TARTU ÃLIKOOL LOODUS- JA TEHNOLOOGIA TEADUSKOND ...

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Sisukord LÜHENDID ................................................................................................................................ 3 SISSEJUHATUS .........................................................................................................................4 1. KIR<strong>JA</strong>NDUSE ÜLEVAADE .................................................................................................. 5 1.1 Rasvade biokeemia ................................................................................................................................................ 5 1.1.1 Rasvade struktuur ja nomenklatuur .......................................................................... 5 1.1.5 Küllastumata ja polüküllastumata rasvhapped ......................................................... 7 1.1.2 Transrasvad ...............................................................................................................8 1.1.3 Kolesterool ...............................................................................................................9 1.1.4 Kolesteriidid ............................................................................................................11 1.2 Lipiidide metabolism ...................................................................................................... 12 1.2.1 Beeta-oksüdatsioon ................................................................................................. 12 1.2.3 Rasvhapete süntees .........................................................................................................................................13 1.2.4 Rasvade varundamine C. elegansis ...................................................................... 15 2. EKSPERIMENTAALNE OSA ............................................................................................. 17 2.1 Töö eesmärgid ................................................................................................................ 17 2.2 Materjalid ja metoodika ..............................................................................................................................................17 2.2.2 Vanuse sünkroniseerimine .........................................................................................................................................17 2.2.2 Värvimismeetod Oil Red O .................................................................................... 19 2.2.2 Elueakatse ...............................................................................................................22 2.2.3 Katse varurasva hulga hindamiseks ........................................................................ 22 2.3 Tulemused ..............................................................................................................................................23 2.3.1 Eelkatsetused ..........................................................................................................23 2.3.2 Eluea katse .............................................................................................................. 23 2.3.3 Rasvavaru hindamine ............................................................................................. 24 2.4 Arutelu ............................................................................................................................ 26 KOKKUVÕTE ..........................................................................................................................27 KIR<strong>JA</strong>NDUSE LOETELU .......................................................................................................30 LISAD .......................................................................................................................................35 1. Foto Oil Red O meetodil värvitud ussist .......................................................................... 35 2. Eluea katse tulemuste tabel .............................................................................................. 35 Lihtlitsents ................................................................................................................................36 2
LÜHENDID TFA – transrasvhape, trans fatty acid PUFA – polüküllastumata rasvhape, poly-unsaturated fatty acid MUFA – monoküllastumata rasvhape, mono-unsaturated fatty acid chol – kolesterool CE – kolesterüül-ester, cholesteryl ester (ehk kolesteriid) LD – lipiidne tilgake, lipid droplet NGM – nematoodi kasvukeskkond, Nematode Growth Medium LDL – madala tihedusega lipoproteiin, Low Density Lipoprotein HDL – suure tihedusega lipoproteiin High Density Lipoprotein 3
Page 1: TARTU ÜLIKOOL LOODUS- JA TEHNOLOOG
Page 5 and 6: 1. KIRJANDUSE ÜLEVAADE 1.1 Rasvade
Page 7 and 8: 1.1.5 Küllastumata ja polüküllas
Page 9 and 10: nende toimemehanisme molekulaarsel
Page 11: Enamasti vähendab kolesterool kana
Page 14 and 15: katalüsaatoriks atsetüül-CoA ATP
Page 16 and 17: uuritavate haiguste juurde lisandun
Page 18 and 19: Protseduur: Järgneva protseduuri t
Page 20 and 21: 9. Filter 0.2 µM 10. Tris puhverla
Page 22 and 23: 30 mM NaPIPES pH 7.4, 0.2% beta-ME
Page 24 and 25: 2.3.3 Rasvavaru hindamine Värvitud
Page 26 and 27: 2.4 Arutelu Transrasva teistsugused
Page 29 and 30: The effects of cis- and trans isome
Page 31 and 32: D. Falcone, et al. (2004). Regulati
Page 33 and 34: and toxicology 48(1):359 391. L. Ra
Page 35 and 36: LISAD 1. Foto Oil Red O meetodil v

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