Egzersiz ÃevrimiÃ§i Dergi - SÃ¼leyman Demirel Ãniversitesi

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Effects Of Football Matches On Lipid Peroxidation And Antioxidant In Blood Of Soccers Nuray Oztasan*, Yücel Ocak**, Ismail Kucukkurt***, Lütfi Akgün*, Abdullah Eryavuz*** * Department of Physiology, Faculty of Medicine, Afyon Kocatepe University, Turkey ** Department of Sports, Faculty of Sports and Training, Afyon Kocatepe University, Turkey *** Department of Physiology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Turkey nurayoztasan@hotmail.com Abstract We investigated effects of soccer play as competion sport on lipid peroxidation and antioxidant levels in blood of young male soccer players.. Seventeen young male volunteers were used in this study. Volunteers have no clinical complaints and problems. They played football at least 4 years. Blood samples were taken from the volunteers before and after soccer. Lipid peroxidation levels as malondialdehyde (MDA), antioxidant activity (AOA), methemoglobin, total cholesterol, tryglycerides total nitric oxide (NO) and glucose levels were measured in the plasma samples. MDA and glucose levels at beginning of the sport were lower (P< 0.05) than in end of the competition although trigliserid level was no significant lower in the end of the competition compared to the begining competition. There were no change in antioksidan activities, NO, urea and methemoglobin levels. In conclusions, these data demonstrate that lipid peroxidation increases in the plasma of soccer without effecting antioxidant activities. Hence, antioxidats supplementation may play a role against oxidative stres in plasma of soccer players Key words: Competition sport, MDA, plasma antioxidant activity, Methemoglobin, nitric oxid
egzersiz 2007 • Sayı 2 • No: 1 20 Introduction For most animals, mobility is essential for survival. An elevated metabolic rate as a result of exercise can dramatically increase oxygen consumption in the locomotive muscles and heart as well as other tissues (Gül et al. 2006). In addition during exercise; the process of delivering the oxygen to working muscles may actually result in damage to polyunsaturated fatty acids in membrane structures. This has been documented by numerous investigations demonstrating increases in by-products of lipid peroxidation following exercise (Chevion et al. 2003). Long-term physical exercise of moderate intensity has been demonstrated to increase the longevity of rats, however, a considerable number of studies have demonstrated that oxidative stress can occur following a bout of exercise (Ramel et al. 2004). A number of potential pathways exist for exercise-related oxidant production. Oxygen consumption can increase several-fold with exercise; (Tsai et al. 2001). During intense exercise, fibers in active muscles may become hypoxic. During ischemia, xanthine is formed via anaerobic metabolism of ATP and xanthine dehydrogenase is converted to xanthine oxidase. During reperfusion, with the resulting increase in oxygen load, xanthine oxidase still converts hypoxanthine to uric acid, but utilizes oxygen as the electron acceptor forming superoxide. Muscle mitochondria undergo increased uncoupling and superoxide generation with increasing temperatures. Therefore exercise-induced hyperthermia may cause oxidative stress (Tonkonogi et al. 2000). Auto-oxidation of oxyhemoglobin to methemoglobin results in the production of superoxide and the rate of formation of methemoglobin can increase with exercise (Deaton et al. 2003). The benefit of exercise in promoting good health and preventing various diseases is well known (Brites et al. 1999). However, chronic exercise also represents a form of oxidative stress to the organisms and therefore can alter the balance between pro-oxidants and antioxidants (Li Li, 1999). Considering that the physical activity and exercise physiology practices are performed to increase performance and capacity (Bloomer et al. 2004). Although there are many studies dealing with some antioxidant enzymes and plasma antioxidant activity (AOA) on blood of sucer [Brites et al., 1999; ], little data is available on the blood lipid parameters and antioxidant levels of soccer players. Aim of the current study was to test the football competetion and its possible beneficial effect on antioxidantive defense system by evaluating lipid profile included triglycerides (TG), total cholesterol (Chol), lipid peroxidation ( as plasma MDA and AOA), nitric oxide (NO), urea and glucose levels in the bood of soccer players. MATERYAL AND METODS Subjects In the present study, seventeen male football players under regular training between the ages of 20 and 22, and within a weight range of 68-72 kg. were used, The subjects were selected from l university physical education students, who gave their written consent prior to participating in this study after design and risk had been described to them. The subjects had no major medical illnesses. They were physically active but not involved in regular high athletic training such as endurance. No subject reported sedative use within the 6 months prior to the study and none was taking antioxidant compounds, including vitamins and medications (anti-inflammatory agents). This study was approved by the Ethical Committee for Research on Human Subjects in Afyon Kocatepe University. (Afyon, Turkey)” and conformed to the guidelines of the Helsinki Conference for research on human subjects. Blood sampling The basal venous blood was drawn from all subjects participating in this study just before the competition and immediately after the competition. The bood samples were collected in regular heparinized vacutainer tubes and then plasma samples were obtained by centrifugation of the blood samples at 1500 g for 10 min. Plasma was used determine plasma, malondialdehyde (MDA), antioxidant activity, methemoglobin, total kolesterol, tryglycerides ve glucose levels.
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