MALDI-TOF-MS Cooking Book
MALDI-TOF-MS Cooking Book MALDI-TOF-MS Cooking Book
MALDI-TOF-MS Cooking Book Ver. 1.0 Prepared by T.Nirasawa 2-May-03
- Page 2 and 3: Table matrix mono MW for size (g)
- Page 4 and 5: Double Layer Preparation CCA in ace
- Page 6 and 7: Double Layer Preparation SA in EtOH
- Page 8 and 9: DHB in TA (10mg/ml) 4l (10pmol/l)
- Page 10 and 11: 2,4,6-THAP in 50ACN (10mg/ml) 4l (
- Page 12 and 13: AnchorChip STD ZipTip CCA stock
- Page 14 and 15: DNA RNA oligo 3HPA(10 mg/ml)+ Diamm
- Page 16 and 17: Double stranded DNA 6-aza-2-thiothy
- Page 18 and 19: Polymer matrix in THF(10mg/ml) 8l
<strong>MALDI</strong>-<strong>TOF</strong>-<strong>MS</strong><br />
<strong>Cooking</strong> <strong>Book</strong><br />
Ver. 1.0<br />
Prepared by T.Nirasawa<br />
2-May-03
Table <br />
matrix mono MW for size (g) Parts No. (Daltonics)<br />
-cyano-4-hydroxy-cinnamicacid<br />
<br />
189.17 # peptide<br />
(CCA)<br />
<br />
sinapinic acid (SA) 224.22<br />
2,5-dihydroxy-benzoic acid<br />
(2,5-DHB)<br />
Super DHB<br />
(SDHB)<br />
3-Hydroxypicolinic acid<br />
(3-HPA)<br />
α-Cyano-4-hydroxycinnamic acid<br />
methyl ester<br />
(CNME)<br />
2,3,4 -Trihydroxyacetophenone<br />
(2,3,4-THAP)<br />
2,4,6 –Trihydroxyacetophenone<br />
(2,4,6-THAP)<br />
154.12<br />
187.20<br />
# peptide<br />
# protein<br />
# protein<br />
# glycopeptide<br />
# glycoprotein<br />
# oligosaccharide<br />
# polymer<br />
# glycopeptide<br />
# glycoprotein<br />
# oligosaccharid<br />
<br />
<br />
<br />
<br />
<br />
139.11 <br />
<br />
<br />
203.20 <br />
<br />
<br />
168.15 <br />
<br />
168.15<br />
6-aza-2-thiothymine (ATT) 143.02<br />
trans-3-indole-acrylic acid<br />
(IAA)<br />
Dithranol<br />
(DIT)<br />
187.20<br />
226.23<br />
#201345<br />
#203073<br />
#201346<br />
#203074<br />
#209813<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
# polymer<br />
<br />
# aromatic<br />
<br />
compound<br />
# polymer<br />
# aromatic<br />
compound
CCA in TA ( )<br />
4l<br />
(5pmol/l)<br />
1l<br />
mix<br />
1l on target<br />
<br />
#26755 MTP 384 massive target T<br />
#26993 MTP 384 massive target gold plated T <br />
#209519 MTP 384 target ground steel T F <br />
#209520 MTP 384 target plate polished steel T F
Double Layer Preparation<br />
CCA in acetone ( )<br />
CCA in TA ( )<br />
4l<br />
(5pmol/l)<br />
1l<br />
l mix<br />
<br />
1l <br />
<br />
#209520 MTP 384 target plate polished steel T F
SA in TA ( )<br />
4l<br />
(10pmol/l)<br />
1l<br />
mix<br />
1l on target<br />
<br />
#26755 MTP 384 massive target T<br />
#26993 MTP 384 massive target gold plated T <br />
#209519 MTP 384 target ground steel T F <br />
#209520 MTP 384 target plate polished steel T F
Double Layer Preparation<br />
SA in EtOH ( )<br />
SA in TA ( )<br />
4l<br />
(10pmol/l)<br />
1l<br />
l mix<br />
<br />
1l <br />
<br />
#209520 MTP 384 target plate polished steel T F
DHB in TA (10mg/ml)<br />
4l<br />
<br />
(5pmol/l)<br />
1l<br />
mix<br />
1l on target<br />
<br />
#26755 MTP 384 massive target T<br />
#26993 MTP 384 massive target gold plated T <br />
#209519 MTP 384 target ground steel T F
DHB in TA (10mg/ml)<br />
4l<br />
<br />
(10pmol/l)<br />
1l<br />
mix<br />
1l on target<br />
<br />
#26755 MTP 384 massive target T<br />
#26993 MTP 384 massive target gold plated T <br />
#209519 MTP 384 target ground steel T F
SDHB in TA (10mg/ml)<br />
4l<br />
<br />
(10pmol/l)<br />
1l<br />
mix<br />
1l on target<br />
<br />
#26755 MTP 384 massive target T<br />
#26993 MTP 384 massive target gold plated T <br />
#209519 MTP 384 target ground steel T F
2,4,6-THAP in 50ACN<br />
(10mg/ml)<br />
4l<br />
<br />
(10pmol/l)<br />
1l<br />
mix<br />
1l on target<br />
<br />
<br />
<br />
#26755 MTP 384 massive target T<br />
#26993 MTP 384 massive target gold plated T <br />
#209519 MTP 384 target ground steel T F<br />
#209520 MTP 384 target plate polished steel T F
DHB in 30%EtOH (10mg/ml)<br />
4l<br />
(5pmol/l)<br />
1l<br />
mix<br />
1l on target<br />
<br />
#26755 MTP 384 massive target T<br />
#26993 MTP 384 massive target gold plated T <br />
#209519 MTP 384 target ground steel T F
AnchorChip STD<br />
<br />
<br />
<br />
ZipTip<br />
CCA stock <br />
1mg/ml acetone<br />
CCA working( M ) 2:1= EtOH : acetone<br />
( W )<br />
0.1%TFA in H 2 O<br />
<br />
( R )<br />
6:3:1<br />
<br />
EtOH : acetone : 0.1%TFA<br />
0.53l<br />
600m anchor<br />
Matrix ( M ) 1l<br />
0.53l<br />
600m anchor<br />
Matrix ( M ) 1l<br />
<br />
W 4l <br />
<br />
<br />
<br />
R 1l <br />
#209513 MTP AnchorChip 600/384 T F
Thin Layer Affinity AnchorChip Preparation<br />
CCA ( M ) 2l<br />
<br />
600m anchor<br />
<br />
<br />
1<br />
<br />
2<br />
<br />
<br />
CCA stock <br />
97% acetone : 0.1% TFA<br />
CCA working( M ) 1:1<br />
<br />
CCA stock : 97% acetone + 0.1% TFA<br />
( W )<br />
0.1%TFA in H 2 O<br />
<br />
( R )<br />
6:3:1<br />
<br />
EtOH : acetone : 0.1%TFA<br />
<br />
14l<br />
600m anchor<br />
3<br />
W 35l<br />
<br />
<br />
W 35l anchor<br />
<br />
<br />
<br />
<br />
Thin Layer<br />
PSD,LIFT<br />
R 1l<br />
<br />
#209513 MTP AnchorChip 600/384 T F
DNA RNA oligo<br />
3HPA(10 mg/ml)+ Diammonium hydrogen citrate (DAC 1 mg/ml) in H2O<br />
DNA(RNA)<br />
pmol/l<br />
3HPA 1l<br />
400m anchor<br />
NH4+<br />
<br />
<br />
<br />
1l<br />
00m anchor<br />
<br />
MTP AnchorChip 400/384 T F
DNA RNA oligo<br />
2,4,6 –Trihydroxyacetophenone (2,4,6-THP) in 50% ACN (10mg/ml)<br />
2,3,4 -Trihydroxyacetophenone (2,3,4-THP) in 50% ACN (10mg/ml)<br />
Diammonium hydrogen citrate (e.g. Fluka Part No. 09833) in 50% ACN (DAC 1 mg/ml)<br />
THAP 2:1= 2,4,6-THP: 2,3,4-THP+DAC<br />
DNA(RNA)<br />
pmol/l<br />
NH4+<br />
THAP mix<br />
<br />
<br />
1<br />
<br />
<br />
#209520 MTP 384 target plate polished steel T F
Double stranded DNA<br />
6-aza-2-thiothymine (ATT)<br />
DNA(DS)<br />
pmol/l<br />
NH4+<br />
ATT 10mg/ml in 50% ACN<br />
<br />
<br />
1<br />
<br />
<br />
#209520 MTP 384 target plate polished steel T F
Polymer <br />
DHB in TA (10mg/ml)<br />
4l<br />
polymer (20pmol/l)<br />
1l<br />
mix<br />
1l on target<br />
N2<br />
<br />
<br />
Table 2
Polymer <br />
matrix in THF(10mg/ml)<br />
8l<br />
polymer (20pmol/l)<br />
2l<br />
mix<br />
<br />
3l on target<br />
<br />
<br />
<br />
Table 2
Table Polymer<br />
matrix mono MW for size (g) Parts No. (Daltonics) Salt<br />
α-cyano-4-hydroxy-cinnamicacid<br />
1<br />
#201344<br />
189.17 Tween 20<br />
(CCA)<br />
5<br />
#203072<br />
1<br />
#201345<br />
sinapinic acid (SA) 224.22 Polyacrylic acid<br />
5<br />
#203073<br />
2,5-dihydroxy-benzoic acid<br />
PEG<br />
1<br />
#201346<br />
NaCl<br />
154.12<br />
(DHB)<br />
PPG<br />
5<br />
#203074<br />
KCl<br />
2,4,6 –Trihydroxyacetophenone<br />
1<br />
#206154<br />
168.15 Poly(ethylene terephthalate)<br />
(2,4,6-THAP)<br />
5<br />
#206153<br />
PMMA<br />
PS<br />
silver trifluoroacetate<br />
trans-3-indole-acrylic acid<br />
1<br />
#209803<br />
187.20 Poly(butyl acrylate)<br />
silver nitrate<br />
(IAA)<br />
5<br />
#209801<br />
Dendrimer<br />
Silver (I) acetylacetonate<br />
Polybutadiene<br />
Polystyrene<br />
PPG<br />
Dithranol<br />
PMMA<br />
1<br />
#209783<br />
silver trifluoroacetate<br />
226.23<br />
(DIT)<br />
Poly(methyl acrylate) 5<br />
#209785<br />
silver nitrate<br />
Poly(ethylene terephthalate)<br />
Polycarbonate<br />
Fluka<br />
silver trifluoroacetate<br />
all-trans-Retinoic acid 300.40 PS ( > 60kDa )<br />
95152<br />
silver nitrate<br />
9-nitro-anthracene<br />
Fullerene<br />
Fluka<br />
223.23<br />
(9NA)<br />
Dendrimer<br />
72740<br />
5-Chlorosalicylic acid<br />
Aldrich<br />
172.57 Si oil<br />
(5CSA)<br />
C7,090-8<br />
2’-(4-hydroxyphenylazo)benzoicacid<br />
242.24<br />
Polycarbonate<br />
Nylon<br />
Fluka<br />
54793<br />
(HABA)<br />
Tween 85<br />
1,4-Bis(5-phenyloxazol-2-yl)benzene<br />
Polyisoprene<br />
Fluka<br />
364.40<br />
Silver (I) acetylacetonate<br />
(POPOP)<br />
Polybutadiene<br />
15150<br />
3-amino-quinoline<br />
Fluka<br />
144.18 basic compound<br />
(3AQ)<br />
7336<br />
2,6-Dihydroxyacetophenone<br />
Fluka<br />
152.15<br />
(DHAP)<br />
37468
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