 000/9657 600 000/ 4 joursEPGV Unité 1279_GAP5

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