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Analisi dell’equilibrio <strong>di</strong> legame a saturazione(Scatchard plot)•Cellule GM7373 – 180.000 cells/pozzetto in piastra da 48 pozzetti•Aggiunti 100 microlitri <strong>di</strong> terreno/pozzetto contenente• 125 I-FGF2 4 ng/ml, 8 ng/ml, 16 ng/ml, 40 ng/ml, 80 ng/ml, 100 ng/ml• +/- FGF2 300x•Campioni in quintuplicato125 I-FGF2: attività specifica 358 CPM/fmoleI VALORI SONO OTTENUTI DA HOT me<strong>di</strong>o – COLD me<strong>di</strong>o[ 125 I-FGF2] CPM caricate Lavaggio F pH4 BB/F4 ng/ml9936863224.112450.680.0288 ng/ml143061297536.243020.840.02316 ng/ml289952644173.854111.150.015640 ng/ml7838572462202.405631.570.007880 ng/ml154666143609401.1410632.970.0074

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