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BLOQUEO<br />

Buffer de<br />

bloqueo<br />

Composición<br />

Desventajas<br />

Caseína<br />

Caseína/Tween20<br />

5% p/v leche sin grasa<br />

5% p/v leche sin grasa, O,2 %de<br />

Tween20<br />

Deteriora rápidamente.<br />

Enmascara algunos<br />

antígenos<br />

Deteriora rápidamente.<br />

Enmascara algunos<br />

antígenos<br />

Tween 20 O,2 %de Tween Podría generar algunos<br />

residuos<br />

Gelatina<br />

Gelatina 0.2% (2mg/ml)<br />

BSA 3% de BSA, Relativamente costoso

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