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LUI modificado<br />

- Se basa en la congelación (-20°C y –70°C ), el que produce un<br />

cambio estructural<br />

- Libera los anticuerpos y se usa principalmente para EHRN<br />

- Es de tiempo corto y facil<br />

- Solo recupera anticuerpos ABO<br />

TECNICA<br />

- GR lavados 6X<br />

- Colocar 4 – 6 gotas del GR al 50% en 3 o 4 tubos<br />

- Embeber las paredes del tubo<br />

- Congelar a -20 a -80°C (5 min)<br />

- Descongelar rápidamente<br />

- Centrifugar y transferir el eluido<br />

Edvin Santiago Trujillo, Email: edvinst2000@yahoo.es

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