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Traitement des données : Excel pixel2 pixel2 ratios mitosox ratios nadhdate condition sum fluo mitosox area mitosox sum fluo <strong>NADH</strong> area <strong>NADH</strong> nbre noyaux sum/noyaux area/noyaux sum/noyaux area/noyaux21/06/2011 LG 24h 2523/06/2011 LG72h 98906114244653671226428/06/2011 HG50 24h 6198179 56432 2620178 31687 17 364598,765 3319,52941 154128,118 1863,94118HG50 I100 1121030 18741 4343448 32315 25 44841,2 749,64 173737,92 1292,6LG 24h 2470011 43667 7076394 62130 76 32500,1447 574,565789 93110,4474 817,5LG24hbis 2689154 39563 3135461 38962 45 59758,9778 879,177778 69676,9111 865,822222LG i100 24h 2856825 45583 2811644 35414 51 56016,1765 893,784314 55130,2745 694,392157LG i100 24hbis 1500217 25573 1646190 21369 36 41672,6944 710,361111 45727,5 593,58333330-juin HG50 72h 1344902 40027 3941416 56671 102 13185,3137 392,421569 38641,3333 555,598039HG50i100 72h 1794838 47574 4547415 60217 71 25279,4085 670,056338 64048,0986 848,126761LG 72h 3759318 80792 5031840 67795 124 30317,0806 651,548387 40579,3548 546,733871LG i100 72h 2003102 54767 5394824 72523 150 13354,0133 365,113333 35965,4933 483,48666705/07/2011 HG50 24h 10787714 95229 2500100 29757 22 490350,636 4328,59091 113640,909 1352,59091HG50i100 24h 1882751 40708 2818703 36449 22 85579,5909 1850,36364 128122,864 1656,77273LG 24h 564047 15073 2672600 34673 34 16589,6176 443,323529 78605,8824 1019,79412LG i100 24h 1864241 48622 3435295 46667 58 32142,0862 838,310345 59229,2241 804,60344807/07/2011 HG50 72h 276659 17802 95 2912,2 187,389474HG50i100 72h 275066 13789 3105454 52121 67 4105,46269 205,80597 46350,0597 777,925373LG 72h 1028149 42928 3526144 53997 128 8032,41406 335,375 27548 421,851563LG i100 72h 348299 22850 4464335 70034 127 2742,51181 179,92126 35152,2441 551,448819
Résultats : MITOSOX ratios sum fluo mitosox/nbre noyaux40003500300025002000150010005000LG LG i100 HG50 HG50 i10024h72hratios area mitosox/nbre noyaux120010008006004002000LG LG i100 HG50 HG50 i10024h72h
Page 1 and 2: Mesure de la production d’ion sup
Page 4 and 5: MICROSCOPIE ÉPI-FLUORESCENCE…
Page 6 and 7: M. Fallet cours de microscopie conf
Page 8 and 9: MICROSCOPE CONFOCAL LEICA TCS SP2 A
Page 10 and 11: Outils d’exploration du fonctionn
Page 12 and 13: Visualisation de l’ouverture du P
Page 14 and 15: Principes d’analyse d’images =
Page 16 and 17: L’apport de l’analyse images: Q
Page 20 and 21: Visualisation et Quantification de
Page 22 and 23: Quantification de la production de
Page 28 and 29: Analyse Volume et Intensité de Flu
Page 32 and 33: Résultats : NADH ratio sum nadh/nb
Page 34: CONCLUSION 2 :Les Limites de l’An

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