FLEISCHWIRTSCHAFT international 1/2017

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................................................ 46 Fleischwirtschaft international 1_2017 Testing Methods Innovation in food control Source: POPOVSKI et al. FLEISCHWIRTSCHAFT international 1_2017 Fig. 5: Electrophoregram of duplex PCR: #1–50 bp ladder #2 and #3–GMO-free soy #4–positive control #5–negative control #6–non-specific amplification #7–chicken sausages without soy #8 and #9–chicken sausage containing soy. ducted to perform aduplex PCR with both pairs of primers, one to amplify the part of the lectin gene, end the second to prove the presence of the insert. So,the single tube contains 2.5 mM MgCl2, 0.4 mM dNTP, 0.2 mM primers (GM03/GM04 and RRS1-F/RRS1- R), 1.0UAmpliTaqGold polymerase and 100ngofsoy DNA. The annealing temperature of 60 °C and the needed concentration of 2.5 mM MgCl2 were used to amplify both fragments at once. This double PCR enables faster detection of GM soy.The method is cheaper and quicker.The results gained through the experiment did not show false positive or false negative values. The verifi cation was done by agarose gel electrophoresis (Fig. 5). The positive control (Fig. 5, #4) and the samples that contained DNA derived from the expression box resulted with two fragments of 118and 74 bp. The negative control and the samples that were not genetically modifi ed resulted with just one fragment of 118bpfrom the lectin gene. The control did not show any fragment; this was aproof for the absence of any contamination and excludes any possibility of false positive results. Practicle importance and application In order to reduce the expenses a successful merging of the procedures for endogene and transgene amplifi cation in asingle tube was realized. Merging these procedures enables faster detection of GM-soy presence. It offers apossibility to eliminate many negative samples before the quantifi cation step with real-time PCR will be taken. The results did not show any false positive or false negative data. They were well-matched with those from the separately accomplished reactions. References 1. DOYLE,J.J. and DOYLE,J.L. (1987): A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phitochem Bull. 19,11–15.–2.HOLST-JENSEN, A., RONNING,S.B., LOVSETH,A.and BERDAL, K.G. (2003): PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal. Bioanal. Chem. 375, 985–993. –3.MEYER, R. and JACCAUD,E.(1997): Detection of geneticallymodified soya in processed food products: development and validation of aPCR assay for the specific detection of Glyphosate-Tolerant Soybeans. Proceedings of the Euro Food Chem IX Conference, Interlaken, Switzerland, Event No. 220, 1, 23–28. – 4. MISKOSKA-MILEVSKA,E., POPOVSKI,T.Z, DIMITRIEVSKA,B.and PORCU,K.(2011): Isolation of DNA for fragment analyses from tomato leaves and seeds. XVI Savetovanje obiotehnologiji. Zbornik radova 16 (18),59–64. –5.MULIS,K.B. and FALOONA,F.A. (1987): Specific synthesis of DNA in vitro via apolymerase catalized chain reaction. Methods in Enzymology 155, 335. –6.OXTOBY,E.and HUGHES,M.A. (1990): Engineering herbicide tolerance into crops. Trends Biotech. 8, 61–65. –7.PÖPPING,B.(2001): Methods for the detection of geneticallymodified organisms: Precision, pitfalls and proficiency.International Laboratory 31 (4), 23–29. –8.WEI,D., LITAO,Y., KAILIN,S., BANGHYUN,K., GIJS,A.K., HANS,J.P.M., et al. (2008). GMDD: A database of GMO detection methods. BMC Bioinformatics 9, 260. –9.WINDELS, P.,TAVERNIERS,I., DEPICKER,A., VAN BOCK- STAELE,E.and DE LOOSE,M.(2001):Characterizations of the roundup ready soybean insert. Eur.FoodRes. Tech. 213, 107–112. Author’s addresses Prof. Zoran T. Popovski PhD (corresponding author: zoran_popovski@yahoo.com), University “Ss. Cyril and Methodius” – Faculty of Agricultural Sciences and Food – Institute of Animal Biotechnology, Department of Biochemistry and Genetic Engineering, Bld. “Aleksandar Makedonski” –bb, 1000 Skopje, Republic of Macedonia; Ass. Prof. Elizabeta Miskoska-Milevska PhD, University “Ss. Cyril and Methodius” – Faculty of Agricultural Sciences and Food – Food Institute ,Department of Food Quality and Safety, Bld. “Aleksandar Makedonski” – bb, 1000 Skopje, Republic of Macedonia; Tome Nestorovski, University “Ss. Cyril and Methodius” –Faculty of Agricultural Sciences and Food –Institute of Animal Biotechnology –Department of Biochemistry and Genetic Engineering, Bld. “Aleksandar Makedonski” –bb, 1000 Skopje, Republic of Macedonia; Prof. Zlatko Pejkovski PhD, University “Ss. Cyril and Methodius” –Faculty of Agricultural Sciences and Food –Institute of Animal Biotechnology, Department of Technology of Animal Products, Bld. “Aleksandar Makedonski” –bb, 1000 Skopje, Republic of Macedonia.
Fleischwirtschaft international 1_2017 47 CALENDAR Calendar 24 –26February Yangon, Myanmar 26 February –2March Dubai, UAE 28 February –3March Moscow, Russia 2–5March Istanbul, Turkey 7–9March Rennes, France 7–10March Tokio, Japan 8March Copenhagen, Denmark 18 –20March Athens, Greece 21 –23March Ho Chi Minh, Vietnam 28. –30March Lagos, Nigeria Book review Profound insights for food industry and consumers HOOGENKAMP,H.(2017): Plant Protein &DisruptiveDiagnostics |The Transformational Food Journey for Today'sFuture | 450 p. |US$ 68,€64 | ISBN 9781534787421| www.amazon.com Myanmar Agriculture Expo, Myanmar Food Processing Expo MiTAServices Pte. Ltd. &MiTAMyanmar ( +95 9420 110 666) Gulfood Dubai World Trade Centre ( +971 4332 1000) Dairy &Meat Industry and Ingredients Russia ITE Moscow ( +7 499 750 08 28) Fotec ( +90 212 216 40 10) Cifa GL events Exhibitions ( +33 553367878) Foodex Japan Japan Management Association ( +81-3-3434-1238) Meat ShowHow Marel Meat ( +45 2082 7344) Food Expo Greece Metropolitan Expo ( +30 210 5242100) Pro Pack Vietnam 2017 SES Vietnam Exhibiton Services Co., Ltd ( +84 83930 7618) Nigeria agrofood fairtrade GmbH &Co. KG ( +49 6221 45 65 14) Insights about plant protein and disruptive diagnostics Henk Hoogenkamp's book “Plant Protein &Disruptive Diagnostics” tackles topics from food-related diseases to malnutrition to organic and GMO to dealing with a world approaching an epidemic of obesity. Anew nonfiction work shows the challenge of malnutrition in the developing world, even as Western societies are dealing with obesity. For most consumers in the Western world an abundance of animal protein is nearlyalways part of the dailydiet, while for most in the developing world not sufficient animal protein is available. Hoogenkamp argues that the key to solve this dilemma is unlocking the potential of plant proteins as well as cellular 3–6April Khartoum, Sudan 4–6April Chicago, USA 10 –13April Algier, Algeria 22 –24April Cairo, Egypt 25 –27April Brussels, Belgium 25 –27April Krasnodar, Russia 27 –28April Mumbai, India 27 –29April Istanbul, Turkey 4–10May Dusseldorf, Germany 8–11May 2017 Milano, Italy biotechnology that deliver affordable nutrition, improve health and wellbeing and reduce the environmental burden in an era of shrinking water and land resources. Along with detailed chapters discussing plant protein varieties such as derived from soy, pea, wheat, rice, potato and hemp the book explains: r Food, water &climate change r Sports Nutrition, Wellness & Lifestyles r Food: People, Plamnet, Profit r Glutenfree Protein Solutions r Societal Food r Diabetes T2: From Bad to Worse r Fast Good Food &Family r Fiber: ANatural Need for More r Lifestyle Diagnostics r Real Plant Meat r Sugar, Salt Phosphate: Less is More r Natural &Organic r Sarcopenia &Longevity AgriAfrica, FoodExAfrica Aldowalia Integrated Solutions & Management Projects (IMG) Co. Ltd. ( +249 994 824 977) ProFood Tech McCormick Exhibition Center ( +49 221 821-2960) Djazagro Algeria Safex Exhibition Park ( +33 176771489) Food Africa IFP Group ( +961 5959 111, -172) Seafood Expo Seafood Expo Global ( +1 207-842-5590) Food Industry Krasnador KrasnodarExpo ( +7 861 200-12-34) Fresh Produce India Asiafruit ( +44 20 7501 3702) VIV Turkey 2017 Hkf Fuarcilik A.S. ( +90 212 216 40 10) Interpack Messe Düsseldorf GmbH ( +49 211 456001) Tutto Food Fiera Milano SPA ( +39 02 4997 6239) Alle Veranstaltungen auch auf www.fleischwirtschaft.de Hoogenkamp is born in the Netherlands, in his entire professional career he has been ahead of the curve, many times more right than wrong. Many of the things he advocated for were initiallylooked upon skeptically, but are now standard procedure in the industry.With honesty and lots of inside information, Hoogenkamp gives afresh new voice to the world of plant protein technology and marketing. He shares practical know-how reflecting the skills needed to feed the world with food for tomorrow. Along with coining the term “Lifestyle Foods” in the 1990s, his resume includes pioneering work in developing groundbreaking applications for milk and plant protein ingredients in meatfree foods, cream liquers, and cheese analogs. //www.henkhoogenkamp.com
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